Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,755,871	0	T	A	40.0%	50.3 / 18.7	35	G58G (GGT→GGA)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base T (15/6);  new base A (0/14);  total (15/20)
Fisher's exact test for biased strand distribution p-value = 1.76e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAA‑CACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAAC  >  NC_000913/1755722‑1756003                                                                                                                                                       |                                                                                                                                     aCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAA‑CACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTa                                                                                                                                     <  2:58809/151‑1 (MQ=255)  ccATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAA‑CACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTaa                                                                                                                                    <  2:5040/151‑1 (MQ=255)   cATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAA‑CACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTaaa                                                                                                                                   <  1:225986/151‑1 (MQ=255)    aTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAA‑CACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTaaaa                                                                                                                                  <  2:292989/151‑1 (MQ=255)         aCCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAA‑CACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAAccgcc                                                                                                                             <  2:344916/151‑1 (MQ=255)           cGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAA‑CACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACcgccgc                                                                                                                           >  2:427937/1‑151 (MQ=255)                   gAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAA‑CACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGc                                                                                                                   >  2:307472/1‑151 (MQ=255)                          aaGAGATTTTAGTTAAAATGGGGGCCGCTGGAATGAACTTTGTGCGTCTAACCTTCTCTCAGGGTGACTATGAAAAA‑CGCGGTCAGCGCATTCAGAATCTGCGAAACTTGAGAAGAAAAACTGGAAAAACCGCCGCTATCCTGCTTg                                                                                                                <  1:208531/147‑1 (MQ=255)                          aaGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAA‑CACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATAc                                                                                                            >  2:156941/1‑151 (MQ=255)                                ttttagaaaaaaGGCGGGCCGCTGGCATGAACGTTATGCGTCTAACCTTCTCTCAGGGTGACAAGGCAAAA‑CGCGGCCAGCGCATTCAAAACCTGCGAAATTTAAGGACCAAACCTGGAAAACCCGCCGCTATCCTGCTTGATAcc                                                                                                           <  1:307413/138‑1 (MQ=255)                                  ttAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAA‑CACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTc                                                                                                    >  1:578538/1‑151 (MQ=255)                                                ggACGCTGGCATGAACTTTATGCGTCTAAACTTCTCTCAGGGTGACTAGGAAGAA‑CACGGTCGGCGCATTAAAAATCGGCCCAAGGGAAGGAAAAAAACGGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCg                                                                                          <  2:447276/147‑1 (MQ=255)                                                    gCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAA‑CACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATg                                                                                   >  2:208658/1‑150 (MQ=255)                                                      tGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAA‑CACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGaa                                                                                 >  1:8693/1‑150 (MQ=255)                                                        gCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAA‑CACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACt                                                                              >  1:321964/1‑151 (MQ=255)                                                            aaaCGTTATGCTTCTGATCTTCTTTCTGGGGGACTA‑GCAAAATCAAGTCCGGCGCATTCAGTAGCGGCAAAAAGGGAGAAAAAAAGCGGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCCCCATGAAACt                                                                              <  1:250263/146‑1 (MQ=255)                                                             aaCGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAA‑CACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGa                                                                           >  2:111730/1‑149 (MQ=255)                                                                 ttATGCGTCTGAACTTCCCTCATGGTGACTAGGAAAAA‑CCGGGCCACCCCATCAAAAACCTGCGCAACGTAAAGAGAAAAGCTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTgg                                                                            <  1:93476/144‑1 (MQ=255)                                                                      cttCTGACCTTCTCTCATGGTGACTATGCAAAA‑CACGGTCAGCGCATTCAGATTCTGCGCACCGTGAGGAAAAAAACTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGt                                                                    <  2:375746/145‑1 (MQ=255)                                                                       gtttaatctcctctcGGGGTGACTAGGAAAAA‑CCCGGCCAGCGCATCCAGAATCGGCGCAAGGGGATGAAAAAAAGGGATAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAacga                                                               <  1:525942/141‑1 (MQ=255)                                                                               ttCTCTCATGGTGACTATGCAGAA‑CACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTtctc                                                       >  2:425911/1‑151 (MQ=255)                                                                                     cagggtgataacccAGAA‑CGCGGGCACCGCATCCAAAATCGGCCCAACGTGAGAACAAAAACGGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGttt                                                          <  1:34690/129‑1 (MQ=255)                                                                                     atgggtatcaaggCAAAA‑CACGGTCGCCACATTAAAACTCGGCGAATCGGGATAAAAAAACGTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGaa                                                   <  2:198707/137‑1 (MQ=255)                                                                                        ggTGACTATGCAGAA‑CACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTg                                              >  2:282951/1‑151 (MQ=255)                                                                                                   aaaa‑CGCGGCCAGCCCATCAAAAACCTGCAAAACGTGATAAAAAAAACGGAAAAACCCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCtt                                    <  1:365970/148‑1 (MQ=255)                                                                                                    gAA‑CACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCttt                                   >  1:156642/1‑150 (MQ=255)                                                                                                    aaaccccGGTCAGCGCATTAAGAATCGGCGCACCGTGAGGAGAAAACCTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGAc                                       <  2:571569/141‑1 (MQ=255)                                                                                                      accacGGTCGGCTCATTCAAAACCTGCGCAGCGGAATGAGAAAAATTGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCt                                     <  2:228729/145‑1 (MQ=255)                                                                                                           ggccAGCGCATTCAGAATCTGCGCAACGTGAGGAAAAAAACTGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCttt                                   <  1:380328/141‑1 (MQ=255)                                                                                                                      taaaattcggcaaaaCGTGAGGAAAAAAACTGAAAAACCCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGa                     <  1:226841/134‑1 (MQ=255)                                                                                                                       cAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAAt                >  1:245269/1‑151 (MQ=255)                                                                                                                          aaTCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGACACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTg             >  1:453809/1‑151 (MQ=255)                                                                                                                                    aCGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCa    >  1:426073/1‑150 (MQ=255)                                                                                                                                      gTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAAc  >  2:324208/1‑150 (MQ=255)                                                                                                                                            agaaaaaCTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCaa   <  2:467062/140‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                     ACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAA‑CACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAAC  >  NC_000913/1755722‑1756003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑