Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,764,7850TA41.9% 34.7 / 13.9 31intergenic (‑399/+149)sufA/ydiHFe‑S cluster assembly protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (9/9);  new base A (0/13);  total (9/22)
Fisher's exact test for biased strand distribution p-value = 3.60e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAACTTGCGGCAT  >  NC_000913/1764637‑1764923
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tAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATa                                                                                                                                           <  2:562466/150‑1 (MQ=255)
 aaGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATaa                                                                                                                                          <  2:466032/150‑1 (MQ=255)
  agagATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAg                                                                                                                                        <  2:535893/151‑1 (MQ=255)
    agaTAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCt                                                                                                                                      <  2:66859/151‑1 (MQ=255)
            aaaaaaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACccgcc                                                                                                                              <  2:51153/151‑1 (MQ=255)
                     cAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAac                                                                                                                      >  1:350875/1‑150 (MQ=255)
                       gCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAacac                                                                                                                    >  1:406437/1‑150 (MQ=255)
                               aaaaaaaaaaCCCCTTAAGTCTGTGCGACACAGGTTTAAGGTTTTCTCCCCCACCCGGCGCTTATCCCCGGCCCTCTCAGTGGCTTAGCTCTTGAAGGGGCGAAAAAAATAATCTAATAAAGCTAACCCGCCGTTTTAACACAAACTGc                                                                                                             <  2:511367/149‑1 (MQ=255)
                                 aTAAAAAACCCCTTAAGTCTGTGCGACACAGGCTTAAGGTTTTCTCCCCCACCCGGCGCTTATCCCCGGCACTCTCAGTGGTTTAGCTCTTGAAGGGGCGATAAGAATAATCTCAAAAAGCTAACCCGCCGTTTTAACACAAACTGCGAtt                                                                                                         <  2:231373/151‑1 (MQ=255)
                                          cctttAAGTCGTTGCAACACAGTTTTAAGGTTTTTACCCCCACCCGGCGCTTATCCCCGGCCCTCTCAGTGGCTTAGCTTTGGAGGGGGCGAAAAAAATAATCTCAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGtattat                                                                                                 <  2:375842/147‑1 (MQ=255)
                                          cccTTAAGTTGGTGCAACAAGGGTTTAAGGTTTTCTCCCCCACCCGGCTTTTATCCCCGCCCCTCAAAGTGGTTTAGCTTTTGAAGGGGAGAAAAAAATAATCTCAAAAAGCAACCCCGCCGTTTTAACACAAACTGCGATTAGtatta                                                                                                  <  1:477494/149‑1 (MQ=255)
                                                   cggtgCGACACAGGCTTAAGGGTTTCTCCCCCACCCGGCGCTTATCTCCGGCACTCTCAGTGGTTTAGCTCTGGAAGGGGCGATAAGAATAACCTAAAAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGaa                                                                                          <  2:318299/146‑1 (MQ=255)
                                                    tgggcaacacGGGTTTAGGGTTTTCTCCCCCATCGGGCGTTTATCCCCGGCCCTCTAGGTGGTTTAGCTTTTGAAGGGGAAAAAAAAATAATTTAAAAAGGCAAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAAc                                                                                         <  1:32104/142‑1 (MQ=255)
                                                     ggccGACACAGGTTTAGGGGTTCCCCCCCCACCCGGCGCTTATCCCCGGCCCTCTCAGTGTTTTACCTTTTAAGGGGGAAAAAAAAATAATTAAAAAAATTTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAat                                                                                      <  1:448246/147‑1 (MQ=255)
                                                      tgCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAatat                                                                                    >  2:434285/1‑151 (MQ=255)
                                                          acacAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAgg                                                                                >  1:414236/1‑151 (MQ=255)
                                                           cacaGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGc                                                                               >  1:125622/1‑151 (MQ=255)
                                                            acaGGTTAAAGGGTTTCTCCCCCACCCGGCGCTTATCTCCGCCACTCTCAGTGGTTTAGCTCTTGAAGGGGCAATAAAAATAATCTAATAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGc                                                                               <  2:178430/150‑1 (MQ=255)
                                                                       gggttCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGc                                                                    >  1:235252/4‑150 (MQ=255)
                                                                                        gcgcTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATc                                                  >  2:542628/1‑151 (MQ=255)
                                                                                        gcccTTATCTCCGACCCTCTCAGTGGCTTAGCTTTTGAAGGGGAAAAAAAAAAAATTTAAAAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCa                                                    <  1:161162/146‑1 (MQ=255)
                                                                                            ttACCTCGGGCACTCTCAGGGGTTTAGCTCTTGAGGGGGGAATAAGAATAATCTAATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGGACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGa                                                <  2:168145/149‑1 (MQ=255)
                                                                                                ccccgcccctctCAGTGGTGTAGCTCTGGAAGGGGAAAAAAGAATAATCACAAAAATCAACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGa                                                <  1:547503/137‑1 (MQ=255)
                                                                                                ccccGGCACTCTCAGTGGCTTACCTCTGGAGGGGGCAAAAAAAAAAATCTCAAAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTCTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTg                                           <  2:235243/148‑1 (MQ=255)
                                                                                                           tcAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTa                                >  1:65521/1‑150 (MQ=255)
                                                                                                                    tagctatgaaaGGGGCAAAAAAAAAAATCTAAAAAAGCAAACCTGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAg                          <  1:451542/138‑1 (MQ=255)
                                                                                                                               ggggCAAAAAAAATAATCCAAAAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAAc           <  2:449474/151‑1 (MQ=255)
                                                                                                                                 ggaaaaaaaaataatCTAATAAAGCAAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAACtt         <  1:288687/142‑1 (MQ=255)
                                                                                                                                     aaaaGAAAAATCTAAAAAAGCAAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAACTTGCgg     <  1:397848/149‑1 (MQ=255)
                                                                                                                                       aaaaaaaatCTTAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAACTTg        <  1:137777/140‑1 (MQ=255)
                                                                                                                                        aGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAACTTGCGGCAt  >  2:222019/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                          
TAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAACTTGCGGCAT  >  NC_000913/1764637‑1764923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑