| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,575,947 | 0 | A | C | 52.2% | 31.7 / 39.0 | 25 | V372G (GTT→GGT) | gntU | gluconate transporter, low affinity GNT 1 system |
| Reads supporting (aligned to +/- strand): ref base A (9/2); new base C (0/12); total (9/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.73e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.04e-05 | |||||||||||
GGCTTCGGTCGCGCCGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCC > NC_000913/3575811‑3576092 | ggCTTCGGTCGCGCCGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGt > 1:383215/1‑151 (MQ=255) cGGTCGCGCCGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTAc < 2:497350/151‑1 (MQ=255) ggTCGCGCCGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTAcc < 2:580473/151‑1 (MQ=255) aacccGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCCCCGGAGTATTTCAGTTGTTCAATACCCGGCATCCCCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAAccc < 2:8710/147‑1 (MQ=255) tGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGAGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGc > 1:360444/1‑149 (MQ=255) tGACAACAACCAACCCACCAGCGATACAAATCGACGGCGCCCCCATTTGCGCACCGGAGTAGTTCATTTGTTCAATAGCCGGCACCCCCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCt < 1:147083/149‑1 (MQ=255) ccaacccaccgGAGAAAAAAACCGCCCGCGCCCCCTTTTGCGCACGGGAGTAGTTCAGTTTTTCAATACCCGCCACCCCCAGTCCGACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGa < 1:529395/145‑1 (MQ=255) cccccaGCGATACAAAGCGCCCGCGCCGCCATTTGCGCCCCGGAGTAGTCCAGTTTTTCAATACCCGGCATCCCCGCTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAgg < 1:271452/148‑1 (MQ=255) ccccaGCAATACAAATCGACGGCCCCGCCATTTGCGCCCCGGGGTAGTTCAGTTTTTCAATACCCGGCATCCCCAGCCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGt < 1:281307/149‑1 (MQ=255) aGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGAt > 1:7863/1‑150 (MQ=255) aCAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGcc > 2:518090/1‑151 (MQ=255) aGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGccc > 2:138322/1‑150 (MQ=255) ccccttttGCGCCCGGGAGTAGTACAGTTGTCAAATACCCGCCACCCCCGGCCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGcc < 1:95937/139‑1 (MQ=255) gccttgcccgcCCCGGAGTAGTTCTGTTGTAAAATACCCGGAATCCCCAGTCCAACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCg < 1:291381/142‑1 (MQ=255) aTTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGtt > 2:473113/1‑150 (MQ=255) ttggcccACGGGAGTAGTTCAGTTGTTCAATACCCGGCATCACCATTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGtt < 2:274508/143‑1 (MQ=255) ttGCGCGCCGGAGTAGTTCATTTGTTCAAAACCCGGCATCCCCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCGAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTaa < 1:309752/150‑1 (MQ=255) gcgcACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGc > 2:520700/1‑151 (MQ=255) ccccccGGAGTATTTCAGTTGTTCAATAGCCGCCATCCCCATTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCt < 2:181795/147‑1 (MQ=255) ccccGGAGTATTTCATTTTTTAAATACCCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTc < 2:208082/149‑1 (MQ=255) ggAGTAGTCCAGTTGTCCAATACCCGGCACCCCCAGCCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGc < 1:511840/144‑1 (MQ=255) tgttCAATACCCGGCATCCCCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCt < 2:347982/150‑1 (MQ=255) ttaaaTACCCGGCATCCCCACTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTa < 2:66484/146‑1 (MQ=255) tCAATAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGc > 1:308314/1‑151 (MQ=255) aTAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGcc > 2:453142/1‑149 (MQ=255) | GGCTTCGGTCGCGCCGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCC > NC_000913/3575811‑3576092 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |