Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,745,4940CT38.9% 45.2 / 10.5 37R231C (CGC→TGC) yiaN2,3‑diketo‑L‑gulonate TRAP transporter large permease protein
Reads supporting (aligned to +/- strand):  ref base C (6/16);  new base T (14/0);  total (21/16)
Fisher's exact test for biased strand distribution p-value = 1.32e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGTT  >  NC_000913/3745346‑3745642
                                                                                                                                                     |                                                                                                                                                    
gggCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTc                                                                                                                                                      <  2:47592/149‑1 (MQ=255)
       ccGCTGATTCTTCCCTGGTGGTGGCTGGCCACTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCGCTCCGGcc                                                                                                                                             <  2:439169/150‑1 (MQ=255)
               gCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTGCTCCCGCCCGTTTTcc                                                                                                                                     >  2:24815/1‑151 (MQ=255)
                    ccTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTGCTCCCGCCCGTTTACCCCTAc                                                                                                                                >  2:389628/1‑151 (MQ=255)
                      tggtggtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTGCTCCGGCCTGGTTTcccc                                                                                                                                   >  2:538374/1‑146 (MQ=255)
                             ggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCgg                                                                                                                       <  2:24215/151‑1 (MQ=255)
                                 ggccgtacGCCTTATTTGCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGcc                                                                                                                   <  1:501347/144‑1 (MQ=255)
                                 gcccAGTCGCTTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGcc                                                                                                                   <  1:566898/149‑1 (MQ=255)
                                         gCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGACATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTGTCGCTTCGGCCTGGTTTTTCCTACCGCAGACGGGGCCGGtgct                                                                                                             >  1:115185/1‑149 (MQ=255)
                                              aaTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCt                                                                                                      <  2:169908/151‑1 (MQ=255)
                                              aaTTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCGCCCCGGCCTGTTTATCCCCACCGAAGGCGGGGCCGTTGCTGCTTTCt                                                                                                      >  2:207237/1‑151 (MQ=255)
                                               aTTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTTGCTCCGGCCTTTTTTTCCCCACCCAATACCCGGCGGCTGCCGCTTTCt                                                                                                      >  1:237541/1‑150 (MQ=255)
                                                tttACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGGCT‑CGGGCTGGTTGTCCCTTACGAAGCCCGGGGCGGtggttgtt                                                                                                         >  1:454934/1‑140 (MQ=255)
                                                    ccccGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTTGCTCCGGCCTGTTTTCCCCTACCCAAGCCGGGGCCGTTGCTGCTTTTTATtcg                                                                                                 >  1:196152/1‑150 (MQ=255)
                                                       cGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTCTCTTCCAGTCATCATTATTGGC‑GGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGt                                                                                             <  2:542664/151‑1 (MQ=255)
                                                        gCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGtt                                                                                            <  1:183858/151‑1 (MQ=255)
                                                         ccAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTTGCTCCGGCCTGTTTTCCCCCACCCAAACCGGGGCCGTTGCTGCTTTTTATGCGCTGttt                                                                                           >  1:443544/1‑151 (MQ=255)
                                                             cAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCGCTCCGGCCTGTTTTCCCCCACCGAAGCCGGGGGCGTTGCTGCTTTTTATGCGCTGttt                                                                                           >  2:465511/1‑147 (MQ=255)
                                                                    cAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTGCTCCGGCCTGTTTTTCCCCACCGAAGCCGGGGCCGTTGCTGCTTTTTTTGCGCTGGTTTTCGcccc                                                                                   >  1:477995/1‑146 (MQ=255)
                                                                        gATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTAtt                                                                            <  2:116745/151‑1 (MQ=255)
                                                                                aaaTCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTGCTCCCGCCTGTTTTCCCCCACCGAAACCGGGGGCGTTGGTGCTTTCTTTGCGCTGTTTTTTGCCCCCGTTTTTTAcc                                                                        >  1:208961/1‑147 (MQ=255)
                                                                                  aTCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTGCTCCGGCCCGTTTTCCCCTCCCCAAGCCCGGGCCGGTGCTGCCTTCTTTTCGCTGTTTGTCGCCCCAGTTTTTTTCCGCGaa                                                                   >  1:458293/1‑150 (MQ=255)
                                                                                   tCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTTTTCCGGCCTGTTTAACCCTACCTAAGGCAGGGACGGTGGTGCTTTTTATGCGCTGGTTTTCGGCACAGGTTTTTATCGttgaa                                                                  >  2:35860/1‑146 (MQ=255)
                                                                                      ggCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGaaa                                                                  <  2:26106/147‑1 (MQ=255)
                                                                                        cACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCGCTCCGGCCTGTTTTCCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTTTCGCCACAGTTATTTTCCGTGAAATGAcc                                                             >  2:275865/1‑150 (MQ=255)
                                                                                              tttGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGc                                                        <  2:111448/149‑1 (MQ=255)
                                                                                                   ctcCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCTCTCCGGCCTGTTTATCCCCACCCAAACCGGGGGCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCCCCCTTTTTTACCGTGAAATGACCTTCGCCccac                                                   >  1:75238/1‑149 (MQ=255)
                                                                                                        gTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTTGCTCCGGCCTGTTTTTCCCCACCGAAACCGGGGGCGGTGGTGCTTTTTATGCGCTGTTTTTTGCCACACTTATTTTCCGTGGAAAGACCTTTGCCCCACttttggt                                            >  1:4687/1‑147 (MQ=255)
                                                                                                          aTCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTGCTCCGGCCCGTTTTTCCCCACCCAAACCGGGGCCGGTGCTGCCTTTTATTCGCTGTTTTTTGCCACAGTTATTTACCCTGGAAAGACCTTCGCCACACTTTGGcct                                           >  2:194471/1‑148 (MQ=255)
                                                                                                           tCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTGCTCCGGCCTGTTTTCCCCCCCCGAAACCCGGGGCGGTGCTGCTTTCTTTGCGCTGTTTTTCGCCACAGTTATTTTTCGCGAAAAGACCTTCGCCACACTTTTGGATGt                                         >  1:130177/1‑151 (MQ=255)
                                                                                                              gggCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTAc                                       <  1:465418/150‑1 (MQ=255)
                                                                                                                gcgcTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCa                                    <  1:210374/151‑1 (MQ=255)
                                                                                                                     gTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTg                                 <  1:70411/149‑1 (MQ=255)
                                                                                                                        tcttcCAGTCATCATTATTGGC‑GGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGgcgc                             <  1:117122/150‑1 (MQ=255)
                                                                                                                           tcCAGTCATCATTATTGGC‑GGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGcgcg                            <  1:539792/148‑1 (MQ=255)
                                                                                                                              aGTCATCATTATTGGC‑GGGTTTTGCTCCGGCCCGTTTTTCCCTACCCCAGCCGGGGGCGGTTCTGCTTTCTATGCGCTGTTTTTTGCCACAGTTATTTTCCGCGAAATGACCCTCGCCCCCCTTTTGGCTGTACTCATTTGCgcgggg                         >  1:338847/1‑146 (MQ=255)
                                                                                                                                           ggC‑GGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTg          <  1:240521/150‑1 (MQ=255)
                                                                                                                                                   ttCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGtt  >  2:111999/1‑151 (MQ=255)
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GGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGTT  >  NC_000913/3745346‑3745642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑