Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913606,6190TC52.0% 12.1 / 28.2 25G255G (GGA→GGGybdKweak gamma‑glutamyl:cysteine ligase
Reads supporting (aligned to +/- strand):  ref base T (8/4);  new base C (0/13);  total (8/17)
Fisher's exact test for biased strand distribution p-value = 4.58e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.90e-03

CTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGCTGTCGATCATCGTGGTGTAACTCAGACAGCGAAA  >  NC_000913/606504‑606762
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cTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACgg                                                                                                              >  1:122250/1‑151 (MQ=255)
    aacccaatggcgcCAGTCGTGAAAAGGTTTAAATTTATACAGAAGTTATTTTTTTCCTTGATTTTTAACCGGGGGTTCCGTCAGTACCGGGTGGGGGGTGGCCTAAATAATCCCCGCCATATTTACTGCGTGGCTAAGGGTTAACgg                                                                                                              <  1:392948/138‑1 (MQ=255)
        ccATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCAAATTTACTGCGTGGCTAAGGGTTAACGGGGTATcc                                                                                                       >  2:56280/1‑150 (MQ=255)
                 cAGGCGTGAAAACGTTAGAATTAACACGGCGGTTATTTTTTTTCCTGTTGTTAAAGCGGCCCTCCGTTCGGTAAAGGGGGGGGGGTAGCATAATTTACCCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCg                                                                                               <  1:279001/149‑1 (MQ=255)
                   gccgtaaaaaCGGTTGAATTTAACCAGCAGTTATTTTTTTTCTTGTTGTTTAACCGGCCGTCCCTTCAGTAACCAGGGGGGGGTAGCCTGAATTACCCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAAc                                                                                            <  1:102382/143‑1 (MQ=255)
                        gaaatcGGTTGATTTTATACAGAAGGTATTTTTTTTCTTGTTTTTGAAGGGGGCGTCCCGTCATTAACCGGTGGGGGGTAGCGTGATTCAACCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCa                                                                                       <  1:65652/145‑1 (MQ=255)
                        gaaaaGGGTTAATTTTATACAGCGGGTAATTTTTTCCCTGTTGTTAAACGGGGCGTCCCGTCAGAACCCAGGGGGGGGTGGCTTAAATAAACCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCa                                                                                       <  2:195699/148‑1 (MQ=255)
                          aaagggtaaaaTTTAAACAGAAGTTATTTTTTTCCTTATTTTTTAACGGGGCGTCCCGTCAGAACCCGGGGGGGGGTAGCTTAAATTAACCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCAcc                                                                                     <  2:214124/141‑1 (MQ=255)
                              ggTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGcc                                                                                >  2:19135/1‑151 (MQ=255)
                                        ataCAGCAGGTATTTTTTTCCCTGATGTTTAACGGGGCGTTCCGTCAGTAACCGGGGGGCGGTGGCCTAAATAAACCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAg                                                                        <  2:6886/149‑1 (MQ=255)
                                          aCAGCAGGTATTTTTTTTCCTGTTTTTAAACGGGGCGTTCCTTCAGTAACCAGGGGGGGGTAGCCGGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAgg                                                                       <  1:189303/148‑1 (MQ=255)
                                                 gTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCg                                                              >  1:208950/1‑150 (MQ=255)
                                                      ttttttCCCTATTGTTAAACGGGGCTTTCGTTAAGTAACCAGGGGGGGGTACCTAAAATTAACCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAata                                                          <  2:172518/149‑1 (MQ=255)
                                                      cTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATc                                                        >  2:278881/1‑151 (MQ=255)
                                                       tttttCTTGATTTTTACCCGGCCGTTCCGACAGTACCGGGTGGGGGGGAGCCTAAATTACCCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATc                                                        <  1:322935/150‑1 (MQ=255)
                                                       tttttCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATcc                                                       >  1:40740/1‑151 (MQ=255)
                                                              tGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGc                                                 >  2:147958/1‑150 (MQ=255)
                                                                           gggCTTCCCATCAGTACCCAGGGGGCGGTGCCGTAAATAAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATg                                     <  2:1742/150‑1 (MQ=255)
                                                                            ggCGTCCCGTAAGTAACCGGGGGGGGGTAGCCTAAATTAACCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGCt                                   <  2:209036/151‑1 (MQ=255)
                                                                                          atcggggggtgggTAGCTTAATTTACCCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGCTGTCGatcatc                         <  1:35314/136‑1 (MQ=255)
                                                                                           acgaGTGGGGGGTTCCCGGAATTCACCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGCTGTCGATCATCgtg                      <  1:362956/146‑1 (MQ=255)
                                                                                                 gggCGGTAGCCTAAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGCTGTCGATCATCGTGGTGTAACt              <  2:210786/151‑1 (MQ=255)
                                                                                                       tAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGCTGTCGATCATCGTGGTGTAACTCAGAc         >  2:137767/1‑150 (MQ=255)
                                                                                                          cttaatttATTCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGCTGTCGATCATCGTGGTGTAACTCAGACAg       <  2:179720/143‑1 (MQ=255)
                                                                                                              aaTTAACCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGCTGTCGATCATCGTGGTGTAACTCAGACAGCGaaa  <  2:396227/150‑1 (MQ=255)
                                                                                                                    |                                                                                                                                               
CTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAACCTCCACCGTGCCAAAATGAGGACTGGGGCGAATATCCCAGTGCAGATCTTTAATGCTGTCGATCATCGTGGTGTAACTCAGACAGCGAAA  >  NC_000913/606504‑606762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: