| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,578,143 | 0 | A | C | 53.8% | 3.9 / 12.0 | 13 | L79V (TTA→GTA) | maeB | malic enzyme: putative oxidoreductase/phosphotransacetylase |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-03 | |||||||||||
CGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCG > NC_000913/2577999‑2578281 | cGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCa < 1:10795/150‑1 (MQ=255) cgACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATGACCGGTTTGCGTGCCAGCGCGCCAATGGTGCCCAACCCCAGCAccgcc > 1:33081/1‑149 (MQ=255) aaTAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTag > 2:299971/1‑149 (MQ=255) aaTAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTAAAATACATCAACCCCGGCAATTTTCTTAAACAGACCGCCTTTGCCTTCCATCCCGGGTTTGCCTGCCAGCGCGCCAATGTTGCCTACCCCCAGCACCGCCGTACCGTTag < 2:331460/149‑1 (MQ=255) aaaTTTGTCGGGGTCGATTTCGTCAACTTCAAGGTAAAAGACATCAACCCCGCCGAATTTCATAAACAAAACGCCCTTGCCTCCCATCCCGGGTTTGCCTGCAACCGCGCCAATGTTGCCAACCCCCAGCACCGCCGTACCGTTAGAGATc < 1:239593/151‑1 (MQ=255) cGTCAACTTCAATGTAAAATACACCAACCCCGGCGAATTTTTAAAACAAACCCCCCTTCCCTTCCACCCCCGTTTGGCCTGCCAGCGCGCAAATGTTCCCAACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTc < 2:275155/150‑1 (MQ=255) tCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGt > 1:330677/1‑151 (MQ=255) aaaCAGACCCCCCTTCCCTTCCACCCCCGGTTTGCCCGCCAGCGCGCAAATGTTGCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTc < 2:165994/150‑1 (MQ=255) aaaCAGACCCCCCTTCCCCCCCTCCCCCTGTTTCCCTGCCGGCCCCCCAATGTGCCACACCCCCGGCTCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCa < 1:246435/151‑1 (MQ=255) ccccGTTTTCCCTGCCAGCCCGCAATTGTTCCCACCCCCCACCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAAc < 2:145346/142‑1 (MQ=255) ttcccgcccAGCGCGCAATTGTTCCCAACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAg < 2:330809/143‑1 (MQ=255) ccAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAgcgc > 1:131263/1‑151 (MQ=255) aaTGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATGACCGCAACCAGGTTACATCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATcgcg > 1:98028/1‑150 (MQ=255) | CGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCG > NC_000913/2577999‑2578281 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |