Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,285,8530GT43.3% 26.4 / 14.1 30A126S (GCC→TCC) agaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
Reads supporting (aligned to +/- strand):  ref base G (2/15);  new base T (13/0);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 1.75e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

CGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCA‑GGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGCTGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGC  >  NC_000913/3285716‑3285998
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cGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCA‑GGGaa                                                                                                                                       <  1:157165/150‑1 (MQ=255)
        gaaAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAAATTTCTCATCATTTTCCAGCCA‑GGGAAACCCGCTgg                                                                                                                              >  2:314346/1‑151 (MQ=255)
           aaaGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAGATTTTTCCTTCTTTTCCAGCCC‑GGGGAAACCGCTtggg                                                                                                                            >  1:39093/1‑150 (MQ=255)
                agaAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGGATTTTGTCATCATTTTCCAGCCA‑GGGGAACCCGCTGGGGGCGGtt                                                                                                                      >  2:278389/1‑151 (MQ=255)
                    aaCCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGGAGTTGCTACTCTTTTTCCTACCC‑GGGGAAACTTCTGGGGGCggggctct                                                                                                                  >  1:106241/1‑144 (MQ=255)
                       cgcgACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTTCTCCTCCTTTTTCCGCCC‑GGGGAAACTGCTGGGGGCGGTTCTAtttt                                                                                                               >  2:152468/1‑151 (MQ=255)
                       cgcgACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCA‑GGGAAACCTGCTGGGGCCGATTCTAtttt                                                                                                               <  2:97249/151‑1 (MQ=255)
                        gcgACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGGATTTTCTCATCATTTTTCAGCCC‑GGGGAAACTGCTGGGGGCGGTTCTAttttt                                                                                                              >  2:62551/1‑151 (MQ=255)
                         cgACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGGAATTTTTCCTCCTTTTTCCGCCC‑GGGGAACCCGCTGGGGGCGGtttttttttt                                                                                                              >  2:144706/1‑150 (MQ=255)
                          gACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAAATTGTTCGTCATTTGTCCGACC‑GGGGAACCCGCCAGGGGCCATTGTAttttt                                                                                                              >  2:274172/1‑149 (MQ=255)
                            cacCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGGATTTTGTCCTCCTTTTTCAGCCA‑GGGGAACCTGCTGGGGGCGGGTCTtttttt                                                                                                              >  1:23898/1‑147 (MQ=255)
                            cacCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGGATTTTCTTCTCATTTTTCAGACA‑GGGGAAAATGCTGGGGGCGGGTTTAtttttttc                                                                                                           >  2:196912/1‑147 (MQ=255)
                                 ttAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGGATTTTCTCATCATTTTCCAGCCC‑GGGGAACCCGCTGGGGGGGGTTCTTTTTTTTTCCGtttt                                                                                                     >  2:248125/1‑150 (MQ=255)
                                           cAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCA‑GGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATc                                                                                           <  2:209009/151‑1 (MQ=255)
                                                  gCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCA‑GGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTcc                                                                                    <  2:345593/151‑1 (MQ=255)
                                                          tGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCA‑GGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGt                                                                             <  1:388997/150‑1 (MQ=255)
                                                                       gggATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGGGGTTTGTCATTATTTGTCACGCA‑GGGAGAACAGCTGGGGGGGGTTCTTTTTTTTTTCGTTTTTCTTCTTTTCTTTTTTCTTCGCGTCGGCGGGGcccccc                                                               >  1:107069/1‑148 (MQ=255)
                                                                          aTTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGGAATTTCTCTTCATTTTTCAGCCAGGGGAAACCT‑CTTGGGGCGGTTTTATTTTTTGCCCTTTACCCGCTTTTCTTTTTTCTGCGcggcgggcggagcc                                                                  >  2:72674/1‑137 (MQ=255)
                                                                                 atgcgatTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCA‑GGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGCTGGACCCACGTCGGTTATTc                                                     <  2:26890/151‑1 (MQ=255)
                                                                                        tttCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCA‑GGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGCTGGACCCACGTCGGTTATTCAGTCgg                                               <  2:35364/150‑1 (MQ=255)
                                                                                              gTTTACTTTGTTGCCGATTATGGCGGGAATTTTCTCATCATTTTCCAGCCC‑GGGGAAACTGCTGGGGCCGGTTCTTTTTTTTGCCGTTTTCCTGCTTTTTTTTTTTCTTCGCGGCGGCTGGACCCACGTCGGGTATTTAGTCGGCggg                                           >  2:365978/1‑146 (MQ=255)
                                                                                                 tACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCA‑GGGAAACCTGCTGGGGCAGATTCTATTTTTCGCCGTTTACCTACTTATCTTATTCCTGCGCGTCGGCTGGACCCACGTCGATTATTCAGTCGGCGTGAAGGc                                      <  1:375951/150‑1 (MQ=255)
                                                                                                  aCTTTGTTGCCGATTATGGCGGGGAATTTCTCCTCATTTTCCCGCCA‑GGGGAAACTGCTTGGGGCGGTTCTTTTTTTTTCCGGTTTCCTTCCTTTCTTTTTTCTTCGCGTCGGGCGGGCCCACCGCGGGTTTTTTGTCGGCGGGGAgggg                                     >  2:76289/1‑148 (MQ=255)
                                                                                                     ttgttgCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCA‑GGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGCTGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGcgatc                                  <  2:381015/150‑1 (MQ=255)
                                                                                                       gttgCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCA‑GGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGCTGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGcgatcgat                               <  2:140516/151‑1 (MQ=255)
                                                                                                                tATGGCGGGAATTTGCTCATCATTTGCCAGCCA‑GGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGCTGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCg                       <  1:152397/150‑1 (MQ=255)
                                                                                                                 aTGGCGGGAATTTGCTCATCATTTGCCAGCCA‑GGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGCTGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCga                      <  1:294090/150‑1 (MQ=255)
                                                                                                                             tGCTCATCATTTGCCAGCCA‑GGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGCTGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAg          <  2:86591/150‑1 (MQ=255)
                                                                                                                                tcaccaTTTGGCCCCCC‑GGGAAACCTGCTGGGGCCGATTCTATTTTGCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGCTGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAga         <  1:123187/148‑1 (MQ=255)
                                                                                                                                    catTTGCCAGCCA‑GGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGCTGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGc  <  2:7810/151‑1 (MQ=255)
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CGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCA‑GGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGCTGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGC  >  NC_000913/3285716‑3285998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑