Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,336,901 | 0 | T | A | 52.6% | 7.6 / 25.0 | 19 | Y44F (TAT→TTT) | adiC | arginine:agmatine antiporter |
Reads supporting (aligned to +/- strand): ref base T (5/4); new base A (1/9); total (6/13) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.73e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.75e-03 |
CAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATC‑GAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCAT‑AAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGC > NC_000913/4336751‑4337051 | cagTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATC‑GAGAGCCCCAGTGCACCGATAATCGTCACCAAccatccat > 2:214729/1‑151 (MQ=255) agTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATC‑GAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCAT‑a < 1:357750/151‑1 (MQ=255) gTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATC‑GAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCAT‑a < 1:11946/150‑1 (MQ=255) tGAAAACCGAGAAACGGGCAAAAGCAGCGGCGGGCGTAAGCGTAAGAACCGCCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATC‑GAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCAT‑AAATGGCAATCCCGCCa < 1:152028/150‑1 (MQ=255) aggcaggGGGGGGGGTAAGCGTAAGAACCACCACGACTTGGGTCGGGGAACGACATTTGGGCGTATACCATC‑GAGGGCCCCAGTGCACCGATAATCGTCTCCACCCATCCAA‑AAAGGGCAGTCCCGCCAGTAGAGGCCAGGTTTGCAGGTa < 1:303611/149‑1 (MQ=255) ggcgggcgTAAGCGTAAGAACCCCCGGAACTTGGGTCGAGGAACGCCATTGTGGCGTATACCATC‑GAGAACCCCAGTGCAGCGAAAATCCGCACCACCCATCAAA‑AAAGGGCAGCCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGaaa < 1:25187/151‑1 (MQ=255) gcgaaagcgtaaGACCCCCCAGGACTTGGGTCGAGGACCGACTTTTTGGCGTATCCCATC‑GAGACCCCCAGTGCCCCGAAAATCGCCCCCAACCATCCAA‑AAAGGCCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAacac < 2:176731/151‑1 (MQ=255) gcttaaaAACCCCCAGGTTTGGGGCCGGGAAACGCCATTTTGGCGTAACCCAAC‑GAGAGCCCCACGGCCCGGACAATGGTCACCAACCACCCAA‑AAATGACAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGa < 2:137347/146‑1 (MQ=255) accaccAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATC‑GAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCAT‑AAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCataat > 2:154634/1‑150 (MQ=255) tggggACGAGAACCGACATTTTGGGGTATCCCATG‑GAGAGCCCCAGTGCACCGTTAATCGCCCCCACCCATCAAA‑AAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGaca < 2:202324/148‑1 (MQ=255) gAGGACCGACATTTGGGCGTATCCCATC‑GAGACCCCCAGTGCCCCAATAATCGTCCCCACCCATCCAA‑AAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCa < 2:82781/149‑1 (MQ=255) gAGGAACGACTGTTGGGCATAT‑CCAGCGGAGGGCCCCAGTCCCCCAATAAGCGTCAGCAACCATCAAA‑AAATGCCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACGCCATCAg < 1:81844/150‑1 (MQ=255) gACATTTTGGCGTATACCATC‑GAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCAT‑AAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACgg > 2:132885/1‑151 (MQ=255) ccATC‑AAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCAA‑AAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGAAACAGAAAAACACCAGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGAATTAAGCCCACttt > 1:1385/1‑151 (MQ=255) cgagccccccAGTCCCCAGATAATCGTCCCCACCCATCCAA‑AAATGACAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACttt < 1:377751/141‑1 (MQ=255) cGATAATCGTCACCAACCATCCAT‑AAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCGGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATcc > 1:168516/1‑146 (MQ=255) aTAATCGTCACCAACCATCCAT‑AAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGAATCAGAATCCGAAGAc > 1:305996/1‑150 (MQ=255) aaccGTCACCAACCATCAAT‑AAATGGAAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACa < 2:67684/146‑1 (MQ=255) aaTCGCCACCACCCACCCAA‑AAATGACAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGa < 2:259562/147‑1 (MQ=255) tCACCAACCATCCAT‑AAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTa > 2:33303/1‑150 (MQ=255) tccccgccacccataAAAAGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAAtg < 1:31712/143‑2 (MQ=255) ccatccaa‑AAAGGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTc < 2:310278/148‑1 (MQ=255) t‑AAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGc > 2:172407/1‑151 (MQ=255) | CAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATC‑GAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCAT‑AAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGC > NC_000913/4336751‑4337051 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |