Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,026,941	0	A	C	44.2%	50.1 / 16.6	43	E392D (GAA→GAC)	guaD	guanine deaminase
Reads supporting (aligned to +/- strand):  ref base A (18/6);  new base C (0/19);  total (18/25)
Fisher's exact test for biased strand distribution p-value = 2.28e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGG‑CCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTT‑ACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTA  >  NC_000913/3026795‑3027084                                                                                                                                                     |                                                                                                                                                tccAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGG‑CCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTT‑ACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAAccc                                                                                                                                              >  1:402995/3‑150 (MQ=255)  aCAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGG‑CCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTT‑ACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCAc                                                                                                                                            >  2:304543/1‑151 (MQ=255)   cAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGGTTTACCTGG‑CCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTT‑ACCTGGCAAAGATGCTGATTTCGTGGTGATGGAACCCAcc                                                                                                                                           >  1:113121/1‑151 (MQ=255)                      ggCTATCTCCTCTCGGCATAGGAAGCTTTTTACCTGG‑CCCCGCTCGGCGGGGCAAAATCTCTGGGCCTTGACGTTTTGATTGGCAACTTTTT‑ACCGGGAAAAGAGGCTAATTTCGGGGTGATGGACCCCACCGCCACTCCGCTAcagca                                                                                                                          <  2:387545/149‑1 (MQ=255)                            cGCCTCTCGGCATATGAAGCTTTTTACCTGC‑CCCCGCTCGGGGGAGCAAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTT‑CCCTGGCAAAGAGGCTGATTTCGTGGTGAGGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCt                                                                                                                  <  1:139871/151‑1 (MQ=255)                              cctcgcgcaatagGAGGCTTTTTGCCGGG‑CCCCGCTCGGCGGGGCAAACTCTCGGGGCTTTGCGGATTGAGTTGCCACTTTTTT‑CCCTGGCAAAGAGGTGGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTgc                                                                                                                     <  2:228621/137‑1 (MQ=255)                                  tcGGCATATGAAGCGTTTTACCTGG‑CCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTT‑ACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGAc                                                                                                             >  1:534105/1‑150 (MQ=255)                                      catgtaaaGTGTTTTCCCGGG‑CCACGCCCGGGGGAGCGAATTCTCTGGGCTTTGACTTTTTGATTGGCACCTTTTT‑CCCGGGCAAAGAGGCTGATTTCGTGGTGATGAACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAActc                                                                                                        <  2:505489/145‑1 (MQ=255)                                            acgtgTTTTCCCTTG‑CCCCGCTCGTCGGGGAGAAATCTTTGGGGTTAAACTTTTTGATTGGCACCTTTTC‑CCCTGGCAAAGAGGCTGATTGCGTGGTGATAGACCCCTCCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGtt                                                                                                    <  2:195918/145‑1 (MQ=255)                                             aagcttttACCCGGCCCCCGCCCGCGGGAGCGAATCCTCTGGGCCTTGCCGATTTATTTGGCATCTTTTT‑CCCGGGAAATGAGGCTGTTTTCGTGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGtt                                                                                                    <  1:432507/145‑1 (MQ=255)                                                 ttttTCCGGC‑CCGCGCTCGGCGGAGCAAAGCCGCTGGTCCTTGGCGTTTTGAGTGG‑AACTTTTTGCCCTGCCAAAGAGGCTTTTTCCGTGGGGAGGATCCCCGCCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCttt                                                                                               <  2:611449/149‑1 (MQ=255)                                                  tttCCCTGG‑CCCCGCTGGGCGGAGCAAAATCTCTGGGCCTTGACGTTTTGATTGGCATTTTTTT‑CCCTGGAAAAGAGGCTGATTTCGTGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAg                                                                                             <  1:521316/150‑1 (MQ=255)                                                      ccTGG‑CCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTT‑ACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGAc                                                                                        >  1:154831/1‑151 (MQ=255)                                                            ccACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTT‑ACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAAtt                                                                                   >  1:53991/1‑151 (MQ=255)                                                             cccGCTGGGCGGAGCGAAATCTCTGGGCCTTGATGATTTGATTGGCAACTTTTC‑ACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTg                                                                                  <  2:189942/149‑1 (MQ=255)                                                                gCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTT‑ACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTc                                                                               >  2:381020/1‑151 (MQ=255)                                                                 cTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTT‑ACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCg                                                                              >  1:350369/1‑151 (MQ=255)                                                                     gcggAGCGAAATCTCTGGGCTTTGACTATTTGATGGGCATCTTTTT‑CCCTGGCAAAGGGGCTGATTTCGTGGTGATGAACCCCGCCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCg                                                                              <  2:414483/147‑1 (MQ=255)                                                                       ggAGCGAAATCTCGGGCCCTTGACGATTTGATGGGCACCTTTTT‑CCCGGGAAAAGGGGTTGTTTTGGGGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGtgatg                                                                         <  1:113426/150‑1 (MQ=255)                                                                            gAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTT‑ACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGAc                                                                    >  2:228461/1‑150 (MQ=255)                                                                             aattctcGGGCCCTTGACGATTTGATGGACAACTTTTC‑CCCTGCCAAAGGGGTTGGTTTGGTGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGtgatgatga                                                                     <  2:179775/145‑1 (MQ=255)                                                                              attctcGGGGCCTTGACGTTTTGATTGGCAATTTTTT‑CCCTGGAAAAGGGGCTGTTTTCGTGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGtt                                                                 <  1:82866/149‑1 (MQ=255)                                                                                 ctcgGGGCCTTGACGATTTGATTGGAAACTTTTT‑ACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTACGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGtt                                                                 <  2:443278/148‑1 (MQ=255)                                                                                  tcggggCCTTGACGTTTTGTTTGGCAACTTTTC‑ACCCGGAAAGGACCCTAGTTCGGTGGTGTAGGACCCCTCCGCCACTCCGCTACAGCAGCTGCGATATTACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGAGGACGtt                                                                 <  2:163929/144‑1 (MQ=255)                                                                                    tGGGCCTTGACGATTTGATTGGCAACTTTTT‑CCCTGGAAAAGAGGCTGTTTTCGTGGTGAGGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTggg                                                              <  1:164147/148‑1 (MQ=255)                                                                                    tGGGCCTTGACGATTTGATTGGCAACTTTTT‑ACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCg                                                            >  1:112727/1‑150 (MQ=255)                                                                                     gggCCTTGACGTTTTGATTGGCATTTTTTT‑CCCTGGCAAAGAGGCTGATTTCGTGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTg                                                                <  1:511371/145‑1 (MQ=255)                                                                                     gggCCTTGACGATTTGATGGGCACTTTTTT‑CCCGGGAAAAGAGGTTGTTTTCGTGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGc                                                             <  1:220355/148‑1 (MQ=255)                                                                                      ggCCTTGACGATTTGATTGGCAACTTTTT‑ACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATg                                                         >  1:592131/1‑151 (MQ=255)                                                                                         cTTGACGATTTGATTGGCAACTTTTT‑ACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGAcc                                                      >  1:77803/1‑151 (MQ=255)                                                                                           tGACGATTTGATTGGCAACTTTTT‑ACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGt                                                    >  2:594446/1‑151 (MQ=255)                                                                                                              ttttt‑CCCGGGAAGAGGGGCTGTTTTCGTGGTGAGGAACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCAc                                      <  2:237325/146‑1 (MQ=255)                                                                                                               tttt‑CCCTGGCAAAGAGGCTGATTTCGTGGTGATGAACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTAc                                  <  1:69048/149‑1 (MQ=255)                                                                                                               tttt‑ACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGt                                >  1:612195/1‑151 (MQ=255)                                                                                                                 tt‑ACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTg                              >  1:363731/1‑151 (MQ=255)                                                                                                                    tccTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGAt                            >  2:218197/2‑151 (MQ=255)                                                                                                                              ggggCTGATTTCGTGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTgg                  <  1:195796/149‑1 (MQ=255)                                                                                                                                   tGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTAc             >  1:568012/1‑151 (MQ=255)                                                                                                                                     aTTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACg            >  1:421987/1‑150 (MQ=255)                                                                                                                                      tttCGTGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACg            <  1:113377/149‑1 (MQ=255)                                                                                                                                           tggtgATGAAACCCTCCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCa      <  2:519456/150‑1 (MQ=255)                                                                                                                                            ggtgATGAACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAAc    <  1:606713/151‑1 (MQ=255)                                                                                                                                               gagggAACCCCCCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTGCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGTGCGATGACCGTTCGATCTAACGCACCTACGTTGATGGTCGTCTGGTGCACGAACGCaagtg  <  2:113094/147‑4 (MQ=255)                                                                                                                                                     |                                                                                                                                                TACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGG‑CCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTT‑ACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTA  >  NC_000913/3026795‑3027084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑