Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT41.0% 43.6 / 18.7 40intergenic (‑179/‑28)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (4/19);  new base T (16/0);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.54e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAC  >  NC_000913/3206294‑3206577
                                                                                                                                             |                                                                                                                                                
tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACaaa                                                                                                                                          <  2:3229/150‑1 (MQ=255)
    aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACa                                                                                                                                      >  1:22052/1‑150 (MQ=255)
                ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGGATTTTTTCCGACAaaaaaaaaaaatttag                                                                                                                            >  1:474573/1‑142 (MQ=255)
                  cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGGGTT‑ggc                                                                                                                      >  1:521291/1‑149 (MQ=255)
                  cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAATATAAAAAATGGAg                                                                                                                            >  2:290229/1‑146 (MQ=255)
                           ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAg                                                                                                             <  1:338859/150‑1 (MQ=255)
                               cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAATAAAAAATGGAGGT‑GCCTCGA‑TGAGCg                                                                                                           >  2:607882/1‑148 (MQ=255)
                                aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAGCGaaa                                                                                                        <  2:469501/150‑1 (MQ=255)
                                     tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAATACAAAAAATGGAGTT‑TCCATGA‑TGAGCGaaaaaaa                                                                                                    >  1:528137/1‑145 (MQ=255)
                                     tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAGCGAAAGtaat                                                                                                   <  1:615381/150‑1 (MQ=255)
                                        ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAAAGGAGTT‑GCCATGA‑TTAGGGGAAGgaaaaa                                                                                                 >  1:326457/1‑149 (MQ=255)
                                               tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAGCGGAAGTAATAAacaacaag                                                                                         >  1:151794/1‑148 (MQ=255)
                                                ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATACAAAAAATGGAGGT‑TTCCTGA‑TGAGCGAAAGGAATAAACaa                                                                                             >  1:282677/1‑145 (MQ=255)
                                                  cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAg                                                                                      <  1:176535/150‑1 (MQ=255)
                                                     gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAACGGAGGT‑GGCATGA‑TGAGGGAAAGGAAAAAACAAAAggga                                                                                       >  1:424970/1‑144 (MQ=255)
                                                           tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGt                                                                             <  2:504936/150‑1 (MQ=255)
                                                            cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTT‑GCCATGA‑TGAGCGAAAATAATAAACAACAAGCAGTGAATAAAtt                                                                            >  1:446400/1‑150 (MQ=255)
                                                             aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGa                                                                          <  1:29372/151‑1 (MQ=255)
                                                                 aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACaga                                                                      <  1:73167/151‑1 (MQ=255)
                                                                      tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAtt                                                                  <  2:178795/150‑1 (MQ=255)
                                                                      tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAtt                                                                  <  2:199306/150‑1 (MQ=255)
                                                                                ttCACGGTGGCAACAAATATAAAACCACATTTTTAGAGGTAGTTTGTGGCGGTGAATTTTTACTCCCAAATTATAAAAATGGGGTA‑GCGCTGATTGGGCGAAA‑AAATAAGACAAAGCCACCGGCTAAGGTGTCAGAGATTGTCGCTAAcct                                                       >  2:42374/1‑149 (MQ=255)
                                                                                      gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGc                                                 <  2:273025/151‑1 (MQ=255)
                                                                                        ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa                                               <  1:102253/151‑1 (MQ=255)
                                                                                           aaaaaaTAAAAAACCACATTTTTAGGGGTAGTTTGTGGCGGTGAATTTTCCCAGACAAATAAAAAAACTGGAGTT‑GCCATGT‑TGAGCGAAAGTAATAAGCACCGGGCAGTGAAGAGGTTGACGGGAATTCTCGCAACCTTTACCGCCATg                                             <  2:587147/150‑1 (MQ=255)
                                                                                            aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAATAGAGTT‑TGCATGA‑TGAGGGGAAGTAATAAAAAAAAAGCAGAGAAAAAGGTGAGAAAGAATTTTGCCTACATTACCGCCATg                                             >  2:231918/1‑149 (MQ=255)
                                                                                                atatAAAACCACATTTTTAGTGGTAGTTTGTGGCGGATAAATTTTTTACAAAAATATAAAAAATGGTGTT‑TGGCTGG‑TGAGCGAAAGTAAAAAAAAAAAAGAAGTGAAAAAAGTGAAAGAGAATGTCGCTTAACTTAACGCCATGATTTCt                                       >  2:183448/1‑151 (MQ=255)
                                                                                                    aaaaCCACATTTTTATTTGTAGTTTGGGGCGGTAAATTTTCCCAGACAAAAAAAAAAACTGGAGTT‑GCCATGA‑TGAGCGAAATTAATAAGCAACAGGCAGTAAAAAAGTTCACAGAGTTTGTCCCTACCTTTACCGCCATGATTTCTAccc                                   <  2:465940/151‑1 (MQ=255)
                                                                                                    aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGTT‑GGCATGA‑TGAGGGAAAGGAATAAAAAAAAGGCAGTGAATAAATTGACAGAGATTGTCGCTAACTTTAACGCCATGATTTTTAccc                                   >  1:87197/1‑151 (MQ=255)
                                                                                                     aaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAATTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCg                                  <  1:87389/151‑1 (MQ=255)
                                                                                                         cacaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAACTGGAGTT‑GCCATGA‑TGAGCGAAAAGAATAAACAAAAAGCAGGGAATAAGTTGACAGAGATTGTCGCTAACTTTAACGCCATGATTTCTACCCGaa                                >  1:155689/1‑149 (MQ=255)
                                                                                                         cacaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTT‑TCCCTGA‑TGAGCGCAAGAAATAAACAAAAAGCAGGGAAAAAATTGACAGAGATTGTCGCTAAATTTTACGCCCTGATTTTTACCCCaa                                >  1:544349/1‑149 (MQ=255)
                                                                                                              ttttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTCCCGCCATGATTTCTACCCGAATGCCtg                          <  1:491773/150‑1 (MQ=255)
                                                                                                                 tAGTGGTAGTTTGTGGCGGTGAAATTTTCCACACAAAAAAAAAAACTGGAGTT‑GGCATGA‑TGTGGGAAAGTAAAAAAAAAAAAGCAGTGAATAAAATGACAGAGAATGTCGCTAAAACTAACGCCCTGTTTTTTACCCCAATGTatgatg                       >  2:399956/1‑150 (MQ=255)
                                                                                                                     gttAGTTGGTGGCGGAGAATTTTTCCAAACAAATA‑AAAAAATGGAATTGGCCATAA‑TGTGCGAAATAAATAAGAAAAAGTGATTAATAAATTTGAAAGGGATTGGTGCGAACTTTACCGCCATGATTTCTACCCGAATGCCtgctga                      <  2:168521/145‑1 (MQ=255)
                                                                                                                     ggTAGTTTGGGCCGTTGAATTTTTCCAGACAAATAAAAAAACTGGAGTG‑CCCATGA‑TGAGCGAAAGTAATAAGCAACAGTCAGAAAATAAGTTAACAGATATTTTCGCTAATTTTGCCGCCATGATTTCTACCCGAATGCCTGATGACgt                   <  2:416603/150‑1 (MQ=255)
                                                                                                                          tttGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtggtg               <  1:262020/149‑1 (MQ=255)
                                                                                                                                  ggTGAATTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa      >  1:127037/1‑150 (MQ=255)
                                                                                                                                     gAATTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc  >  1:367553/1‑151 (MQ=255)
                                                                                                                                       aTTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc  >  2:586092/1‑149 (MQ=255)
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TCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAC  >  NC_000913/3206294‑3206577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑