Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,601,1600GA40.7% 31.8 / 11.0 28F309F (TTC→TTTftsXputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (11/5);  new base A (0/11);  total (11/17)
Fisher's exact test for biased strand distribution p-value = 3.49e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAAT  >  NC_000913/3601017‑3601289
                                                                                                                                               |                                                                                                                                   
acacGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCt                                                                                                                            <  2:270676/151‑1 (MQ=255)
  acGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCtgt                                                                                                                          <  1:48343/151‑1 (MQ=255)
    gCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGc                                                                                                                        <  1:165790/151‑1 (MQ=255)
            tattCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCg                                                                                                                >  1:476500/1‑151 (MQ=255)
                  ggCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAAccg                                                                                                          <  2:76605/151‑1 (MQ=255)
                           tGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGATGAc                                                                                                  >  2:36358/1‑150 (MQ=255)
                             gCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGATGACAAt                                                                                               >  1:145184/1‑151 (MQ=255)
                                      ttgtACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCAcc                                                                                      >  1:513618/1‑151 (MQ=255)
                                         caCCGTGGCAACCCACCCCGCCCCCCACCAAACCATAGAGAAGACCAGCACCAACAGCAGGCATTCACCAAATAAAAACCCTTTAATATAAACCTTCTTCCCAAAACCCTGTCCCACTCCCGAAACCGCCGATGACAATCGCAGCACCa                                                                                     <  2:341175/148‑1 (MQ=255)
                                             gcggaaacccACGCCCCCCCCCAGCAAATCAGCGAGCAAACCGGCAGCAATAGAGGCCATCCAACAAAAAAAAACCAATTGATACAAAACTCCTTCCCAAAACCCTGTTCCTCTCCCGAAACCGCCGATGACAATCGCAGCACCAGAAtt                                                                                <  2:579372/142‑1 (MQ=255)
                                             gTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAAttt                                                                               >  1:270316/1‑151 (MQ=255)
                                                     cccccctcccccccacccaATCACCAAGCATCCCAGAAGCAATAGCAGGTATCCATAGAATGATAACCTTTTAATAAAAACCTTCTTCCAAAAACCCTGTCCCACTCCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACa                                                                         <  1:122201/138‑1 (MQ=255)
                                                        cgcccccccccACCAAATCATCGACCAAACCAGCAAAAATAGAAGCCATCCACAGAATAAAAACCATTTAAAAAAAACCTTCTTCCAAAAACCCGGTCCCCCTCCAACATCCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAAt                                                                     <  2:350399/142‑1 (MQ=255)
                                                                   aGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGAcaacaa                                                         <  2:372136/151‑1 (MQ=255)
                                                                     caaacaatcGACCATCCCAACAGAAATAGCAGGCATTCATCAAATGAAAACCCATTAATATCAACCTCCTTCCCAAAACCCTGTCCCCCTACCAAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGacaaca                                                          <  2:139356/142‑1 (MQ=255)
                                                                          cacagAGCATACCGGCAGAAATAGAATCCATTAAGCGATTAAAAAGCCGTGAATAACAACCTCCGTCGCAAAACCATGGTCCTGTCCCGAAACCGCCGATGACAATCGCAGCACCATAATTTCTGACAAAATTAATGacaaca                                                          <  2:464369/139‑1 (MQ=255)
                                                                                    aCCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATccc                                         >  2:353949/1‑150 (MQ=255)
                                                                                          aaaaaacgcggccATTCACCGAATGAAAACCCATTGAAATCAAACTTCTTCCCAAAACCCTGTCCCACTCCAAAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCcagcag                                    <  2:45591/137‑1 (MQ=255)
                                                                                          aaaaaTAGCAGCCTTTCATCGAATAAAAACCCATTAATAACAACCTTCGTCCCAAAACCCTGTCCCCCTCCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCcagcag                                    <  1:171421/146‑1 (MQ=255)
                                                                                                      cATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATAc                       >  1:36478/1‑150 (MQ=255)
                                                                                                       aTTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATAc                       >  2:509922/1‑149 (MQ=255)
                                                                                                            tCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACg                >  2:260409/1‑151 (MQ=255)
                                                                                                               aaagaaaaGCCATTAAAAACAACCTCCGTTCCAAAACCCTGTCCCACTCCCGAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGaa                  <  1:433092/140‑1 (MQ=255)
                                                                                                                 tGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGgcgc           >  1:111229/1‑151 (MQ=255)
                                                                                                                     aaccatttaatctaaaaCTTCGTCCCAAACCCCTGCCCCACTCCCCAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGa       <  1:315770/137‑1 (MQ=255)
                                                                                                                     aaGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAg         >  1:418150/1‑149 (MQ=255)
                                                                                                                           ttaataaCAAACTCCTTCCCAAAACCCTGCCCCCCTCCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAAt  <  1:43192/147‑1 (MQ=255)
                                                                                                                            tctatacAAAATTCATTCACAAAACCGTGCCCCACTCCAACAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGa    <  2:523650/142‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                   
ACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAAT  >  NC_000913/3601017‑3601289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: