Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,665,7020CA42.1% 45.9 / 17.8 38A37S (GCG→TCG) gadXacid resistance regulon transcriptional activator; autoactivator
Reads supporting (aligned to +/- strand):  ref base C (20/2);  new base A (0/16);  total (20/18)
Fisher's exact test for biased strand distribution p-value = 6.88e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATT  >  NC_000913/3665552‑3665838
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aGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGc                                                                                                                                          >  2:102342/1‑151 (MQ=255)
  tgatgaACGAGTGATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACAATTATCTACTCGAATTTGGCTTGCATCCGCa                                                                                                                                         >  2:432072/1‑150 (MQ=255)
      gaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCaaaaa                                                                                                                                     <  1:330621/150‑1 (MQ=255)
           aGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGt                                                                                                                               >  2:622734/1‑151 (MQ=255)
             tAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTc                                                                                                                              >  1:58394/1‑150 (MQ=255)
                   agaaACTTTTTTATGTGCATGGAGATTTAATGCCTCCTCCTTGAGCGTGGGGAGAAATAATGAAAGCGTGTCCCTTGACAAGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGcc                                                                                                                        >  2:377729/1‑150 (MQ=255)
                       aCTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTa                                                                                                                    >  2:30607/1‑150 (MQ=255)
                             ttATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAg                                                                                                              >  2:570486/1‑150 (MQ=255)
                                 gtgCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGat                                                                                                          >  2:515253/1‑150 (MQ=255)
                                       gAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCAcc                                                                                                   >  2:593906/1‑151 (MQ=255)
                                            tttAATCCCTCCTCCTTGAGCAGCGGAAAAAAAAATAAAAGCGTGTCCCTTGACACAAATAAAAAATTTCC‑ACCACACTTTTCTACTAAAATTTGCCTGACACCCAAAAAACCCAGGCAACCGCCATTAAAATAGCGATATTCACCATTa                                                                                               <  2:261833/150‑1 (MQ=255)
                                              tAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                            >  1:317730/1‑151 (MQ=255)
                                                  gCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATgg                                                                                        >  2:547095/1‑151 (MQ=255)
                                                    ctcctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                      >  1:617923/1‑151 (MQ=255)
                                                    cccctcttAGACCATCGAGAAAAATAATGAAGGCGTGCCCTTTGACACGAAT‑CAAAATTTTCAAACACATTTATCTTCTCAAATTTGGCTTGCACCCCAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                      <  2:39787/149‑1 (MQ=255)
                                                      cctcctTGAGCATCGGAAAAAAAAATGAAGGCGTTCCCCTTGACACAAAAACAAAATTTTC‑ACCACACTAATCTACTCAAATTTGGCTTGCTCCCGAAAAACCCAGGGCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTgag                                                                                    <  2:337864/151‑1 (MQ=255)
                                                        tcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTgaga                                                                                   >  2:75020/1‑150 (MQ=255)
                                                         ccttggcaagcGGGAGAAAAAATGAAACCGTGTCCTTTGACACGAATAAAAAATTTCA‑AACACCCTTATCTACTAAAATTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                      <  2:395288/136‑1 (MQ=255)
                                                           ttGAGCACGGGGAAAAATAATGAAAGCGTTCCCTTTGACACAAATACAAAATTTTA‑AACCCCCTTATCTCCTCGAATTGGGCTGCCACCCGAAAAACCCGGGTCTCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                  <  1:392120/148‑1 (MQ=255)
                                                                         aaaTAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGc                                                                  >  2:123523/1‑150 (MQ=255)
                                                                               tGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGc                                                            >  2:438222/1‑150 (MQ=255)
                                                                                 aaaGCGTGTCCTTTGACACAAATAAAAAATTTCA‑AACACACTTATCTACTCGATTTTGGTTTCCACCCGAAAAACCCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCa                                                           <  1:602592/149‑1 (MQ=255)
                                                                                  aaGCGTGTCCCTTGACACGAATAAAAAATTTTC‑AACACCCTTATCTCCTCAATTTTGGCTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAtt                                                        <  1:188878/151‑1 (MQ=255)
                                                                                                       tACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGAt                                   >  1:287858/1‑151 (MQ=255)
                                                                                                          aaaaTTTTC‑ACCACTCTTATCTACTCAAATTTGGCTTACACCCAAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTg                                 <  2:52712/150‑1 (MQ=255)
                                                                                                          aaaaTTTTC‑AACCCTCTTATCTCCGCGATTTTGGCTTCCTTCCCAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGc                                <  1:365230/151‑1 (MQ=255)
                                                                                                          aaaaTTTCC‑AACACACTTATCTCCTAGAATTTGTCTTCCACCCGAAAAACCCAGTTATCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGa                                    <  2:399319/147‑1 (MQ=255)
                                                                                                            aaTTTTC‑AACACACTTATCTACCCGATTTGGGCTTGCATCCGCAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                              <  1:364532/151‑1 (MQ=255)
                                                                                                             aTTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                              >  1:556611/1‑150 (MQ=255)
                                                                                                                 tC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTg                         >  1:104810/1‑151 (MQ=255)
                                                                                                                    aaCACTCTTTTCTCCTCGAATTTGTCTTACATCCGAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATa                             <  1:378388/145‑1 (MQ=255)
                                                                                                                         atttctCTACTCAATTTTGGTTTGCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTa                    <  2:261096/144‑1 (MQ=255)
                                                                                                                           ttATCTACTCGATTTTGGTTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                   <  1:271170/148‑1 (MQ=255)
                                                                                                                              tCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTAc             >  2:351951/1‑151 (MQ=255)
                                                                                                                               ctaccaaattttGGTTTCCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                <  2:155957/138‑1 (MQ=255)
                                                                                                                               cTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTAc             >  1:119141/1‑150 (MQ=255)
                                                                                                                                         ttGTCTTGCATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACatat   <  2:358441/150‑1 (MQ=255)
                                                                                                                                         tgggCTTGAATCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATaa         <  2:12076/142‑1 (MQ=255)
                                                                                                                                          tGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATAtt  >  1:87571/1‑150 (MQ=255)
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AGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATT  >  NC_000913/3665552‑3665838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑