Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,347,104	G→T	C603* (TGC→TGA)	rnb ←	ribonuclease II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,347,104	0	G	T	100.0%	147.3 / NA	40	C603* (TGC→TGA)	rnb	ribonuclease II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/23);  total (17/23)

GAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTGCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGAACACGCATGCCGCCACGGCTGATATCGACAATTTCCGCTGCGAAACGGGTGTCGGTCCCGGCTTTGTCT  >  NC_000913/1346969‑1347248                                                                                                                                        |                                                                                                                                                 gAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATcg                                                                                                                                   >  1:535159/1‑151 (MQ=255)    aGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGTAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCgcgc                                                                                                                                <  2:148547/151‑1 (MQ=255)     ggAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCGGTTCATCGCGca                                                                                                                               <  2:521306/151‑1 (MQ=255)     ggAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGca                                                                                                                               <  2:299023/151‑1 (MQ=255)          ttACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCg                                                                                                                          >  2:31645/1‑151 (MQ=255)            aCGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCgtg                                                                                                                        <  2:306136/151‑1 (MQ=255)                   cGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGaa                                                                                                                  >  2:553556/1‑150 (MQ=255)                    gggCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAg                                                                                                                <  2:114167/151‑1 (MQ=255)                           cAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCgg                                                                                                         <  2:75836/151‑1 (MQ=255)                                atGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATaa                                                                                                    <  2:349823/151‑1 (MQ=255)                                  gctgcGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATaaa                                                                                                   <  2:280170/150‑1 (MQ=255)                                      cGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCg                                                                                               >  1:181084/1‑150 (MQ=255)                                              cATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCg                                                                                      <  1:620912/151‑1 (MQ=255)                                               aTGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGt                                                                                     <  1:464073/151‑1 (MQ=255)                                               aTGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGt                                                                                     <  1:493337/151‑1 (MQ=255)                                               aTGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGt                                                                                     <  1:279837/151‑1 (MQ=255)                                                tGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGtt                                                                                    >  1:282597/1‑151 (MQ=255)                                                 gCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTa                                                                                   <  2:385931/151‑1 (MQ=255)                                                        tCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAAcc                                                                             <  2:555185/150‑1 (MQ=255)                                                         cGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCa                                                                            <  1:328750/150‑1 (MQ=255)                                                               tAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGAac                                                                     >  1:368539/1‑151 (MQ=255)                                                                aGTGACGTCAATAACATCAGTTACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTGTCAACCAAACGaa                                                                      >  2:395875/1‑149 (MQ=255)                                                                    aCGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGAACACGCa                                                                <  1:106597/151‑1 (MQ=255)                                                                       tCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGAACACGCATgc                                                             >  1:291890/1‑151 (MQ=255)                                                                           tAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGAAAGAACGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGACCACGCATgccgc                                                          >  2:142795/1‑150 (MQ=255)                                                                               gtcagtcaCTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGAACACGCATGCCGCCACgg                                                     >  1:597431/1‑151 (MQ=255)                                                                                     caCTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGAACACGCATGCCGCCACGGCTGat                                                <  1:528736/150‑1 (MQ=255)                                                                                      aCTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGAACACGCATGCCGCCACGGCTGatat                                              >  2:604457/1‑151 (MQ=255)                                                                                         ttGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGAACACGCATGCCGCCACGGCTGATATCg                                            <  2:527682/150‑1 (MQ=255)                                                                                                         cTTTAATTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGAACACGCATGCCGCCACGGCTGATATCGACAATTTCCGCTGCGa                            >  2:472217/1‑150 (MQ=255)                                                                                                             aaTTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGAACACGCATGCCGCCACGGCTGATATCGACAATTTCCGCTGCGAAACgg                       >  1:237298/1‑151 (MQ=255)                                                                                                             aaTTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGAACACGCATGCCGCCACGGCTGATATCGACAATTTCCGCTGCGAAACgg                       >  1:9671/1‑151 (MQ=255)                                                                                                              aTTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGAACACGCATGCCGCCACGGCTGATATCGACAATTTCCGCTGCGAAACggg                      <  1:523606/151‑1 (MQ=255)                                                                                                              aTTTGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGAACACGCATGCCGCCACGGCTGATATCGACAATTTCCGCTGCGAAACggg                      <  1:31654/151‑1 (MQ=255)                                                                                                                 tGTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGAACACGCATGCCGCCACGGCTGATATCGACAATTTCCGCTGCGAAACGGgtg                    <  1:357664/150‑1 (MQ=255)                                                                                                                  gTACAGTGCCGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGAACACGCATGCCGCCACGGCTGATATCGACAATTTCCGCTGCGAAACGGGTGTc                  <  2:234123/151‑1 (MQ=255)                                                                                                                   tACAGTGCCGTTTTCCTGGCTTCAAACCAGCTCATCGCGCACAGCGTGTACGAAAGGGGCCGGAATAAAGCCGATAGCGCCGTTATCAACCAAACGAACACGCATGCCGCCACGGCTGATATCGACAATTTCCGCTGCGAAACGGGTGTCg                 >  2:147971/1‑151 (MQ=255)                                                                                                                           cGTTTTCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGGGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGAACACGCATGCCGCCACAGCTGATATCGACAATTTCCGCTGCGAAACGGGTGTCGGTCCCGGc         >  2:106476/1‑151 (MQ=255)                                                                                                                               ttCCTGGCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGAACACGCATGCCGCCACGGCTGATATCGACAATTTCCGCTGCGAAACGGGTGTCGGTCCCGGCTTTg     >  1:123701/1‑151 (MQ=255)                                                                                                                                    ggCTTCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGAACACGCATGCCGCCACGGCTGATATCGACAATTTCCGCTGCGAAACGGGTGTCGGTCCCGGCTTTGTCt  <  2:222261/149‑1 (MQ=255)                                                                                                                                        |                                                                                                                                                 GAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTGCAAACCAGTTCATCGCGCACAGCGTGTAAGAAAGGTGCCGGAATAAAGGCGATAGCGCCGTTATCAACCAAACGAACACGCATGCCGCCACGGCTGATATCGACAATTTCCGCTGCGAAACGGGTGTCGGTCCCGGCTTTGTCT  >  NC_000913/1346969‑1347248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑