Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,630,841	C→A	V25F (GTT→TTT)	ydfI ←	putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,630,841	0	C	A	100.0%	130.1 / NA	34	V25F (GTT→TTT)	ydfI	putative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/18);  total (16/18)

TATCTTGCTGTTGTAAATCGGCAATTTGCTGTTCGCCGCCGATTAAGTTGACCTCATAATATCCCCAGTCACTGAAATGTTCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAACAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAG  >  NC_000913/1630694‑1630985                                                                                                                                                    |                                                                                                                                                 tATCTTGCTGTTGTAAATCGGCAATTTGCTGTTCGCCGCCGATTAAGTTGACCTCATAATATCCCCAGTCACTGAAATGTTCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAaaaa                                                                                                                                                <  2:98763/150‑1 (MQ=255)        cTGTTGTAAATCGGCAATTTGCTGTTCGCCGCCGATTAAGTTGACCTCATAATATCCCCAGTCACTGAAATGTTCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTgg                                                                                                                                         >  2:319921/1‑150 (MQ=255)          gttgtAAATCGGCAATTTGCTGTTCGCCGCCGATTAAGTTGACCTCATAATATCCCCAGTCACTGAAATGTTCCGTAGCAAGAATATCGGCACACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAg                                                                                                                                       <  2:32149/150‑1 (MQ=255)             gtAAATCGGCAATTTGCTGTTCGCCGCCGATTAAGTTGACCTCATAATATCCCCAGTCACTGAAATGTTCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCa                                                                                                                                    <  1:198464/150‑1 (MQ=255)              tAAATCGGCAATTTGCTGTTCGCCGCCGATTAAGTTGACCTCATAATATCCCCAGTCACTGAAATGTTCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAgg                                                                                                                                  >  2:78885/1‑151 (MQ=255)                       aaTTTGCTGTTCGCCGCCGATTAAGTTGACCTCATAATATCCCCAGTCACTGAAATGTTCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGttatta                                                                                                                            >  2:418518/1‑148 (MQ=255)                            gCTGTTCGCCGCCGATTAAGTTGACCTCATAATATCCCCAGTCACTGAAATGTTCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCAt                                                                                                                     >  2:259621/1‑150 (MQ=255)                               gTTCGCCGCCGATTAAGTTGACCTCATAATATCCCCAGTCACTGAAATGTTCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAAc                                                                                                                 <  1:303756/151‑1 (MQ=255)                                    ccgccgATTAAGTTGACCTCATAATATCCCCAGTCACTGAAATGTTCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGa                                                                                                            <  1:465429/151‑1 (MQ=255)                                     cgccgATTAAGTTGACCTCATAATATCCCCAGTCACTGAAATGTTCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAggg                                                                                                             >  1:32380/1‑149 (MQ=255)                                        cgATTAAGTTGACCTCATAATATCCCCAGTCACTGAAATGTTCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAgtgt                                                                                                        >  1:407133/1‑151 (MQ=255)                                                             atCCCCAGTCACTGAAATGTTCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAatt                                                                                   <  2:92447/151‑1 (MQ=255)                                                             atCCCCAGTCACTGAAATGTTCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAatt                                                                                   >  1:532447/1‑151 (MQ=255)                                                               ccccAGTCACTGAAATGTTCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAatta                                                                                  <  1:398393/150‑1 (MQ=255)                                                                      cACTGAAATGTTCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCa                                                                           <  1:479386/150‑1 (MQ=255)                                                                       aCTGAAATGTTCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATa                                                                         <  1:47184/151‑1 (MQ=255)                                                                       aCTGAAATGTTCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATa                                                                         <  1:275734/151‑1 (MQ=255)                                                                              tGTTCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTc                                                                  >  1:305863/1‑151 (MQ=255)                                                                                ttCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAATATCATAAGCAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTcc                                                                 <  2:388600/150‑1 (MQ=255)                                                                                 tCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCtt                                                               >  1:419400/1‑151 (MQ=255)                                                                                  ccGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCtt                                                               >  1:269877/1‑150 (MQ=255)                                                                                       gCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTtaaatat                                                         <  1:418657/151‑1 (MQ=255)                                                                                         aaGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTtaaatataa                                                       <  2:468188/151‑1 (MQ=255)                                                                                             atatCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTaaatataaatat                                                   <  2:574824/151‑1 (MQ=255)                                                                                              tatCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATg                                                  >  2:165761/1‑151 (MQ=255)                                                                                                tCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGc                                                >  2:268226/1‑151 (MQ=255)                                                                                                 cGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGc                                                <  2:76955/150‑1 (MQ=255)                                                                                                                 aTGCGCACGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGtt                               <  1:396077/151‑1 (MQ=255)                                                                                                                        cGGTGAAATGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGaa                        <  1:86854/151‑1 (MQ=255)                                                                                                                              aaTGCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATaaaaa                  <  2:603173/151‑1 (MQ=255)                                                                                                                                 gCACCAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATc                <  1:467577/150‑1 (MQ=255)                                                                                                                                    ccAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATcccc             >  2:448044/1‑150 (MQ=255)                                                                                                                                    ccAAAGCCTAAATGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCct            >  2:535018/1‑151 (MQ=255)                                                                                                                                     cAAAGCCTAAATGAAAACTTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCct            >  2:488947/1‑150 (MQ=255)                                                                                                                                                tGAAAAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAag  >  1:583161/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                 TATCTTGCTGTTGTAAATCGGCAATTTGCTGTTCGCCGCCGATTAAGTTGACCTCATAATATCCCCAGTCACTGAAATGTTCCGTAGCAAGAATATCGGCATACACACCCTGATGCGCACGGTGAAATGCACCAAAGCCTAAATGAACAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAG  >  NC_000913/1630694‑1630985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑