| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,151,686 | 0 | G | T | 41.0% | 8.2 / 16.0 | 39 | intergenic (+40/+25) | yegJ/yegK | uncharacterized protein/ser/thr phosphatase‑related protein |
| Reads supporting (aligned to +/- strand): ref base G (9/14); new base T (2/14); total (11/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.57e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.09e-01 | |||||||||||
GATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTG‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCG > NC_000913/2151538‑2151826 | gATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAttgtt < 2:471233/151‑1 (MQ=255)gATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAttgtt < 2:33665/151‑1 (MQ=255) atccatttGTTCTTTTATTGGTCCCCTACCAAAAAAAAGACGCCGACGAGTTAAGAAAAAAATTAGTTCCCAAATCAGCCGTTATATTTTTTGTTAAACCGGGCGTTTAAATTTTTG‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCca < 2:286629/144‑1 (MQ=255) taCCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTGATTGTTG‑T‑CTTCAACCGCCATGAGAAAATCACACTTGCcac > 1:598170/1‑150 (MQ=255) cATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGCGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTAATTTTTG‑T‑CTTTAAACGCCCACAGAAACTCACTCTGc > 1:945042/1‑143 (MQ=255) ttGATCCTTTATTGGTCCCCTACCCAAAAGAAGAGCCCGACGAGCTAAGAGAAAAATTAGTTCGCTAATCACCCGTTATATTTATTTTTTAAACGGGGGTTTGAATTGTTG‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAgcg < 2:633029/149‑1 (MQ=255) ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTG‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCa > 2:451358/1‑150 (MQ=255) ttGTTCCCCTATCAAAAAGAAGAAGCCGACGAGCAAAGAAAAAAATTATTTCTCTAATCAGCCGTTATATTTATTTTTAAACCGGGCGGTTAATTTGTTG‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACg < 1:560588/150‑1 (MQ=255) ccccTATCAAAAAGAAGAAGCCGACGAGCTAAGAGAAAAATTAGTTCGCTAATCAGCCTTTATATTTATTGTTAAAACGGGCGGTTGATTTGTTG‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTa < 2:665254/148‑1 (MQ=255) ccTACCCAAAAGAAGAAGCCGACGAGCAAAGAGAAAAATTAGTTCTCTAATCAGCCTTTATATTTATTGTTTAACCTGGCGTTTGATTTGTTG‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatca < 2:974386/151‑1 (MQ=255) tATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTG‑T‑CTTTAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGGGCCAACGtttt > 1:305485/1‑145 (MQ=255) gccaaaaaGAAAAACCCATCGAGATAAGAAAAAAATATTTTCGCTAATCACCTTTTATATTTATGTATTAAACTGGCGGTTAAATTTTTT‑T‑TTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatca < 1:236685/144‑1 (MQ=255) aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTG‑T‑CTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGGGCCCACGTTTTatcat > 1:853031/1‑149 (MQ=255) aaaaaaAGAAGCCGACGAGCAAAGAGAAAAATTAGTTCGCTAATCAGCCGTTATATTTATTTTTAAAACGGGCGGTTGAATTGTTG‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcat < 2:985247/145‑1 (MQ=255) aagaagaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTG‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGt > 2:1089512/1‑150 (MQ=255) gaGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTG‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCCGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTAccg > 2:874509/1‑150 (MQ=255) aGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTG‑T‑CTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTAccgcc > 1:355513/1‑151 (MQ=255) gcaaaGAGAAAATTTATTTGGCAAACCCGCCTTTATTTTTTTTTTTAAACCGGGCGTTTAATTTTTTG‑T‑CTTCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCACCCGTACGCTCGTTTAccgccg < 2:1055621/148‑1 (MQ=255) aaGAGAAAAATTGTTTCCTTAATCACCCTTTATTTTTATT‑TTTAAACCTGGGGTTTAATTTTTT‑TCCTTCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGc < 1:915903/151‑1 (MQ=255) agaaaaaaaTTAGTTCTTAAATCCGCTTTTATTTTTTTTGTTTAAACGGGCGGTGTATTTTTGG‑T‑TTCCAGCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCt < 1:294200/146‑1 (MQ=255) agCAAAATTTGTTCGCTAAGCCGCCTTTATTTGTTTTTTTAAAACTGGGGGTTAATTTTTTG‑T‑TTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCgg < 1:438292/146‑1 (MQ=255) atttttgcGCTAATCCGCCTTTATATTTATTGTAAAAACGGGGGTTTGAATTGTTT‑T‑TTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaaaa < 1:379342/144‑1 (MQ=255) ttCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTG‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGGTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaaaaa > 2:553323/1‑147 (MQ=255) aTCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTG‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCtttta > 2:665224/1‑147 (MQ=255) ccGTTATATTTATTGTTAAATCGGGCGGTTTATTTTTTG‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCa < 1:465733/151‑1 (MQ=255) gTTATTTTTTTTGTTTAAACTGGGGTTTAAATTGTTG‑T‑TTAAAACCGCCATGCGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTc < 2:260865/148‑1 (MQ=255) ttATTTTTTTTTTTAAACCGGGCGGTTGAATTGTTG‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCtt < 2:125911/138‑1 (MQ=255) ttATATTTATTGTTAAACCTGGCGTTTGAA‑TGTTGTT‑CTCCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAgg < 1:485790/150‑1 (MQ=255) tataTTTATTGTTTAAACTGGCGGTTGAATTGTTG‑T‑CTTCTACCTCCATTAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTAACGCCGGGCTGGACAAAAACTGCTTTaaaa > 1:5670/1‑140 (MQ=255) atatTTATTTTTTAACCTGGGGGTTGATTTTTTG‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGta < 2:684541/150‑1 (MQ=255) tttttattGTATAACCGGGCGGTTAATTTTTTT‑T‑CTCCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAgg < 1:554429/145‑1 (MQ=255) tatttattTTTTAACCGGGCGTTTGAATTGTTG‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGta < 2:465672/149‑1 (MQ=255) ttttttttttAAACTGGGGGTTAATTTGTTT‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGta < 2:775312/147‑1 (MQ=255) ttttttttaaaaccgggCGTTTAATTTGTTG‑T‑CTCAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGtaata < 1:916243/135‑1 (MQ=255) tttattTTTTAATCTGGCGTTTAATTTTTTC‑T‑TTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGtaata < 2:139171/150‑1 (MQ=255) tttttttttAAAGTGGCGGTTAATTTTTTG‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGtaataa < 1:769823/144‑1 (MQ=255) ttttttAACCGGGCGGTTAATTTTTTT‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAAt < 1:589918/146‑1 (MQ=255) ttGTTTAACCTGGCGTTTAATTTTTTG‑T‑TTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATc < 1:921017/150‑1 (MQ=255) ttGTTAAAACGGGGGGTTGATTTGTTG‑T‑TTTCGACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATc < 2:267204/150‑1 (MQ=255) gCGGTTAAATTGTTT‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGc < 2:359070/151‑1 (MQ=255) ggTTGAATTTTTT‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCg < 2:700197/150‑1 (MQ=255) | GATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTG‑T‑CTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCG > NC_000913/2151538‑2151826 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |