Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	42.1%	85.2 / 19.3	57	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (4/29);  new base G (24/0);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 2.72e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGC  >  NC_000913/354141‑354430                                                                                                                                                     |                                                                                                                                                ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTg                                                                                                                                                <  2:349807/149‑1 (MQ=255) ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGa                                                                                                                                               <  1:1071500/150‑1 (MQ=255) ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGa                                                                                                                                               <  1:175141/150‑1 (MQ=255)       tCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTTGGGGGAAAg                                                                                                                                            >  2:676086/1‑147 (MQ=255)            gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTg                                                                                                                                    <  2:1101936/150‑1 (MQ=255)            gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑a                                                                                                                                  <  2:738537/151‑1 (MQ=255)                         aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAAATGCGGTG‑AAAGGGAAggggg                                                                                                                      >  2:641727/1‑150 (MQ=255)                              tttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAAAAGCGCCG‑GAAGGGCAggtgggggggg                                                                                                                >  2:30119/1‑151 (MQ=255)                              tttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTTGGGGGGAAGATGCGCTG‑AAAGGGCAggggggggggg                                                                                                                >  1:557149/1‑151 (MQ=255)                                tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGGGGCGGTG‑GAAGGGCAggggggggggggg                                                                                                              >  1:139437/1‑149 (MQ=255)                                 aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGAAGCGCTG‑GAAGGGCCggggggggggggg                                                                                                              >  1:619046/1‑148 (MQ=255)                                    ctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTG‑GAAGGGCAGgtggcggtggcggt                                                                                                            >  1:693823/1‑147 (MQ=255)                                    ctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGGCGGGCTG‑GGAGGGGAggggggggggggggtt                                                                                                           >  1:184095/1‑145 (MQ=255)                                           gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTcc                                                                                                   <  2:858841/151‑1 (MQ=255)                                           gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCAAGCCATCCGGGCGTTGCCGAAGGGGCGGGGGGTGGGGGGGGAAGCGGGGTGG‑AAAGGG‑AGGGGGCGGTGGGGATTGTCCtttc                                                                                                   >  1:919555/1‑149 (MQ=255)                                               tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTG‑AAAGGGCAGGGGGGGGTGGGGGTTGTCATTCCGaa                                                                                                >  2:650674/1‑150 (MQ=255)                                               tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTG‑AAAGGGCAGGGGGCGGTGGCGGTTGTCATTCCGaa                                                                                                >  2:68587/1‑150 (MQ=255)                                                gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaa                                                                                                <  2:653026/149‑1 (MQ=255)                                                gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaa                                                                                                <  2:255310/149‑1 (MQ=255)                                                gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAag                                                                                              <  1:160120/151‑1 (MQ=255)                                                    aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAagaggg                                                                                          <  2:862181/151‑3 (MQ=255)                                                    aTGTAATTAACGTTGACGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAagaga                                                                                           <  2:20649/150‑1 (MQ=255)                                                      gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGc                                                                                         <  2:243674/150‑1 (MQ=255)                                                              cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTg                                                                                <  2:987152/151‑1 (MQ=255)                                                              cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGGGCTG‑AAAGGGCAGGGGGGGGGGGCGTTTGTCATTCCGAAAAAGAGCGACAGTc                                                                                  >  2:511060/1‑149 (MQ=255)                                                                 tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAGATGCGCTG‑AAAGGGGAGGGGGCGGGGGGGGTTTTCATTCCGAAAGAGAGCGACAGTCTGGa                                                                              >  2:1037950/1‑150 (MQ=255)                                                                   ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTG‑AAAGGGGAGGGGGCGGTGGGGTTTGTCATTTCGAAAGAGAGCGACAGTCTGGa                                                                              >  2:806116/1‑148 (MQ=255)                                                                   ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTG‑AAAGGGGAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGGCTgg                                                                               >  1:596708/1‑147 (MQ=255)                                                                    cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                                                                          <  2:537834/151‑1 (MQ=255)                                                                    cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                                                                          <  1:57774/151‑1 (MQ=255)                                                                    cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGGGGGGGGGAAAATGCGCTG‑AAAGGGCGGGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGGGACAGTCTgg                                                                               >  1:415701/1‑146 (MQ=255)                                                                        cATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTTGGGGGGAAA‑AAGAGCTGTAAGGGGCAGG‑GGGGGGGGGGGTTGGCATTCCGAAAGAGGGCGGCAGTCTGGAAGagtgtg                                                                      >  1:835885/1‑146 (MQ=255)                                                                               tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAAATGCGCCG‑GAAGGGCGGGGGGGGGGGGCGTTTGTGCTATCGTAAGAGAGCGAAAGTGTGGGAGAACGGGATGTgg                                                                >  1:597423/1‑150 (MQ=255)                                                                               tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGCGGGG‑AAAGGGCAGGGGGCGGGGGCGCTTGTCTTTCCGAAAGAGAGCGACCGGCTGGGAGAACGTGGTGTgg                                                                >  2:432182/1‑150 (MQ=255)                                                                                ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTG‑AAAGGGCCGGGGAGGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGGTGTGgcg                                                              >  1:251724/1‑151 (MQ=255)                                                                                   gACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTTGGTGGGGGGGAAGGGGAGCTG‑AGAGGGGACGAGGCCGCGGCGTTTGTCTTTCCGAAAGAGGGCGAAAAACTGGAAGAtttcggtatggcgcgc                                                           >  2:1032930/1‑136 (MQ=255)                                                                                        gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCa                                                      <  1:363932/151‑1 (MQ=255)                                                                                          gagaTTGGAGAGAGTATCTCCAGGCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGTAGGTGGCGGTGGCGTTTGTCAGGCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACGCGCaa                                                     <  1:868880/150‑1 (MQ=255)                                                                                          gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGAAGGGCTG‑AAAGGGGAGGGGGCGGGGGGGTTTTTCATTCCGGAAAAGAGGGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                         >  2:525633/1‑146 (MQ=255)                                                                                               tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGGAAGATGCGCTG‑AAAGGGCGGGGGGCGGGGGGGGTTGTCATTCCGGAAGAGAGCGACAGTTTGGAAGATTGTGATTTGGCGAACTCGCaa                                                     >  2:303551/1‑145 (MQ=255)                                                                                               tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGaa                                                <  2:558513/150‑1 (MQ=255)                                                                                                gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAgg                                              <  2:254616/151‑1 (MQ=255)                                                                                                gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAg                                               <  1:418559/150‑1 (MQ=255)                                                                                                 aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                             <  2:475891/151‑1 (MQ=255)                                                                                                   gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGa                                           <  2:557251/151‑1 (MQ=255)                                                                                                      agTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGAAGCGCTG‑AAAAGGCAGGGGGGGGGGGGGTTTGTCATTCCGAAAGAGGGCGGCAGACTGGGAGATCGGGGTGTGGGGCACTCGCAAGAGAAGGCGGtt                                         >  1:455962/1‑150 (MQ=255)                                                                                                        tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAAATTCCCTG‑AAAGGGCAGGGGGCGGGGGGGGTTGTCATTTCCAAAAAGGGGGACAGGCTGGGAGATAGGGATGTGGGGCCCCCGCAagag                                                  >  1:273875/1‑139 (MQ=255)                                                                                                         aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTa                                        <  1:204114/148‑1 (MQ=255)                                                                                                                    tCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGTGAAAGATGCCCTG‑GAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGGGGTGTGGGGCGCTCGCAAGAGAAGGCGATTATGGGGCtggggg                            >  1:327174/1‑149 (MQ=255)                                                                                                                          cGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAg                    <  2:1083632/151‑1 (MQ=255)                                                                                                                              gCCGAAGTGGC‑GGTTGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt                 <  1:476734/150‑1 (MQ=255)                                                                                                                                cGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTG‑AAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAAAGAGCGACAGGCTGGGAGATCGGGATGTGGCGCACTCGCCAGAGAAGGCGGTTATGGCGCTGGTGGGCAGCCAGATTgg               >  1:425873/1‑150 (MQ=255)                                                                                                                                     tGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACtt         >  2:1017215/1‑151 (MQ=255)                                                                                                                                      ggC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACttt        <  2:284193/151‑1 (MQ=255)                                                                                                                                      ggC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACttt        <  1:1053543/151‑1 (MQ=255)                                                                                                                                      ggC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACtt         <  1:894058/150‑1 (MQ=255)                                                                                                                                             ggtTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgc  <  2:139467/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGC  >  NC_000913/354141‑354430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑