Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,186,605 A→C H419P (CAC→CCC)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,186,6050AC98.7% 276.8 / NA 79H419P (CAC→CCC) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (38/40);  minor base G (0/1);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCT  >  NC_000913/4186457‑4186750
                                                                                                                                                    |                                                                                                                                                 
aagaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAAcccc                                                                                                                                                 >  2:1024835/1‑151 (MQ=255)
  gaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCg                                                                                                                                               <  1:545470/151‑1 (MQ=255)
        ggCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTAct                                                                                                                                          <  2:737975/150‑1 (MQ=255)
          cACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTActgc                                                                                                                                        >  1:1070368/1‑150 (MQ=255)
           aCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTActgctg                                                                                                                                      <  1:138070/151‑1 (MQ=255)
                      tCAAACCGTTCATCTACGGCAAGCTGTAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCAcc                                                                                                                           >  2:12825/1‑151 (MQ=255)
                             gTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTg                                                                                                                    <  1:379747/151‑1 (MQ=255)
                             gTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTg                                                                                                                    >  2:392681/1‑151 (MQ=255)
                                     aCGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCt                                                                                                            <  2:74169/151‑1 (MQ=255)
                                           aGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTAt                                                                                                      <  2:1047209/151‑1 (MQ=255)
                                            gCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATc                                                                                                     >  1:334295/1‑151 (MQ=255)
                                                    tGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTTAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAtt                                                                                             >  2:721339/1‑151 (MQ=255)
                                                     gCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAtt                                                                                             <  2:770727/150‑1 (MQ=255)
                                                      cGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTg                                                                                           >  2:344901/1‑151 (MQ=255)
                                                       gTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGa                                                                                          <  1:842325/151‑1 (MQ=255)
                                                       gTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGa                                                                                          <  1:49329/151‑1 (MQ=255)
                                                         ggTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAc                                                                                        >  2:719703/1‑151 (MQ=255)
                                                          gTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGaa                                                                                         <  2:305038/149‑1 (MQ=255)
                                                           tCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCg                                                                                      >  1:558173/1‑151 (MQ=255)
                                                           tCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCg                                                                                      >  2:846682/1‑151 (MQ=255)
                                                           tCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCg                                                                                      >  1:1063938/1‑151 (MQ=255)
                                                              tGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCg                                                                                      <  2:762681/148‑1 (MQ=255)
                                                              tGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGt                                                                                    >  2:754802/1‑150 (MQ=255)
                                                              tGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGt                                                                                    <  2:462109/150‑1 (MQ=255)
                                                                  accaccATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTg                                                                                <  1:766733/150‑1 (MQ=255)
                                                                  accaccATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTg                                                                                <  2:1095135/150‑1 (MQ=255)
                                                                   ccaccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGAt                                                                              >  1:765229/1‑151 (MQ=255)
                                                                       caTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGa                                                                           >  1:416531/1‑150 (MQ=255)
                                                                       caTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGCATCCAGGCATTTGAACCGGTACTGATCg                                                                            >  1:348423/1‑149 (MQ=255)
                                                                          tAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAgg                                                                        <  1:506825/150‑1 (MQ=255)
                                                                          tAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAgg                                                                        >  1:767107/1‑150 (MQ=255)
                                                                            aaGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTa                                                                      >  1:43260/1‑150 (MQ=255)
                                                                            aaGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTa                                                                      >  2:508536/1‑150 (MQ=255)
                                                                            aaGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTa                                                                      >  1:11427/1‑150 (MQ=255)
                                                                             aGCTGCGAAGAAAATGGTGGAGCGCGAAGAAGCTGTCGTTTGGGATAGCCTGGACGAAGTTATCCGCGAACCCCCGGTGCTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTaaa                                                                    <  1:270054/151‑1 (MQ=255)
                                                                               cTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGc                                                                  >  1:24956/1‑151 (MQ=255)
                                                                                 gCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCt                                                                 <  2:343521/150‑1 (MQ=255)
                                                                                 gCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCt                                                                 <  1:481871/150‑1 (MQ=255)
                                                                                  cGAAGAAAATGGTTCATCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGCAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTAt                                                               >  1:565983/1‑151 (MQ=255)
                                                                                     agaaAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCa                                                            <  1:723263/151‑1 (MQ=255)
                                                                                        aaaTGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAg                                                           >  2:649282/1‑149 (MQ=255)
                                                                                         aaTGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTg                                                        <  2:125833/151‑1 (MQ=255)
                                                                                          aTGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGc                                                       >  2:866026/1‑151 (MQ=255)
                                                                                               tGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCg                                                  >  2:951834/1‑151 (MQ=255)
                                                                                                 aGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCt                                                <  2:2696/151‑1 (MQ=255)
                                                                                                  gcgcGAAGCAGCTGTCGTGTGGGATATCCTGGACGAAGTTATCCGCGAACGCCCGGTACGGCTGATCCGTGCACCCACTCTGCACCGTCTGGGTATCCGGGCTTTTGACCCGGGACTGATCGAAGATAAAGCTATCCAGCTGCACCCGCt                                                <  1:730334/150‑1 (MQ=255)
                                                                                                   cgcgAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCCCCCGCTgt                                              <  1:929429/151‑2 (MQ=255)
                                                                                                   cgcgAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTgg                                              <  2:633116/151‑1 (MQ=255)
                                                                                                   cgcgAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTgg                                              >  2:674669/1‑151 (MQ=255)
                                                                                                     cgAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGtt                                            <  2:858641/151‑1 (MQ=255)
                                                                                                       aagaagCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTtg                                          <  2:406538/151‑1 (MQ=255)
                                                                                                         gaagCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTtgtg                                        >  2:981183/1‑151 (MQ=255)
                                                                                                           agCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCg                                      <  2:120923/151‑1 (MQ=255)
                                                                                                           agCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCg                                      >  2:493154/1‑151 (MQ=255)
                                                                                                                 cGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCatat                                <  1:1001243/151‑1 (MQ=255)
                                                                                                                 cGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCatat                                <  2:645485/151‑1 (MQ=255)
                                                                                                                 cGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCata                                 <  2:749556/150‑1 (MQ=255)
                                                                                                                 cGTGTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCGCCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCata                                 <  1:323318/150‑1 (MQ=255)
                                                                                                                  gttcgggagagacTGGACCACGTGCTCCTCGAACCCACGGTACTGCTGAACCGGGCGCCGACACGGCACCGTCTGGGTATCCATGCATTTGAACCGGGACGGATCGAATGTAAATCTATCCAGCGTCACCCGCTGGTTTGTGCGGCa                                   <  1:521831/135‑1 (MQ=255)
                                                                                                                  gTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCAtata                               <  2:510738/151‑1 (MQ=255)
                                                                                                                   tttGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCAtata                               >  2:821890/1‑150 (MQ=255)
                                                                                                                    ttGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATaa                              <  1:142654/150‑1 (MQ=255)
                                                                                                                    ttGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATaa                              <  1:42596/150‑1 (MQ=255)
                                                                                                                    ttGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGCCTCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAAc                             >  1:327447/1‑151 (MQ=255)
                                                                                                                    tcggggTATCCTGGACGAAGTTATCCGCGAAACCCCGGCACTGCTGAACCGTGCACCGAGTCTGCACCGTCTGGGTAGCCGGGCATTTGAACCGGCACTGATCGAAGGTAAAGGTATCAAGCGACACCCGCTGGTTTGTGGGGCATATaa                              >  1:750634/3‑150 (MQ=255)
                                                                                                                      gggATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGc                           <  2:125383/151‑1 (MQ=255)
                                                                                                                         atatCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGa                        <  1:1036286/151‑1 (MQ=255)
                                                                                                                         atatCCTGGACGAAGTTATCCGCGAACCCCCGGCACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAACGGTACTGATCGAAGGGAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGcc                          >  1:53934/1‑149 (MQ=255)
                                                                                                                          tatCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGAc                       >  2:936516/1‑151 (MQ=255)
                                                                                                                            tCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACtt                     >  1:373598/1‑151 (MQ=255)
                                                                                                                              cTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCAGCATATAACGCCGACTTCg                   >  1:991427/1‑151 (MQ=255)
                                                                                                                                ggACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCg                   <  2:744885/149‑1 (MQ=255)
                                                                                                                                      aGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGAc           <  1:1024442/151‑1 (MQ=255)
                                                                                                                                         tATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGa            >  1:952712/1‑147 (MQ=255)
                                                                                                                                         tATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCa         <  2:362750/150‑1 (MQ=255)
                                                                                                                                          aTCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGAcc          >  2:660159/1‑148 (MQ=255)
                                                                                                                                           tCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGAcc          >  2:1075246/1‑147 (MQ=255)
                                                                                                                                            ccGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATg     <  1:751111/151‑1 (MQ=255)
                                                                                                                                               tgAACCCCCGGTACCGCTGAACCGTGCACCGACTCTGCACCGTCTGGGCATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGAGACCAGATgg    >  1:615431/2‑149 (MQ=255)
                                                                                                                                               cgAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGCAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCt  >  1:261128/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCT  >  NC_000913/4186457‑4186750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: