Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,413,7610CT41.5% 47.0 / 24.3 41G56E (GGA→GAA) ralRRac prophage; restriction alleviation protein
Reads supporting (aligned to +/- strand):  ref base C (6/18);  new base T (17/0);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 6.89e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGG  >  NC_000913/1413616‑1413906
                                                                                                                                                  |                                                                                                                                                 
ccTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACACCTCCATTATTATTTCCAGTg                                                                                                                                              <  1:300081/151‑1 (MQ=255)
  tGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTATATTAAAACCCCCCTTTTTTTTTTCacttg                                                                                                                                             >  1:465727/1‑146 (MQ=255)
   gCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACACCTCCATTATTATTTCCAGTgg                                                                                                                                             <  2:679232/149‑1 (MQ=255)
      tcttTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCg                                                                                                                                         <  1:55534/150‑1 (MQ=255)
            ttACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AAATTTTTAAATGAAAAACCCCCTTATTATTTTCAGTGGTTCGtttttt                                                                                                                                   >  1:186158/1‑150 (MQ=255)
             tACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTACATGAAAACCCCCCTTTTTTTTTTCCGTGGGTTGtttttt                                                                                                                                   >  1:227098/1‑149 (MQ=255)
              aCCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCa                                                                                                                                <  2:23260/151‑1 (MQ=255)
                 tGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGA‑AATTTTTTTCATGAAAAACCACTTTATTTTTTTCAGTGGTTCGGTTTTTTCAtt                                                                                                                              >  2:359883/1‑149 (MQ=255)
                          tttttCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTg                                                                                                                     <  1:502448/150‑1 (MQ=255)
                             ttCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑TATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGctt                                                                                                                  <  2:207261/150‑1 (MQ=255)
                             ttCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑CATTTTTTTCATAAAAAACCCCCTTATTATTTTCGATGGGTCGTTTTTTTCATCCTTcctaggcttt                                                                                                                 >  1:459147/1‑142 (MQ=255)
                              tCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AAATTTTTACATTAACACCCCCCTTTTTTTTTTCACTGGGTTGGTTTTTTCATTTTTCTAGTGgttct                                                                                                                >  1:542718/1‑151 (MQ=255)
                                gCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCttt                                                                                                              <  2:213362/151‑1 (MQ=255)
                                 cATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCtttt                                                                                                             <  1:136237/151‑1 (MQ=255)
                                   ttCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAAATTTTTTAACTAAAAACCCCCTTTATTTTTTCC‑GGGGTTTGTTTTTTTCATCTTTTTAGTGGttttttttt                                                                                                           >  1:236572/1‑150 (MQ=255)
                                    tCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTAAATGAACACCCCCCTTTTTATTTTCCGTGGTTCGTTTTTTTCATCTTTTGAGTGCTTCttttt                                                                                                            >  1:216228/1‑149 (MQ=255)
                                     cTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTATATTAACACCTCCCTTTTTTTTTTTTGTGGGTTGTTTTTTTTATTTTTTTAGTGGtttttttttt                                                                                                          >  2:436203/1‑148 (MQ=255)
                                        aTTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACtt                                                                                                       <  2:69591/150‑1 (MQ=255)
                                        aTTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGG‑AATTTTTTACATTAACACCTTCCTTTTTTTTTTTTGTGGGGGGTTTTTTTTTTCTTTCTATTGCtttttttt                                                                                                            >  2:360128/1‑145 (MQ=255)
                                         tttCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATTTATAAACACCACCCTTTTTATTTTCACCGGTTTGCTTTTTTTATCTTTTCAGTGTTTCTTTTTTACTTc                                                                                                      >  1:154279/1‑150 (MQ=255)
                                          ttCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTcc                                                                                                     <  1:314659/150‑1 (MQ=255)
                                           tCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTcca                                                                                                    <  2:88423/150‑1 (MQ=255)
                                               tAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTACATGAACACCCCCCTTATTTTTTTCCGTGGGTTGTTTTTTTCCTCTTTTTAGTGGTTTTTTTTTTCTTtcacc                                                                                                  >  1:462076/1‑148 (MQ=255)
                                                       aTTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTc                                                                                       <  1:124336/151‑1 (MQ=255)
                                                         tttGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AAATTTTTAAATTAACACCCCCATTTTTTTTTTCCGTGGGTTGTTTTTTTCCTTTTTCCAGTGCTTCTTTTTTTCTTTCACCCTAAcccggt                                                                                        >  1:485141/1‑144 (MQ=255)
                                                                 tCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTTAATTAAAAACCCCCTTTTTTTTTTCCGTGGTTTGTTTTTTTCCTCTTTTTGGTGCTTTTTTTTTACTTCCCCCCTAAACCGGTCGGGGtt                                                                                 >  1:105623/1‑147 (MQ=255)
                                                                 tCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTATATGAAAACCTCCCTTTTTTTTTCCAGGGGGTTGGTTTTTTTATCTTTTCCGTGGTTTTTTTTTTTTTCCCCCCTAACCCGTTCTGGGtt                                                                                 >  2:392904/1‑147 (MQ=255)
                                                                    tGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGC‑AATTTTTTTTAAGAACACCTCCCTTTTTTTTTTTCGTGGGTCGTTTTTTTCATTTTTCTAGGGGTTTTTTTTTTCTTTCACCCTAAACCGTTCCGGGTTTGCATc                                                                           >  2:703693/1‑150 (MQ=255)
                                                                            tagtagGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTAAATGGAAACCACCCTTATTATTTTCTGTGAGTTGTTTTTTTCATTTTTTGCTGGGTTCTTTTTTACTTCCCCCATAAACCGGTCGGGGTTTGCATTCGTTaaa                                                                    >  1:637075/1‑147 (MQ=255)
                                                                             agtagGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACt                                                                  <  2:373071/150‑1 (MQ=255)
                                                                                            ttccttTTCAATAGTGGCGGT‑AAATTTTTACATTAAAACCTCCCTTATTATTTTCAGTGGTTATTTTTTTTCATTTTTCTCGTTCTTCTTTTTTACTTTCCCCCTAACCAGTTTGGGTTTTGCATCCCTTTCACTTTTCCCTGTAata                                                     >  2:339292/1‑148 (MQ=255)
                                                                                              ccttTTCAATAGTGGCGGT‑AATTTTTTTCATTAAAACCCCCCTTTTTTTTTTTTTTGGTTTGTTTTTTTCATCTTTTCCGTGGTTTTTTTTTTCTTTCCCCATAAACGGGTCGGGGGTTGCCTCCCTTATACTTCCCTCCGGAatattc                                                  >  1:661556/1‑147 (MQ=255)
                                                                                                   tCAATAGTGGCGGT‑AATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAAt                                            <  1:548774/150‑1 (MQ=255)
                                                                                                        aGTGGCGGT‑AATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCtt                                       <  1:430768/150‑1 (MQ=255)
                                                                                                           ggCGGT‑AATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCa                                    <  2:223662/150‑1 (MQ=255)
                                                                                                                     tttATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTg                           >  2:545768/1‑150 (MQ=255)
                                                                                                                           cATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGAc                     >  1:693/1‑150 (MQ=255)
                                                                                                                            aTGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACa                    >  2:117397/1‑150 (MQ=255)
                                                                                                                               aaCACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACca                <  1:359928/151‑1 (MQ=255)
                                                                                                                                  acCTCCATTATTATTTCCAGT‑GTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACaaa            <  2:190169/151‑1 (MQ=255)
                                                                                                                                             tattTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATgg  >  1:177165/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGG  >  NC_000913/1413616‑1413906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑