Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,522,182	C→A	T190T (ACG→ACT)	hofM ←	DNA catabolic putative pilus assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,522,182	0	C	A	100.0%	164.4 / NA	46	T190T (ACG→ACT)	hofM	DNA catabolic putative pilus assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/27);  total (19/27)

GACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGAACACGCAACCTTTCTGCCAGAGTAAGCAGCGTT  >  NC_000913/3522043‑3522329                                                                                                                                            |                                                                                                                                                    gACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCcac                                                                                                                                            >  2:369319/1‑149 (MQ=255) gACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGACCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAg                                                                                                                                          <  2:527509/151‑1 (MQ=255)    gAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGcc                                                                                                                                        >  1:594843/1‑150 (MQ=255)     aaCAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCAc                                                                                                                                      <  1:142899/151‑1 (MQ=255)            aCGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGt                                                                                                                               >  1:339618/1‑151 (MQ=255)               gCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTAt                                                                                                                            >  1:389869/1‑151 (MQ=255)                 cTCCCAGGGATCAAATCCGCCTTCGCCACCTATCGCGACGCTTTCGGGATCAACGCATAACGCTGCCGCCAGCTCCTCCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATACCGAGTCGCCCACAGCCACTGTTCGTTATc                                                                                                                           >  2:589519/1‑150 (MQ=255)                   cccAGGGATAAAACCCGCCTTCGCCCCATATCGCGCCGCTTCCGGGATCACCGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACg                                                                                                                        <  2:293229/151‑1 (MQ=255)                    ccAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACgc                                                                                                                       >  1:550351/1‑151 (MQ=255)                       gggATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACgccag                                                                                                                    <  1:483364/151‑1 (MQ=255)                            cAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACgccaggcc                                                                                                                 >  1:417423/1‑149 (MQ=255)                              aaTCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGAGAACGCTGCCGCCAGCTCCTTCGCGCTAGTCACCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGAc                                                                                                             <  2:595610/151‑1 (MQ=255)                              aaTCCGCCTTCGCCACATATCGCGACGCTTGCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACgccaggcca                                                                                                                <  2:204504/148‑1 (MQ=255)                               aTCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGAc                                                                                                             >  1:581433/1‑150 (MQ=255)                               aTCCGCCTCCGCCACATATCGCGCCGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGa                                                                                                              <  2:276367/149‑1 (MQ=255)                                       tCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCtg                                                                                                      <  1:243025/149‑1 (MQ=255)                                          ccACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATgag                                                                                                 <  2:126001/151‑1 (MQ=255)                                                atCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGt                                                                                            <  1:227544/150‑1 (MQ=255)                                                  cgcgACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCGCAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTaaa                                                                                         <  1:637711/151‑1 (MQ=255)                                                  cgcgACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTa                                                                                           >  2:276848/1‑149 (MQ=255)                                                   gcgACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTaaa                                                                                         >  1:301682/1‑150 (MQ=255)                                                     gACGCTTTCGGGATCACCGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTaaaaaa                                                                                      <  2:418040/151‑1 (MQ=255)                                                         cTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGc                                                                                   >  1:644207/1‑150 (MQ=255)                                                           ttCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAgg                                                                                >  1:374896/1‑151 (MQ=255)                                                            tCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAgg                                                                                >  1:601175/1‑150 (MQ=255)                                                             cGGGATCAACGGATAGCTCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCAGTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAg                                                                                 <  2:154409/148‑1 (MQ=255)                                                                  tCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCt                                                                         >  2:365157/1‑151 (MQ=255)                                                                    aaCGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGt                                                                       >  1:74201/1‑151 (MQ=255)                                                                     aCGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGt                                                                       >  2:264297/1‑150 (MQ=255)                                                                      cGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTa                                                                      <  1:395694/150‑1 (MQ=255)                                                                         aTAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGc                                                                  <  2:445813/151‑1 (MQ=255)                                                                            aCGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACt                                                               <  1:511034/151‑1 (MQ=255)                                                                                 gccgccAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGc                                                            <  1:549587/149‑1 (MQ=255)                                                                                 gccgccAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCa                                                           <  1:178383/150‑1 (MQ=255)                                                                                  ccgccgGCTCCTTCGCGCTAGTCATCCCCGCCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCAACAGCCACTGTTCGTTATCGCGCCAGGCCGGACATTGGTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATgaa                                                                 <  2:48953/143‑3 (MQ=255)                                                                                  ccgccAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATc                                                         <  1:711763/151‑1 (MQ=255)                                                                                        gCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCgggg                                                    <  2:509961/150‑1 (MQ=255)                                                                                           ccTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGa                                                 >  2:158612/1‑150 (MQ=255)                                                                                                gcgcTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCgcgc                                           >  2:438866/1‑151 (MQ=255)                                                                                                   cTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTc                                         >  2:512734/1‑150 (MQ=255)                                                                                                    tggTCATCCCCCCCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCGACAGCCACTGTTCGTTATCGCGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTc                                         <  2:606581/147‑1 (MQ=255)                                                                                                              cACCGCCAGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGGGATCGCGCTCACATGAACACGc                             <  2:246649/151‑1 (MQ=255)                                                                                                                     aGTTTGCGCCCCCAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGAACACGCAACCttt                      <  1:369448/151‑1 (MQ=255)                                                                                                                       tttGCGCCCCCAGATATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACAGTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGAACACGCAAccg                        <  2:64018/147‑2 (MQ=255)                                                                                                                             cccccAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGAACACGCAACCTTTCTGCCaga              <  2:451763/151‑1 (MQ=255)                                                                                                                                 cAGCTATAGCGAGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGAACACGCAACCTTTCTGCCAGAGTaa          <  2:22032/151‑1 (MQ=255)                                                                                                                                         gcgaGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGAACACGCAACCTTTCTGCCAGAGTAAGCAGCGtt  <  1:659374/151‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                    GACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGAACACGCAACCTTTCTGCCAGAGTAAGCAGCGTT  >  NC_000913/3522043‑3522329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑