Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,725,643	Δ1 bp	coding (104/1299 nt)	kgtP ←	alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,725,642	0	C	.	100.0%	177.9 / NA	37	coding (105/1299 nt)	kgtP	alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (21/16);  total (21/16)

CAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTC  >  NC_000913/2725505‑2725787                                                                                                                                          |                                                                                                                                                  cAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGAtt                                                                                                                                     >  1:85600/1‑151 (MQ=255)    ccGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTAcc                                                                                                                                  >  2:230130/1‑151 (MQ=255)         tATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGa                                                                                                                               >  2:378980/1‑149 (MQ=255)            tGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAgg                                                                                                                          >  1:402958/1‑151 (MQ=255)             gggCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGc                                                                                                                         >  1:24593/1‑151 (MQ=255)                            cccGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTTCCTGAAGAGGCCCCCACAATCGccc                                                                                                           <  2:250495/150‑1 (MQ=255)                             ccGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCa                                                                                                          >  2:118745/1‑150 (MQ=255)                               gcagcaAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAAt                                                                                                       >  1:682957/1‑151 (MQ=255)                                 agcaAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATgc                                                                                                     >  2:483424/1‑151 (MQ=255)                                 agcaAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATgc                                                                                                     >  2:530011/1‑151 (MQ=255)                                   caAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATgcgg                                                                                                   <  2:51898/151‑1 (MQ=255)                                           ccTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGt                                                                                           >  1:8439/1‑151 (MQ=255)                                                      tagtagTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTg                                                                                >  1:535083/1‑151 (MQ=255)                                                       agtagtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACGCGGCCATATGACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACttt                                                                                <  2:537154/150‑2 (MQ=255)                                                          agtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCa                                                                             <  2:232258/150‑1 (MQ=255)                                                             tGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGtt                                                                          <  2:593585/150‑1 (MQ=255)                                                               aGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGc                                                                       >  2:298853/1‑151 (MQ=255)                                                                   gtcgtGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTc                                                                   <  1:40133/151‑1 (MQ=255)                                                                     cgtGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCt                                                                  <  2:267505/150‑1 (MQ=255)                                                                      gtgtTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGc                                                                >  2:133980/1‑151 (MQ=255)                                                                         ttCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGt                                                             >  1:578041/1‑151 (MQ=255)                                                                          tCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGtt                                                            >  1:556225/1‑151 (MQ=255)                                                                             cGGAAGGGAAGATGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTAc                                                          >  1:65566/1‑150 (MQ=255)                                                                                 agggaagAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTAc                                                     >  2:505333/1‑151 (MQ=255)                                                                                      agaagaTGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCa                                                <  2:340452/151‑1 (MQ=255)                                                                                      agaagaTGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCa                                                <  2:470193/151‑1 (MQ=255)                                                                                       gaagaTGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCC‑AATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgc                                              >  1:557351/1‑151 (MQ=255)                                                                                           aTGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgccat                                           <  2:243536/151‑1 (MQ=255)                                                                                           aTGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgccat                                           <  1:381892/151‑1 (MQ=255)                                                                                               gggCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgccatgcc                                        <  1:530213/150‑1 (MQ=255)                                                                                                          agTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCt                            <  2:264521/151‑1 (MQ=255)                                                                                                           gTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTg                           >  2:599321/1‑151 (MQ=255)                                                                                                              aaCAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCg                        <  1:148958/151‑1 (MQ=255)                                                                                                                           gACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCtt           >  2:445730/1‑151 (MQ=255)                                                                                                                               tAGAAATCGAA‑CACTCGCCCAGATTACCTGAAGAGGCCCCCCCAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCtttt         <  1:230189/149‑1 (MQ=255)                                                                                                                                aGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTgtc      >  1:255901/1‑151 (MQ=255)                                                                                                                                    aTCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTgtcggtc  <  1:334813/151‑1 (MQ=255)                                                                                                                                          |                                                                                                                                                  CAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTC  >  NC_000913/2725505‑2725787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑