| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,880 | 0 | T | C | 57.1% | 7.9 / 32.9 | 28 | K271E (AAA→GAA) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base T (8/4); new base C (0/16); total (8/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.59e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.51e-05 | |||||||||||
TAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCG‑TGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGC‑GGCGGCTGTCTGCCAG‑CTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGT > NC_000913/357735‑358026 | tAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCG‑TGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGC‑GGCGGCTGTCTGCCAG‑CTATTTTTccgcc > 2:488419/1‑151 (MQ=255) aGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCG‑TGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGC‑GGCGGCTGTCTGCCAG‑CTATTTTTccgccg < 2:640485/151‑1 (MQ=255) ggCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCG‑TGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGC‑GGCGGCTGTCTGCCAG‑CTATTTTTccgccg < 2:588893/150‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCG‑TGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGC‑GGCGGCTGTCTGCCAG‑CTATTTTTCCGCCGCAACaaa < 1:226592/151‑1 (MQ=255) gcATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCG‑TGATTCATTGCCGCCAACAACCGCGCATTCGTCCAACGCCATGTGCAAAAATGCCTTCGC‑AGCGGCTGTCTGCCAG‑CTATAGTTGCGCCGgaagaggcgcgcgaccc > 2:201886/1‑136 (MQ=14) aGCCGCAGTCTTGTGTAGGCGG‑TACCGTTTATTCATTCCCCCCCCAAACCGCGCTTTTCCCAACCGCTTTTTAAAAAAATCCCTTCGC‑GGCGGCTGTCCCCAAT‑TTTTTTTCCCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCg < 1:70507/150‑1 (MQ=255) ccagtcctcgctcggCGGCTAGCG‑TGATCCATTCCCCCCACCACCGGCGCTTTTACCAACCGCCTGGTGCAAAAAGGCGTTCGC‑GGCGTCTTTCGGCCGG‑CTTTTTTCCCCCCGAAAGAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCa < 2:590597/137‑1 (MQ=255) cttgtgTCAGGGCCTACCG‑TGATTCTTTCCCGCCAACAACCGCGCATTTACCCAGCGCCAGGTGAAAAAATGCCTTCGC‑GGCGGCTGTCTGCCAG‑TTATTTTCCCGCCGCAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAagag < 1:248212/148‑1 (MQ=21) ccTGTGTCAGCGGCTACCG‑TGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGC‑GGCGGCTGTCTGCCAG‑CTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggggga > 1:474448/1‑144 (MQ=255) gtgtCAGCGGCTACCG‑TGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGC‑GGCGGCTGTCTGCCAG‑CTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGGGAGaaa > 2:218934/1‑150 (MQ=255) tgtCGGCGGCTCCCG‑TGATTCATTCCCCCCACCACCCGCGCTTTTCCCCACCGCCTTGTGAAAAAATGCTTTCGG‑GGGGGCTGTCTGCCAG‑CTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAAt < 1:219062/150‑1 (MQ=255) gtCAGCGGCTACCG‑TGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGC‑GGCGGCTGTCTGCCAG‑CTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGAGaaaa > 1:323489/1‑148 (MQ=255) ccgcggccccccG‑TAATTCATTCCCCCCACCACCCCCCCTTTTACCCACCCCCAGGTCAAAAAATCCCTCGGG‑GGCGGCGGTCTGCAAG‑TTTTTTTCCCGCCCAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAagag < 2:686003/134‑1 (MQ=255) cgtctcccG‑TGTTTCTTTCCCCCCAACACCCGCGTGTTTACCCACCCCCTTGTAAAAAAATCCCGCCGC‑GGGGGCTTTCCGCCTG‑TTTTTTTCCCGCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAaga < 2:92383/133‑1 (MQ=255) ccttgtttCATTTCCCCCAACACCCGCCCTTTTACCCACCGCCATGGGAAAAAATCCCTTCGC‑GGCGGCT‑TCCTCCAGTCGATTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGccc < 2:620218/144‑1 (MQ=255) ccgtttttCTTTCCCGCAACCACCCGCCCATTTCTCCACCGCTGTGAGAAAAAAT‑CCCTCGCGGGGGGTTGTCTGCAAT‑CTTTTTTCCCGCCGAAAAAAATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCtt < 2:678637/137‑1 (MQ=255) cccGCCCACACCCGCTCTTTTCCCCACCTCCATGTCCAAAATGGCCTTCGC‑TGCGGCTGCCCGCCGT‑CTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCAtgttgt < 1:201934/147‑1 (MQ=255) cccccAACACCCGCGCTTTTACCCAACGCCTTGTGAAAAAATGCTTTCGC‑GGCGGTTGTCTCCCAG‑CTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTgg < 1:206357/148‑1 (MQ=255) ccGCCAACACCCGCGCTTTTACCCAACGCCTTGTGAAAAAATGCCTTCGC‑GGCGGCTGTCTGCCAG‑TTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTgg < 1:77219/151‑1 (MQ=255) cGCCACCACCCGCGCTTTCACCCACCCCCGTGGGAAAAAATGCCGCCGC‑GGCGGCTGCCTGCCAT‑TTATTTTTCCGCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGc < 2:81075/151‑1 (MQ=255) acccACCGCCATTTGAAAAAATCCCTTCGG‑GGCGGTTGTCTGCAAG‑CTTTTTTCCCGCCAAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACa < 2:283478/147‑1 (MQ=255) accccaaTGTGAAAAAATCCCTTCGC‑GGCGCCTGTCGCCAAG‑CTATTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTgg < 1:157019/145‑1 (MQ=255) cATGTGAAAAAATGCCTCCGC‑GGCGGCTGTCTGCCAG‑CTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAgg < 1:73266/151‑1 (MQ=255) ggcggtTGTCGGCCAG‑TTTTTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCa < 1:191949/146‑1 (MQ=255) gcggcTGTGTCCGAG‑TTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCa < 2:14563/145‑1 (MQ=255) tGTCTGCCAG‑CTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAGAAAAAACCTTTAACCCCCCCTGTTTTGTGGGGATCGCCGCTGGTGACAAATTGGGAAAGGGAGGGCGGCGGAACAACTcccaaa > 1:516302/1‑145 (MQ=255) tGTCTGCCAG‑CTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGAGAGAAAAAACTTTTAACCCCCCCTTTTGTGTGGGGATCGCTGCTGGTGACAAAATGGGAAGGGGAGGGGGGGGGATCAACTccccaa > 1:207012/1‑145 (MQ=255) ccAG‑CTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGCGAGAAAAAAAATTAAACCCCCCCCTTTGTGGGGCGCTCGCTGCTGGGAAAAATGTGGGAAGGGAAGGGCGGGGGAGCAGCTCCCGaaaacc > 2:40509/1‑143 (MQ=255) ttttttcCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAAtt < 1:72917/147‑1 (MQ=255) tttcCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGt < 1:542088/150‑1 (MQ=255) | TAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCG‑TGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGC‑GGCGGCTGTCTGCCAG‑CTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGT > NC_000913/357735‑358026 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |