Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,483,254	0	G	A	43.6%	40.2 / 20.4	39	S29L (TCA→TTA)	emrK	multidrug resistance efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base G (13/9);  new base A (0/17);  total (13/26)
Fisher's exact test for biased strand distribution p-value = 7.83e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATT  >  NC_000913/2483104‑2483397                                                                                                                                                       |                                                                                                                                                ccTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTg                                                                                                                                                 >  1:202389/1‑151 (MQ=255)     gTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGaaa                                                                                                                                              <  1:56185/150‑1 (MQ=255)         aaCGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGc                                                                                                                                          >  2:351382/1‑150 (MQ=255)           cGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaat                                                                                                                                       <  2:443390/151‑1 (MQ=255)            gTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCTCGTGACATAGGCGTCATATGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaa                                                                                                                                        <  2:161434/149‑1 (MQ=255)             tAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaata                                                                                                                                      >  2:618765/1‑150 (MQ=255)               gTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaat                                                                                                                                   >  2:651930/1‑151 (MQ=255)               gTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaat                                                                                                                                   >  2:340307/1‑151 (MQ=255)                           aTGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGcc                                                                                                                       >  1:145356/1‑151 (MQ=255)                                 aaCGACAGTGACACTACCTGAGACTTGTGCAGCAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATaa                                                                                                                  >  2:428981/1‑150 (MQ=255)                                    gACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGa                                                                                                               >  2:466623/1‑150 (MQ=255)                                        gTGACACTACCTGAGATTTGTGCAGAAATGGGATCGGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATTTCTTTTAATTCCATTGACCAATGGGAATAGGCACCAAAAACCGAAATAAATAAACCTACCGCCAATAAAGAAAAg                                                                                                           <  1:471963/150‑1 (MQ=255)                                           acacTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTAtt                                                                                                       >  1:529802/1‑151 (MQ=255)                                                 ccTGAGACTTGTGCAAAAATTGGTTCTGCATCCCCCGTGACATAGGCCTCATCTGTACTAATCATGTCTACTAATTCCATTGACCAAAAGGAAAAGACACCTAAAACCGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTttctt                                                                                                  <  2:217398/150‑1 (MQ=255)                                                     ataCTTGTGCAAAAATTGGATCTGCATTCCCCGTGAAATAGGCGTCATCTGTACTAATCATGTCTTTAAATTCAATTGCCCAATAGGCATAGGCACCTAAAACCGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGtt                                                                                             <  1:406114/149‑1 (MQ=255)                                                         ttttgaaGAAATTGTATCTGCTTCCCCCGTAACATAGGCTTCATCTGTACTAATCATGTCTTATAATTCAATTGACCAATAGAAATAGCCACCTAAAAACGAAAAAAATAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGa                                                                                          <  2:183849/144‑1 (MQ=255)                                                         ttGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAg                                                                                           <  1:428284/149‑1 (MQ=255)                                                               aaaaaTTGTATCTGTATTCCCCGTGAAATGGGCGTCATCTGTACAAATCATGTTTTCTAATCCCATTGCCAAAAAGGAATGGACCCTTAAAACCGAAAAAAAAAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATg                                                                                       <  2:441723/145‑1 (MQ=255)                                                                 aaaTTGGATCTGCATCCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTAAAAACGAAATAAAAAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtttttt                                                                                 <  1:13568/151‑1 (MQ=255)                                                                    ttGGATCTGCATTCCCCGTGACATAGGCTTCATCTGTACTAATCATGTTTTCTAATCCCATTGACAAATAGGCATAGGCACCTGAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAt                                                                               <  1:481350/150‑1 (MQ=255)                                                                      ggATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACAAATAGGCATAGGCACCTGAAAACGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAttt                                                                             <  1:539821/150‑1 (MQ=255)                                                                         tctcCATTCCCCGTGAGATAGGCGTCATCGGTACTAACCATGTCTTTTAATCCCATCACCAAATGGGCATAGGCACTTAAAACCGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGa                                                                           <  1:206161/149‑1 (MQ=255)                                                                            gCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTa                                                                       >  2:88927/1‑150 (MQ=255)                                                                              aTTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATc                                                                    >  1:123330/1‑151 (MQ=255)                                                                               ttCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATGGACCAATAGGCATAGGCACCTAAAAACGAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGaa                                                                          <  1:87616/144‑1 (MQ=255)                                                                                 ccccGTGACATAGGCGTTATCTGTAATAATAATGTCTTCTAATCCCATTAACCAATAGGCATAGAACCAAAAAAACGAAATAAAAAAACTTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGt                                                                 <  1:79777/151‑1 (MQ=255)                                                                                  cccGTGACATAGGCGTCATTTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGtt                                                                <  1:114665/151‑1 (MQ=255)                                                                                       gACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACt                                                           >  2:502426/1‑151 (MQ=255)                                                                                        aCATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTAAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACt                                                           <  1:481734/150‑1 (MQ=255)                                                                                                gTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtctct                                                  >  1:262412/1‑151 (MQ=255)                                                                                                      tGTACTAATCATTTCTTCTAATTCCATTGACCAATAGGCATAGGCCCCTGAAAACTCAATAAAAAAAACTTCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTATTCTGTTCCACTATTAtctctc                                                 <  2:641417/146‑1 (MQ=255)                                                                                                            aaTCATGTCTTCTAATCCCATTGACCAATAGGCATAGGCACCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtc                                            <  1:652161/145‑1 (MQ=255)                                                                                                            aaTCATGTCTTCAAATTCCATTGACCAATAGGCATAGGCACCTAAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtct                                           <  1:574890/146‑1 (MQ=255)                                                                                                                         aaTTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACTGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAt                           <  1:197001/149‑1 (MQ=255)                                                                                                                         aaTTCCATTGACCAATAGAAAAAGGAACCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAAtt                              <  2:351712/146‑1 (MQ=255)                                                                                                                          aTTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtga                         >  2:18668/1‑150 (MQ=255)                                                                                                                           ttCCATTGACCAATAGGCATAGGCACCTAAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaa                        <  1:549781/150‑1 (MQ=255)                                                                                                                             caaTTAACCAAAAGGAAAAGCCACTTAAAAACTAAAAAAAAAAAATTTCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaatt                      <  2:589044/148‑1 (MQ=255)                                                                                                                                         aggcataggcaCCTAAAAACGAAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAagag          <  2:68948/150‑1 (MQ=255)                                                                                                                                                gccaCCGGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattcatt  <  1:181836/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                CCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATT  >  NC_000913/2483104‑2483397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑