Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,6530TG42.4% 35.0 / 11.9 33P46P (CCA→CCC) dppBdipeptide/heme ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (13/6);  new base G (0/14);  total (13/20)
Fisher's exact test for biased strand distribution p-value = 5.04e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

CACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGATT  >  NC_000913/3705512‑3705800
                                                                                                                                             |                                                                                                                                                   
cACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATc                                                                                                                                            <  2:244908/151‑1 (MQ=255)
          tCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGtt                                                                                                                                     <  2:94983/148‑1 (MQ=255)
          tCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTc                                                                                                                                    <  2:17078/149‑1 (MQ=255)
             cAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTcgcccg                                                                                                                               <  2:179999/151‑1 (MQ=255)
                aCCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTcgcccgcc                                                                                                                             >  1:209379/1‑150 (MQ=255)
                           gCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCAcc                                                                                                                 >  2:430279/1‑151 (MQ=255)
                             ttttCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCAGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCAAGTTCGCCCGCCATGATCATCACCg                                                                                                                >  2:558284/1‑150 (MQ=255)
                                 cATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCg                                                                                                           >  1:653577/1‑151 (MQ=255)
                                   ttaaaaTGCCTAGACCGCCATGCAAACCCCCCAAAATGTAATGGAGATCCTGCTCCCACATCGTTTTATCAAAGCCGAGTTCAGCCAGCGGCTGCGCGTGCCGCTCGGGGGAGACCCCACGTTCGCCCGCCATGATCATCACCGGATCg                                                                                                           <  1:185547/146‑1 (MQ=255)
                                      aaaTGCCTAGCCCGCCATGAATACCCCCCAAAAGGAAAGGGAGATCCTGCCGCCACGTCGTTTTATCAAAGCCGAGTCCGCCCAGCGGCGGGGGGGGCCGCGGGGGGGAATCCCCACTTCCCCCCGCCATGATCATCACCGGATCg                                                                                                           <  2:438918/146‑1 (MQ=255)
                                      aaaTGCCTAGATCGCCATGCAAACCGCCCAAAATGTAATGGAGATACTGCTGCCACATGGTTTTATCAAAGCCGAGTTCAGCCAGCCGCTGCGCGTGACGCTCGGGGGAGACCCCACGTCCGCCCGCCATGATCATCACCGGATCGCCCg                                                                                                       <  1:136707/150‑1 (MQ=255)
                                      aaaTCCCTAGATCCCCATGCATACCCCCCAAAATGAAAGGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGCCGCTCGGGGGAGACCCCACGTTCGCCCGCCATGATCATCACCGGATCGcc                                                                                                         <  1:422382/148‑1 (MQ=255)
                                      aaaTCCCTAGACCCCCATGCATACCCCCCAAAATGAAAGGGAGATCCTGCTCCCACATGGTTTTATCTAAGCCGATTCCACCCAGCAGCGGGGCGTGCCGCTCGGGGGAAACCCCCCGTCCGCCCGCCATGATCATCACCGGATCGccc                                                                                                        <  1:294305/149‑1 (MQ=255)
                                           ccTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATc                                                                                                  >  2:180187/1‑150 (MQ=255)
                                           ccTAGACCCCCATGCATACCCCCCAAAATGAAATGGAGATACTGCTGCCACATCGTTTTATCTAGGCCGAGTCCAGCCAGCAGCGGCGCGGGCCGCTCGGGGGAGACCCCACGTCCGCCCGCCATGATCATCACCGGATCGCCCGGGATc                                                                                                  <  1:443755/150‑1 (MQ=255)
                                                 ccGCCATCCAAACCCCCCAAAAGGTAAGGGGGATCCTCCGGCCACATCGTTTTACTAAAGCCGATTCCAGCCGGCAGCTGCGGGGGACGGGCGGGGGAGACCCCGCGTCGCCCCGCCATGATCATCACCGGATCGCCCGGGATCAtgt                                                                                              <  2:651951/147‑1 (MQ=255)
                                                   gCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCCCTGGGGAGATCCCCCGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGCGGAc                                                                                          >  2:481806/1‑150 (MQ=255)
                                                    ccATGCAAACCGCCCAAAAGGTAAGGGGGGTGCGGCGGCGACGGGGGTTTACCTACGCCGAGTTCAGCACCCCGCTCCGGGTGAGCCTCGGGGGAGACCCCATGTCCGCCCGCCATGATCATCACCGGATCGCCCGGGATCAtgt                                                                                              <  2:116390/145‑1 (MQ=255)
                                                            aaCGCCCCAATTGTAGTGGAGATCCCGCCCCCACTTCGTTTTATCTAACCGGAGTTCAGCCCGGCGCGGCGCGCGGCGCGGTGGGGAGATCCCCCCTCCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCaaa                                                                                 <  1:319917/150‑1 (MQ=255)
                                                                             ggAAATACTCCCCCCACATCGTTTTATCAAAGCCGAGTCCCGCCAGCAGCTGCGCGTGACGCTGGGGGGAGACCCCACGTTCCCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATAcc                                                               <  1:626411/151‑1 (MQ=255)
                                                                              gagaTACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCg                                                              >  2:224842/1‑151 (MQ=255)
                                                                              gagaTACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCg                                                              >  2:11934/1‑151 (MQ=255)
                                                                                       ctgcCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTg                                                     >  1:348330/1‑151 (MQ=255)
                                                                                                   tttATCAAGGCGGATTTCAGCCAGCGGCGGCGCGGGCCGCTGGGGGGAGCCCCCCCGTGCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACg                                                       <  1:622580/137‑1 (MQ=255)
                                                                                                    ttATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTc                                        >  1:671113/1‑151 (MQ=255)
                                                                                                              cGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCg                              >  1:135840/1‑151 (MQ=255)
                                                                                                                     agccagcAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAAt                        >  1:671381/1‑150 (MQ=255)
                                                                                                                           cggcggcggGTGGCGCTGGGGGGAGACCCCCCGTCCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAAt                        <  1:46186/139‑1 (MQ=255)
                                                                                                                             gttccgcgGGACGCTGTGGGGGGAGCCCCCGTCCCGCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGc                 <  2:137775/145‑1 (MQ=255)
                                                                                                                                gcgcGTGCCGCTCGGGGGAGACCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAAc              <  1:453284/149‑1 (MQ=255)
                                                                                                                                    gTGCCGCTGGGGGAAGACCCCACGTCCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCa                <  1:598154/143‑1 (MQ=255)
                                                                                                                                      gccGCTCGGGGGAGACCCCCCGTCCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAAccc       <  1:287430/148‑1 (MQ=255)
                                                                                                                                         gCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGAt   >  1:445940/1‑151 (MQ=255)
                                                                                                                                          ctctGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGAtt  >  1:303420/1‑151 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
CACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGATT  >  NC_000913/3705512‑3705800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑