Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	481,075	A→G	intergenic (‑367/+179)	tomB ← / ← acrB	Hha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	481,075	0	A	G	100.0%	180.0 / NA	45	intergenic (‑367/+179)	tomB/acrB	Hha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/22);  total (23/22)

ACTATTCATAACATTTCAGGATTATCGCCAGATGAAATTGGTGACACTAATACCAGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCC  >  NC_000913/480929‑481222                                                                                                                                                   |                                                                                                                                                    aCTATTCATAACATTTCAGGATTATCGCCAGATGAAATTGGTGACACTAATACCAGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATc                                                                                                                                                 >  2:450976/1‑151 (MQ=255) aCTATTCATAACATTTCAGGATTATCGCCAGATGAAATTGGTGACACTAATACCAGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATc                                                                                                                                                 <  1:496079/151‑1 (MQ=255)  cgaTTCATAACATGTCAGGATTATCGCCAGATGAAATTGGTGACGGTAATACCAGGATGGCTCTGAATATGACGTAAGAACCGAGGAATGAATAAAGAATTACCGCAAATAAGTAGGAATAGCGTCTAAATGGTTATGGGTTAGTGAAtg                                                                                                                                                 <  2:317960/148‑2 (MQ=255)    aTTCATAACATTTCAGGATTATCGCCAGATGAAATTGGTGACACTAATACCAGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTAt                                                                                                                                              >  1:4780/1‑151 (MQ=255)       cATAACATTTCAGGATTATCGCCAGATGAAATTGGTGACACTAATACCAGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAg                                                                                                                                           <  1:521232/151‑1 (MQ=255)             aTTTCAGGATTATCGCCAGATGAAATTGGTGACACTAATACCAGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTAc                                                                                                                                     <  1:28198/151‑1 (MQ=255)                     aTTATCGCCAGATGAAATTGGTGACACTAATACCAGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAAtata                                                                                                                             <  2:661337/151‑1 (MQ=255)                        aTCGCCAGATGAAATTGGTGACACTAATACCAGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAAt                                                                                                                          <  1:486206/151‑1 (MQ=255)                            ccAGATGAAATTGGTGACACTAATACCAGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATtaac                                                                                                                      <  1:390159/151‑1 (MQ=255)                                aTGAAATTGGTGACACTAATACCAGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATtaactaac                                                                                                                  >  2:405269/1‑151 (MQ=255)                                     aTTGGTGACACTAATACCAGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCa                                                                                                             <  1:4266/151‑1 (MQ=255)                                         gTGACACTAATACCAGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCGACTTAATATAAATTAACTAACAATCAGTAt                                                                                                         <  2:550085/151‑1 (MQ=255)                                              acTAATACCAGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTAtttttt                                                                                                    <  1:152240/151‑1 (MQ=255)                                                     ccAGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAAtt                                                                                             >  1:297892/1‑151 (MQ=255)                                                            tGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCg                                                                                      >  1:615048/1‑151 (MQ=255)                                                               tctGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTgg                                                                                   >  1:217248/1‑151 (MQ=255)                                                                  gAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTgg                                                                                   <  2:45508/148‑1 (MQ=255)                                                                  gAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGtt                                                                                 >  1:634416/1‑150 (MQ=255)                                                                  gAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGtt                                                                                 >  1:76009/1‑150 (MQ=255)                                                                      atGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATa                                                                             <  1:450348/150‑1 (MQ=255)                                                                          cGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGt                                                                        >  1:675953/1‑151 (MQ=255)                                                                            taataaCCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGttt                                                                      >  2:241993/1‑151 (MQ=255)                                                                             aataaCCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGtttt                                                                     >  1:375846/1‑151 (MQ=255)                                                                              ataaCCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTc                                                                    >  2:27734/1‑151 (MQ=255)                                                                               taaCCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCg                                                                   >  2:267440/1‑151 (MQ=255)                                                                                   cGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTAt                                                                >  2:113444/1‑150 (MQ=255)                                                                                   cGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATg                                                               <  2:174416/151‑1 (MQ=255)                                                                                          tgaatAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCt                                                        >  1:249375/1‑151 (MQ=255)                                                                                               aaaGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGtt                                                   >  2:151644/1‑151 (MQ=255)                                                                                                aaGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTtg                                                  <  2:464917/151‑1 (MQ=255)                                                                                                aaGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTtg                                                  >  1:129605/1‑151 (MQ=255)                                                                                                      tACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTggtggt                                             <  2:316865/150‑1 (MQ=255)                                                                                                         cGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCa                                          >  1:257976/1‑150 (MQ=255)                                                                                                           cAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAAtt                                       <  1:564463/151‑1 (MQ=255)                                                                                                                 atTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCt                                 >  2:387942/1‑151 (MQ=255)                                                                                                                 atTAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCt                                 >  1:417481/1‑151 (MQ=255)                                                                                                                   tAAGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCtt                                >  2:239846/1‑150 (MQ=255)                                                                                                                    aaGAATAGCCTCTAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTa                               >  2:574162/1‑150 (MQ=255)                                                                                                                              tctAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAgg                    >  2:16953/1‑151 (MQ=255)                                                                                                                               ctAAATGATTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAAGTACTCCTTAATGTTCGTAg                     <  2:693142/149‑1 (MQ=255)                                                                                                                                      aTTATGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCAt            <  2:4779/151‑1 (MQ=255)                                                                                                                                         aTGGATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATaaa         <  1:39538/151‑1 (MQ=255)                                                                                                                                           ggATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATaaaaa       <  1:569482/151‑1 (MQ=255)                                                                                                                                           ggATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATaaaaa       <  1:498413/151‑1 (MQ=255)                                                                                                                                           ggATTATTGAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTACTTAATGTTCGTAGGTTAGGCATaaaaa       <  1:409154/151‑1 (MQ=255)                                                                                                                                                attgAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGcc  <  1:639949/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    ACTATTCATAACATTTCAGGATTATCGCCAGATGAAATTGGTGACACTAATACCAGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCC  >  NC_000913/480929‑481222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑