Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,347,882	Δ1 bp	coding (1031/1935 nt)	rnb ←	ribonuclease II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,347,882	0	C	.	100.0%	214.8 / NA	45	coding (1031/1935 nt)	rnb	ribonuclease II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (26/19);  total (26/19)

TTCGACGATACGGTTGGCAATGCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATC  >  NC_000913/1347743‑1348027                                                                                                                                            |                                                                                                                                                  ttcGACGATACGGTTGGCAATGCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTgcgc                                                                                                                                         >  2:599793/1‑149 (MQ=255)  tcGACGATACGGTTGGCAATGCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTa                                                                                                                                       <  2:223245/150‑1 (MQ=255)    gacgaTACGGTTGGCAATCCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGCCGCTGG‑AAATTTGCGCTAGc                                                                                                                                     >  2:410545/1‑150 (MQ=255)        aTACGGTTGGCAATGCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACg                                                                                                                                <  1:398783/151‑1 (MQ=255)         tACGGTTGGCAATGCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAAc                                                                                                                                 <  1:37901/149‑1 (MQ=255)          aCGGTTGGCAATGCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACgg                                                                                                                               >  1:671594/1‑150 (MQ=255)                   aaTGCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCgg                                                                                                                     >  1:28497/1‑151 (MQ=255)                    aTGCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGc                                                                                                                    >  1:699388/1‑151 (MQ=255)                        gacgaGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAAtt                                                                                                                <  1:360412/151‑1 (MQ=255)                        gacgaGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAAtt                                                                                                                >  2:439375/1‑151 (MQ=255)                                  ggCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAACCGGACTTGTTCGGCAATTGCTTCACttt                                                                                                      >  2:89889/1‑151 (MQ=255)                                      acgaTATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAgg                                                                                                  >  1:753032/1‑151 (MQ=255)                                        gaTATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCt                                                                                                >  1:274963/1‑151 (MQ=255)                                        gaTATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCt                                                                                                >  1:99656/1‑151 (MQ=255)                                        gaTATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCt                                                                                                >  1:477005/1‑151 (MQ=255)                                                      cACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTa                                                                                  <  1:405004/151‑1 (MQ=255)                                                         cTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTAttt                                                                               >  2:140597/1‑151 (MQ=255)                                                            tttCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTcc                                                                            >  1:669064/1‑151 (MQ=255)                                                            tttCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTcc                                                                            >  1:588452/1‑151 (MQ=255)                                                              tCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTcc                                                                            >  1:405166/1‑149 (MQ=255)                                                                    agaATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTc                                                                    <  1:169113/151‑1 (MQ=255)                                                                      aaTAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAg                                                                  <  1:462735/151‑1 (MQ=255)                                                                       aTAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTc                                                                    >  1:270640/1‑148 (MQ=255)                                                                           aGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAAcc                                                             >  2:389383/1‑151 (MQ=255)                                                                                tAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTc                                                        >  1:175193/1‑151 (MQ=255)                                                                                 aaTCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCa                                                       <  2:706977/151‑1 (MQ=255)                                                                                            tCTTCAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAgctt                                            >  2:320129/1‑151 (MQ=255)                                                                                             cTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAgcttt                                           >  1:552073/1‑151 (MQ=255)                                                                                               ttAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCCCTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAgctg                                            <  2:247841/148‑2 (MQ=255)                                                                                               ttAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAgctt                                            <  2:526888/148‑1 (MQ=255)                                                                                               ttAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAgc                                              <  1:757029/146‑1 (MQ=255)                                                                                               ttAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAg                                               <  1:589402/145‑1 (MQ=255)                                                                                                    cacCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AACTTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTgg                                    >  2:517941/1‑151 (MQ=255)                                                                                                    cacCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATCGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTgg                                    >  2:43724/1‑151 (MQ=255)                                                                                                       cAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGAtt                                 <  1:608875/151‑1 (MQ=255)                                                                                                        aGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTc                                <  1:410798/151‑1 (MQ=255)                                                                                                           gCGTGGTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGAt                             >  1:155746/1‑151 (MQ=255)                                                                                                               ggTTATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGAtggtg                         <  1:560093/151‑1 (MQ=255)                                                                                                                  tATGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTggcg                      >  1:612327/1‑151 (MQ=255)                                                                                                                   aTGACGCCACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTggcg                      <  2:274910/150‑1 (MQ=255)                                                                                                                         ccACTCGCCGCGGCGTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCGTTGCTTTGGATTCGATGGTGGCGGCAAAGa               <  1:734904/151‑1 (MQ=255)                                                                                                                           aCTCGCCGGGGTGTTGG‑AAATATGCCGTAGCAAACCGACTTGTTCGGGAATTCCTCCCCTTTCAGGCTCCCAGTCGCCGGTATTTTCGAGCCAGTGAGAACCCTAGTCATACACCAGCTTTGCTTTCGATTCGATGGTGGCGTCAAAGaa              <  2:637894/150‑1 (MQ=255)                                                                                                                                   cggcgTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGAtatt     <  1:771838/151‑1 (MQ=255)                                                                                                                                    ggcgTTGG‑AAATCTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGAtatt     >  1:154921/1‑150 (MQ=255)                                                                                                                                      cgTTGG‑AAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATc  >  1:321364/1‑151 (MQ=255)                                                                                                                                            |                                                                                                                                                  TTCGACGATACGGTTGGCAATGCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATC  >  NC_000913/1347743‑1348027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑