| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,725,643 | Δ1 bp | coding (104/1299 nt) | kgtP ← | alpha‑ketoglutarate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,725,642 | 0 | C | . | 97.6% | 186.6 / ‑3.7 | 41 | coding (105/1299 nt) | kgtP | alpha‑ketoglutarate transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (22/18); total (22/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTC > NC_000913/2725492‑2725787 | gCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAAc > 2:657462/1‑151 (MQ=255) cGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAAca < 1:231540/151‑2 (MQ=255) cAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCg > 2:12673/1‑151 (MQ=255) aTAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGACGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGAc > 2:582092/1‑150 (MQ=255) cGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCt > 1:464277/1‑151 (MQ=255) gcATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGccccc < 1:138894/151‑1 (MQ=255) cAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACaa < 1:302623/151‑1 (MQ=255) aaTCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCg > 2:51319/1‑150 (MQ=255) aTCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGcc < 1:51230/151‑1 (MQ=255) aTCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGcc > 2:519024/1‑151 (MQ=255) tCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGccc > 1:509626/1‑151 (MQ=255) ccGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCaa < 1:648845/151‑1 (MQ=255) gcagcaAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAAt < 1:98647/151‑1 (MQ=255) agtagtTGAGTCGTCGTGTTCCCGGAAGGTAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGGGTAGACATAGAAATCGAA‑CACGCGGCCAGACTACCCGAAGAGGCCCCCGCAAACGCCCAAATGCGGCGACGAGTTTCACTACTTg < 2:225684/150‑1 (MQ=255) agtagtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTg < 1:63773/150‑1 (MQ=255) gtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGt > 2:580499/1‑151 (MQ=255) cgtcgtGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTg < 1:62708/150‑1 (MQ=255) gtcgtGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTc > 1:44248/1‑151 (MQ=255) cgtGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCt < 1:156669/150‑1 (MQ=255) cgtGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTg < 1:667612/151‑1 (MQ=255) gtgtTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CGCTCGACCAGATTGCCTGAAGAGGCCCCCACAATCGGCCAAATGCGGCGACGAGTATCGCTACTTGTCAGTTTGCTGTCt < 2:396379/149‑1 (MQ=255) gaagggaagAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGt < 1:305707/151‑1 (MQ=255) gaagaaGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACttt > 2:9506/1‑151 (MQ=255) aagaTGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgc < 2:150251/151‑1 (MQ=255) gaTGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgcc > 1:69496/1‑150 (MQ=255) aTGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgccat > 1:252139/1‑151 (MQ=255) gCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCAtt > 1:535017/1‑151 (MQ=255) aGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGt < 2:551932/151‑1 (MQ=255) gTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGtc < 1:703046/151‑1 (MQ=255) tAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGtct > 2:386879/1‑151 (MQ=255) tGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGc > 2:377389/1‑151 (MQ=255) gAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGc < 2:379545/150‑1 (MQ=255) aGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGCAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTa > 2:439606/1‑150 (MQ=255) aacgatgcgccATAGAAATCG‑ACCACTCTACCAGTTTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCTCCGAACATCTCTACTTGTCAGTGTGCGGTCTGCCGTTACCGTACTTTACGGCAGGCCCTTATGTCACCTGCCGTAAt < 2:278668/140‑1 (MQ=255) cGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCg < 1:232454/151‑1 (MQ=255) aGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGAt > 2:752784/1‑151 (MQ=255) aGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCt > 2:62708/1‑151 (MQ=255) gACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCtt > 2:38249/1‑151 (MQ=255) aCATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCttt > 1:608339/1‑151 (MQ=255) aCATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCttt > 1:728996/1‑151 (MQ=255) cATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCtttt > 1:742283/1‑151 (MQ=255) aTCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTgtcggtc > 2:156454/1‑151 (MQ=255) aTCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTgtcggtc < 1:175457/151‑1 (MQ=255) | GCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTC > NC_000913/2725492‑2725787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |