Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	701,614	IS1 (+) +9 bp	coding (1130‑1138/1149 nt)	nagA ←	N‑acetylglucosamine‑6‑phosphate deacetylase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	257908 =	NA (NA)	46 (0.930)	44/298	0.2	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	701614 =	0 (0.000)					coding (1138/1149 nt)	nagA	N‑acetylglucosamine‑6‑phosphate deacetylase
*	?	NC_000913	= 258675	NA (NA)	50 (1.010)	40/298	0.4	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 701622	0 (0.000)					coding (1130/1149 nt)	nagA	N‑acetylglucosamine‑6‑phosphate deacetylase

AGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/258527‑258675 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCGGCGGTTTATCGCCTGATTGACCAGTACGGGCCAATCTCGCGGATTCAGATTGCCGAG  <  NC_000913/701622‑701475                                                                                                                                                                                                                                                                                                           AGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAC                                                                                                                                                    <  1:187904/151‑1  GAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACG                                                                                                                                                   <  1:573135/151‑1     ATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGG                                                                                                                                                <  2:427911/151‑1      TAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGT                                                                                                                                               >  1:295793/1‑151          gaTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGT                                                                                                                                            >  2:748412/3‑150             GATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAA                                                                                                                                          <  1:289779/149‑1               TGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTC                                                                                                                                       >  2:160329/1‑150                 TCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAAT                                                                                                                                    >  1:763797/1‑151                  CCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAAT                                                                                                                                    >  2:434027/1‑150                   CGGCGGTGCTTGTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACCGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACAACGAGGTCGTAACTCAAT                                                                                                                                    <  2:411910/149‑1                    GGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATA                                                                                                                                   >  1:590225/1‑149                           CTTTTGCCGTTACGCGCCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGGTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAG                                                                                                                           <  2:630744/150‑1                            TTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGT                                                                                                                          >  2:326886/1‑150                               TGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATG                                                                                                                       <  1:336347/150‑1                               TGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATG                                                                                                                       >  1:486750/1‑150                               TGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTAT                                                                                                                        >  1:547657/1‑149                                      acgctttatCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGGCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACAAGG                                                                                                               >  2:296252/10‑151                                      ACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGG                                                                                                               <  1:663423/151‑1                                       CGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGC                                                                                                              <  2:395971/151‑1                                           CCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCGCCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGAC                                                                                                          <  2:5707/151‑1                                               CCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGC                                                                                                      <  2:440161/151‑1                                                CCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCT                                                                                                     >  1:279507/1‑151                                                      GTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATA                                                                                               <  2:428097/151‑1                                                      GTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGAT                                                                                                >  2:585387/1‑150                                                      GTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGAT                                                                                                >  2:105307/1‑150                                                        cGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGG                                                                                             >  2:11289/2‑151                                                               CAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTT                                                                                      >  1:508606/1‑151                                                                AGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTG                                                                                     <  2:414041/151‑1                                                                AGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTG                                                                                     >  2:16916/1‑151                                                                  GAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGAT                                                                                   >  2:356271/1‑151                                                                      GACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCG                                                                               <  1:392219/151‑1                                                                         AGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTA                                                                             >  2:29341/1‑150                                                                             GATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAAC                                                                         <  1:575494/150‑1                                                                                          GCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCG                                                           <  2:48852/151‑1                                                                                           CCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCGG                                                          >  1:663444/1‑151                                                                                           CCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCGG                                                          >  1:61560/1‑151                                                                                           CCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCGG                                                          >  2:529038/1‑151                                                                                            CACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCGGC                                                         >  2:67412/1‑151                                                                                              CTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCGGCGG                                                       >  2:344704/1‑151                                                                                                              ACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCGGCGGTTTATCGCCTGATTG                                        >  1:579561/1‑150                                                                                                                        ACACTAAATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCGGCGGTTTATCGCCTGATTGACCAGTACGG                              <  1:334223/150‑1                                                                                                                               ATCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCGGCGGTTTATCGCCTGATTGACCAGTACGGGCCAATCT                      <  2:551556/151‑1                                                                                                                                TCAGTAAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCGGCGGTTTATCGCCTGATTGACCAGTACGGGCCAATCTC                     >  1:382545/1‑151                                                                                                                                     AAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCGGCGGTTTATCGCCTGATTGACCAGTACGGGCCAATCTCGCGG                 <  1:684354/150‑1                                                                                                                                     AAGTTGGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCGGCGGTTTATCGCCTGATTGACCAGTACGGGCCAATCTCGCGG                 <  2:550892/150‑1                                                                                                                                          GGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCGGCGGTTTATCGCCTGATTGACCAGTACGGGCCAATCTCGCGGATTCA            <  2:154348/150‑1                                                                                                                                          GGCAGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCGGCGGTTTATCGCCTGATTGACCAGTACGGGCCAATCTCGCGGATTCA            <  1:398430/150‑1                                                                                                                                             AGCATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCGGCGGTTTATCGCCTGATTGACCAGTACGGGCCAATCTCGCGGATTCAGATT        <  1:767338/151‑1                                                                                                                                                ATCACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCGGCGGTTTATCGCCTGATTGACCAGTACCGGCCAATCTCGCGGATTCAGATTTCC     >  2:773668/1‑151                                                                                                                                                   ACCACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCGGCGGTTTATCGCCTGATTGACCAGTACGGGCCAATCTCGCGGATTCAGATTGCCGAG  <  1:66204/151‑1                                                                                                                                                                                                                                                                                                           AGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/258527‑258675 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ACGAGGTCGTAACTCAATAAGAGAAAGTATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCGGCGGTTTATCGCCTGATTGACCAGTACGGGCCAATCTCGCGGATTCAGATTGCCGAG  <  NC_000913/701622‑701475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.