Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,157,282	0	A	G	40.0%	26.7 / 10.4	31	D169G (GAT→GGT)	ycfH	putative DNase
Reads supporting (aligned to +/- strand):  ref base A (6/12);  new base G (12/0);  total (19/12)
Fisher's exact test for biased strand distribution p-value = 3.34e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGC  >  NC_000913/1157133‑1157416                                                                                                                                                       |                                                                                                                                       tGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGa                                                                                                                                        <  2:127411/150‑1 (MQ=255)  ggTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGAt                                                                                                                                       <  2:565831/150‑1 (MQ=255)  ggTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGAt                                                                                                                                       <  1:242484/150‑1 (MQ=255)     cGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGAtctc                                                                                                                                    <  2:126562/150‑1 (MQ=255)                cAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAAATAATGGAGCTCgggtgtt                                                                                                                             >  2:344047/1‑141 (MQ=255)                 aaGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAATTAATGGGTCTCGGGtttt                                                                                                                             >  2:758540/1‑145 (MQ=255)                    ccGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCt                                                                                                                    <  2:21903/151‑1 (MQ=255)                        ttATCGTCCATACCTGTGACCCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACTGTGAAACGGC‑GGGTAAATGACTGGATCTCGGATTTTACAtcttct                                                                                                                    <  2:2767/147‑1 (MQ=255)                           tCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAAATAATGGGTCTCGGGTTTTAAATCTCCtttt                                                                                                                 >  1:649551/1‑147 (MQ=255)                                   aCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAATTACTAGGTTGCGGGTTTTAAATATTCtttt                                                                                                                 >  2:517699/1‑139 (MQ=255)                                              ccGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAAt                                                                                          <  2:384787/151‑1 (MQ=255)                                                    cGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGGGAATAAATGGGTGTTGGGTTTTTAAACCTCTTTTTTGGCCTTGGGTCCTTTCGTAATggg                                                                                       >  2:688874/1‑146 (MQ=255)                                                     gATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAAATATTTGGTCTCGGATTTTACAACTCCTTTTCCGGCATTTTGACCCTCCGTAATGccggg                                                                                    >  1:486857/1‑146 (MQ=255)                                                          gCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAGATTACTGGGTGTCTGGTTTTTCATCTCCTTTTTCGCGCTTGTGACCATCCGGaaagag                                                                                       >  2:307284/1‑138 (MQ=255)                                                            tGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAATTACTGGAACTCGGGTTTTACATCTCCTTTTTCCGCATTTTTAGCTTCCGTAATGCGGGGGAACTg                                                                              >  1:254269/1‑149 (MQ=255)                                                             ggCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTgcgc                                                                           <  1:617506/151‑1 (MQ=255)                                                                      gcgcGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGAtgctg                                                                    <  1:470638/149‑1 (MQ=255)                                                                                aaaGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTcc                                                        <  1:493901/151‑1 (MQ=255)                                                                                   gTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAATTACTGGGTTTCGGATTTTAAATCTCCTTTTTCGGCATTGTGACCTTTCGTAATGCGGAGCAACTGCGCGATGCTGCGCGGTATGTccccc                                                     >  1:170496/1‑151 (MQ=255)                                                                                    tGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAATTACTGGGGTTCGGGTTTTAAATCTCCTTTTTCGGCATTTTGACCTTCCGTAAAGCGGGGCAACTGCGCGATGCTGCGCGGTATGTccccc                                                     >  1:122845/1‑150 (MQ=255)                                                                                                      tactacACTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAATTACTGGGTATCCGGTTTTAAAATTCCTTTTTCCGCCTTGTGACCTTCCCTAATTCGGAGCAACTGCGCGGTGCTGCGCGGTATGTTCCCCCGGGTCGGTTTCtggggg                                   >  1:554479/1‑150 (MQ=255)                                                                                                        ctacACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTTGGTCTCGGATTTTACATCTCCTTTTTCGGCATTGTGACCTTCCGTAATGCGGGGCAACTGCGCGGTGCTGCGCGTTATGTCCCCCCGGGGCGGGTACtggtgg                                   >  1:195793/1‑148 (MQ=255)                                                                                                         tacACTGTTTTACAGAGGACAGAGAAACGGCGGGGGAAATTA‑TAGTTCTCTGATTTTAAATCTTCTTTTTCGGCGTTGTGAACTTCCGTAATGCGGGGGAACTTCGCGATGCTGCGCGTTTTGTTCCCCTCGAGCgtggtctggtgg                                   >  2:254931/1‑136 (MQ=255)                                                                                                          acacTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAAATAATGGAGCTCTGGATTTAAAAATCCTTTTTCGGCCGTTTGACCTTCCGCAATACGGGGCAACTGCCCGATTCTGCGCGTTATGTCCCCCTGGATCGGTGATtggggg                                   >  2:748693/1‑146 (MQ=255)                                                                                                               gTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACt                          <  2:97257/150‑1 (MQ=255)                                                                                                               gTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTAATTGGGATCGGAGTTTAAATCTCCTTTTTCGGGATTGTGTCCCTCCCTAATGCGGAGCAACTACGCGATTCTGCGCGTTTTTTCCCCCCGGGTCGGTTACttggggga                                 >  2:114243/1‑136 (MQ=255)                                                                                                                 tttACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCa                        <  2:486576/150‑1 (MQ=255)                                                                                                                    aCAGAGGACAGAGAAACGGC‑GGGGAAATTACTTGGTCTCGGGTTTTTAAACTCCTTTTTCGGCATTGTGACCTTCCGTAATTCGGGGCAACTGCGCGATGCTGCGCGTTTTTTTCCCCTCGGTCGGGTAATGGGGGAAAAAGACTCacct                     >  2:635798/1‑150 (MQ=255)                                                                                                                         ggACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCaccttacctt               <  1:719878/151‑1 (MQ=255)                                                                                                                         ggACAGAGAAACGGC‑GGGGAAATTACTGGATCTCGGATTTTAAATCTCCTTTTTCGGCATTGTGACCTTCCGTCATGCGGGGCAACTTCGCGCTGCTGCGCGTTTTGTCCCCCTGGATCGGGGACTGGGGGGAACTGACTCaccttacc                 >  2:235334/1‑149 (MQ=255)                                                                                                                                     ggc‑ggGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGGGGGAACTGACTCACCTTACCTTGCGCCGGGACCg   >  2:292143/1‑151 (MQ=255)                                                                                                                                       c‑ggGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGc  >  2:657938/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                       TGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGC  >  NC_000913/1157133‑1157416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑