Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,349,1450AC40.5% 71.7 / 11.2 42F110V (TTC→GTC) inaAacid‑inducible Kdo/WaaP family putative kinase
Reads supporting (aligned to +/- strand):  ref base A (23/2);  new base C (0/17);  total (23/19)
Fisher's exact test for biased strand distribution p-value = 6.71e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATGCTGACGATTAATGCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTCAACATGGCT‑TGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACG  >  NC_000913/2349018‑2349294
                                                                                                                                |                                                                                                                                                     
ccATGCTGACGATTAATGCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTCAACATGGCT‑TGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCAcc                                                                                                                                 >  2:28938/1‑150 (MQ=255)
ccATGCTGACGATTAATGCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTCAACATGGCT‑TGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCAcc                                                                                                                                 >  1:657087/1‑150 (MQ=255)
         ctattattTCTATGCTTTTTCTAAAACCCCAGCGCCACTTCTTCCAACGTGTTT‑TGCCGTTTTTCATAAGAAAAGGGCGATACTGCATGCTGGCAATACCAGACAGCAATGTGGAGAACCCCCGCCATATCTTCAGTCACCAGCAAc                                                                                                                           <  1:23591/145‑1 (MQ=255)
           attattGCTATGTTTTTTTTAAACCCCCCGCCCCCCTTCTTACAACTTGGTT‑TCCCGTCCTTCTTAAGAAAAAGGCAATCCTGCTTGCGGGTCAACCCAGTAAGAAATGGTAATGACCCCCGCCATATCTTCAGTCACCAGCAACGCTc                                                                                                                       <  1:539187/149‑1 (MQ=255)
           aTTAATGCTATTCATTTTATTAACCGCCCGCCCCCCTGCTTTCAACA‑GGCTGTGCTTTTCTTCGTCATAATAAGCCCACCCGCCTTGGTGGCCATCCCGATCAGCAATGCTAATGACCCCCGCCATATCTTCAGTCACCAGCAACGCTc                                                                                                                       <  2:715781/149‑1 (MQ=255)
            ttAATGCTATGCTTTTTCTAAAACGCCGGCGCCCCTGCTTTCAACATGGTT‑TGCCGTACTTCGTCAAAATAAGGCGATACTGCATGCGGGCCATACCAGTCAGCAATGCTGATGACCCCCGCCATATCTTCAGTCACCAGCAACGCTCg                                                                                                                      <  1:742646/149‑1 (MQ=255)
             tAATGCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTCAACATGGCT‑TGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGcc                                                                                                                    >  1:315592/1‑150 (MQ=255)
               tcccgattttttttCTAAACCGCCAGCCCCCCTGCTTTCAACATGGCT‑TCCCGTACTTCGTAAAAAAAAGGCGATCCTGCATGCTGGGCATCCCAGTCAGCAATGCTGATAACCCCCGCCATATCTTCAGTCACCAGCAACGCTc                                                                                                                       <  1:769280/135‑1 (MQ=255)
                tGCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTCAACATGGCT‑TGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTc                                                                                                                >  2:289478/1‑151 (MQ=255)
                 gCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTCAACATGGCT‑TGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTc                                                                                                                >  2:9372/1‑150 (MQ=255)
                 gCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTCAACATGGCT‑TGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTc                                                                                                                >  2:45878/1‑150 (MQ=255)
                   tATGCATTTTCTTAAACGCCAGCGCCACTGCTTTCAACATGGCT‑TGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCAcc                                                                                                             >  2:422785/1‑151 (MQ=255)
                        aTTTTCTTAAACGCCAGCGCCACTGCTTTCAACATGGCT‑TGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTc                                                                                                                       >  2:768912/1‑136 (MQ=255)
                         ttttttAAACCGCGAGCGCCACTGCTTAAAACGGGGCT‑GGCCTTCCTACGTCAGAATAAGGCGATACGGCATGCTGGCAACCCCAGTCACCAAGGCTAATTACCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTc                                                                                                          <  2:122369/148‑1 (MQ=255)
                           ttCTTAAACGCCAGCGCCACTGCTTTCAACATGGCT‑TGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAAttt                                                                                                     >  2:761085/1‑151 (MQ=255)
                                 aaCGCCAGCGCCACTGCTTTCAACATGGCT‑TGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCg                                                                                               >  1:327705/1‑151 (MQ=255)
                                 aaCGCCAGCGCCACTGCTTTCAACATGGCT‑TGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCg                                                                                               >  1:534044/1‑151 (MQ=255)
                                  aCGCCAGCGCCACTGCTTTCAACATGGCT‑TGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGc                                                                                              >  1:509150/1‑151 (MQ=255)
                                   cGCCAGCGCCACTGCTTTCAACATGGCT‑TGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCt                                                                                             >  2:563933/1‑151 (MQ=255)
                                      cAGCGCCACTGCTTTCAACATGGCT‑TGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTc                                                                                           >  2:469351/1‑150 (MQ=255)
                                         ccccccTGTTTTAAACAGGGCT‑GGCCGTCCTTCGACAAAAAAAGGGGATCCTGCATGCGGGGCACACCAGTCAGCAATGCTGATAACCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCtt                                                                                            <  1:320458/141‑1 (MQ=255)
                                               tccTTCCAACACGCCT‑TGCCTTACTCCATAAGAAAAAGGCGATACTGAAGCCGGGGCATCCCCGCAAACACGGCTAATAAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCaaaa                                                                                    <  1:692280/146‑1 (MQ=255)
                                                  tttCAACATGGCT‑TGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATc                                                                              >  2:583783/1‑151 (MQ=255)
                                                   ttCAACGTGGTT‑TGCCGTACTTCGTAAAAATAAGGCGATACTGCATGCGGGGCATCCCAGTCAGAAATGCTGATGACCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATc                                                                              <  1:516376/150‑1 (MQ=255)
                                                               tgccttttcTTCGCCAAAATAAGGCGTTCCGCCATGCTGGCCCTCCCAGCCACAACTGCTGATACCCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCAc                                                                      <  2:604936/139‑1 (MQ=255)
                                                                    gTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGacac                                                              >  2:467113/1‑150 (MQ=255)
                                                                      aCTTCGTAAAAATAAGGCGATCCTGCATGGGGGGCATACCAGTCAGCAATGCTGATAACCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCg                                                            <  1:254501/150‑1 (MQ=255)
                                                                       cTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGc                                                          >  2:514328/1‑151 (MQ=255)
                                                                          cgtaaaaaaagggCGATACTGCATGCTGGGCATCCCAGTCAGAAATGCTGATGACCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGccc                                                        <  1:319503/139‑1 (MQ=255)
                                                                           gacAGAAAAGGGCGATACTGCATGCGGGGCATCCCAGCCAGCAATGCTGATAACCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACAcc                                                             <  1:350730/142‑1 (MQ=255)
                                                                            acaaaaaaaGGCGTTCCTCCATGCTGGCCATCCAAGTCACAAATGCTGATCACCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGccc                                                        <  2:583208/141‑1 (MQ=255)
                                                                              aGAAAAAGGCACTACGGCATGCGGGCCACCCAAGCCACCATTGCGGATCACCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGccc                                                        <  2:733695/146‑1 (MQ=255)
                                                                               gAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCACTTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTcc                                                   >  2:558855/1‑150 (MQ=255)
                                                                                      gCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCttt                                           >  1:104125/1‑151 (MQ=255)
                                                                                         aTACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAAt                                        >  1:17563/1‑151 (MQ=255)
                                                                                             tGCATGCTGGGCATACCAGTCAGCATTGCTGATGACCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAAtt                                       <  1:395927/148‑1 (MQ=255)
                                                                                                  gCTGGCCATACCATTCAGCACTGTTAATGACCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCg                                   <  2:377132/147‑1 (MQ=255)
                                                                                                    tGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCt                             >  2:230728/1‑151 (MQ=255)
                                                                                                    tGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCt                             >  1:679717/1‑151 (MQ=255)
                                                                                                    tGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCt                             >  1:248827/1‑151 (MQ=255)
                                                                                                              aggcagcagtcttgatgcCCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTCACGGACa                     <  2:697997/137‑1 (MQ=255)
                                                                                                                     aaggCTGATGAATCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCg                 <  1:720044/143‑1 (MQ=255)
                                                                                                                               aaCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACg  >  2:373774/1‑151 (MQ=255)
                                                                                                                                |                                                                                                                                                     
CCATGCTGACGATTAATGCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTCAACATGGCT‑TGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACG  >  NC_000913/2349018‑2349294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑