Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,483,268	0	T	A	41.3%	76.1 / 28.3	64	L24F (TTA→TTT)	emrK	multidrug resistance efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base T (20/17);  new base A (0/26);  total (20/44)
Fisher's exact test for biased strand distribution p-value = 1.26e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGC  >  NC_000913/2483125‑2483417                                                                                                                                                |                                                                                                                                                      tCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACt                                                                                                                                                 >  1:198999/1‑150 (MQ=255)    ttATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTAc                                                                                                                                               <  1:525488/149‑1 (MQ=255)    ttATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCg                                                                                                                                             >  1:168784/1‑151 (MQ=255)      aTGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGc                                                                                                                                            <  2:482221/150‑1 (MQ=255)        gATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCa                                                                                                                                          <  1:230982/150‑1 (MQ=255)                  aGTGACACTCCCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATGGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCCCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGaaaa                                                                                                                                <  1:502964/150‑1 (MQ=255)                  aGTGACACTACCTGAGACTGGTGCAGAAATTGGTTCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATCCCATTGACAAAAAGGAATAGGCACCTGAAAACGCAAAAAAAAAAACTCCCGCCAATAAAGAAAAg                                                                                                                               <  2:781495/151‑1 (MQ=255)                      acacTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTAtt                                                                                                                           >  2:253700/1‑151 (MQ=255)                       cacTACCTGAGATTTGTGCAAAAATTGGATCTGCATTCCCCGTGACATAGGCGTAATCTGTACTAAACATGTCTTCAAATCCCATTGCCAAAAAGGAAAAGGCACCGAAAAACGCAAAAAAAAAAACTACCGCCAATAAAGAAAAg                                                                                                                               <  2:113222/146‑1 (MQ=255)                         ctcccTGAGACTTGTGCAGAAATTGGATCTCCATTCCCCGGGACATAGGCGTCATCTGTACAAATCATTTCTTCAAATCCCATTGACCAATAGAAATAGGCCCCTAAAAACGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTAtttt                                                                                                                         <  2:1002840/147‑1 (MQ=255)                          tACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttct                                                                                                                       >  2:1017397/1‑151 (MQ=255)                            ccTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttc                                                                                                                        <  1:829764/148‑1 (MQ=255)                             cTGAGATTTGTGCAAAAATTGGATTTGCATTCCCCGTGACATAGGCGTCATCTGTACAAATCATTTCTTCTAATCCCATTGACAAAAAGGAATAGGCACTTAAAAACACAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTttcttc                                                                                                                     <  2:190666/150‑1 (MQ=255)                                agttttGTGCAGAAATTGGATCTGCATCCCCCGTGACAAAGGCGTCATCTTTACTAATCATGTTTTCTAATTCCATTGCCCAAAGGGCATAGGCCCCTGAAACCGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGtt                                                                                                                 <  2:540263/147‑1 (MQ=255)                                agaTTTGTGAAAAAATTGGATCTGCATCCCCCGTAACATAGGGTTCTTCTGTACAAATCATTTCTTCAAATTCCATTGACCAAAAGGCATAGGCCCCTAAAACCGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTtcttct                                                                                                                    <  1:521288/148‑1 (MQ=255)                                    ttGTGAAAAAATTGGTTCTGCATTCCCCGTGACAAGGGCGTCATCTGTACTAATCATGTCTTCAAATCCCATTGACCAATAGAAAAAGCCACCTGAAACCGCAAAAAAAAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAg                                                                                                               <  2:708006/149‑1 (MQ=255)                                     tgtgCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAAt                                                                                                            >  2:582446/1‑151 (MQ=255)                                     tgtgCAAAAATTGAATCTGCATTCCCCGTAACATAGGCGCCATCTTTACAAATCATGTCTTAAAATTCCATTGCCCAATAGGAATAGGCCCCTGAAAACGTAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGaa                                                                                                             <  2:775205/150‑1 (MQ=255)                                       tgaaGAAATTGGATCTGCATCCCCCGTGACATAGGCGTCATCTGTACTAATCATTTCTTCTAATTCCATTGACAAATAGGCATAGGCACCTAAAACCGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGaa                                                                                                             <  1:264616/145‑1 (MQ=255)                                       tgCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATTTTTTCTAATTCCATTGACCAAGAGACATAGCCCCCTGAAACCGTAATAAAAAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATg                                                                                                           <  2:300826/150‑1 (MQ=255)                                       tgCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAAACATGTCTTCTAATTCCATTGCCAAAAAGGCAAAGGCACCTGAAAACGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAAt                                                                                                            <  2:489220/149‑1 (MQ=255)                                        gCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtt                                                                                                         >  2:277866/1‑151 (MQ=255)                                          aGAAATTGGATCTGCATTCACCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGGATTTTCTTCTGTTAGAATGtttt                                                                                                       >  2:23957/1‑151 (MQ=255)                                            aaaTTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATTTCTTCTAATCCCATTGACCAAAAGGAATAGGCACCTGAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGttttt                                                                                                      <  2:415903/150‑1 (MQ=255)                                                     cTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAtt                                                                                            >  1:857549/1‑151 (MQ=255)                                                            ccccGTGACATGGGCGTCTTCTGTACTAATCATTTCTTCTAATCCCATTGACCAATAGGCAGAGCCCCCTGAAACCGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTa                                                                                           <  1:857632/145‑1 (MQ=255)                                                            ccccGTGACATGGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGt                                                                                     <  1:997455/151‑1 (MQ=255)                                                            ccccGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGt                                                                                     >  2:837768/1‑151 (MQ=255)                                                            ccccGTGACATAGGCGTCATCTGTACTAATCATGTCTTAAAATCCCATTGCCCAATAGGCAAAGGCACCTGAAAACGCAATAAAAAAATCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATc                                                                                        <  2:434313/148‑1 (MQ=255)                                                            ccccGTGACAAAGGCGTCATTTTTACTAATCATGTCTTCTAATCCCATTGACAAATAGGAATAGGCCCCTAAAAACGCAAAAAAAAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGt                                                                                     <  2:575795/151‑1 (MQ=255)                                                             cccGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGtt                                                                                    <  2:434493/151‑1 (MQ=255)                                                                gTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTcc                                                                                  >  2:421748/1‑150 (MQ=255)                                                                       aggcgtctcttttattAATCATTTATTTAAATTCCATTAACCAAAAGGAATAGCCACCTGAAAACGGAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtattat                                                                          <  2:625750/136‑1 (MQ=255)                                                                       aGGCGTCATCTTTACAAATCATTTCTTCAAATTCCATTGCCCAAAAGGCATAGGCACCTTAAACCGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtat                                                                             <  2:816541/148‑1 (MQ=255)                                                                        ggCGTCATCTGTACTAATCATGTCTTCAAATTCCATTGACCAATAGGCATAGGCCCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTCTATTTGAATTAATCTGTTCCACtatta                                                                           <  1:337402/149‑1 (MQ=255)                                                                        ggCGTCATCTGTACTAATAATGTCTTCAAATTCCATTGACAAATAGGCAAAGGCACCTAAAAACTAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtatta                                                                           <  2:277502/149‑1 (MQ=255)                                                                        ggCGCCATCTGTACTAATCATGTTTCCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGAAATAAATAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtat                                                                             <  2:555934/147‑1 (MQ=255)                                                                         gCGTCATCTGTACTAATCATGTCTTCTAATTCCATTCACCAATAGGAATAGGCACCTAAAAACAAAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtct                                                                        <  2:279023/151‑1 (MQ=255)                                                                          cGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtctc                                                                       >  2:1026849/1‑151 (MQ=255)                                                                                       aaTCATGTCTTCTAATTCCATTGACAAAGAGGCATAGGCACCTGAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATa                                                           <  1:997613/150‑1 (MQ=255)                                                                                        aTCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCAt                                                            >  1:817194/1‑148 (MQ=255)                                                                                        aTCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTAAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCAt                                                            <  1:558013/148‑1 (MQ=255)                                                                                          cATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGAt                                                        >  2:568714/1‑150 (MQ=255)                                                                                              tcttctAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGaaa                                                    <  1:768462/150‑1 (MQ=255)                                                                                                   tAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAAtt                                                  <  1:190227/147‑1 (MQ=255)                                                                                                   tAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtg                                              >  1:77531/1‑151 (MQ=255)                                                                                                    aaTTCCATTGACCAATAGAAATAGGCACCTGAAACCGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAt                                               <  2:198828/149‑1 (MQ=255)                                                                                                         cATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattg                                         >  2:522230/1‑150 (MQ=255)                                                                                                          aTTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattga                                        >  1:616026/1‑150 (MQ=255)                                                                                                          aTTGACCAATAGGCATAGGCACCTGAAAACGAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattga                                        <  1:606783/150‑1 (MQ=255)                                                                                                          aTTGACCAATAGGCATAGGCACCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaatt                                          <  1:1027204/148‑1 (MQ=255)                                                                                                           ttGACCAATAGTCATAGCCCCCTAAAAACGAAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaatt                                          <  2:878260/147‑1 (MQ=255)                                                                                                            tgcccAATAGGAATAGCCACCTAAAAACGAAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAGTTTAtgaattgaat                                      <  2:1029712/147‑1 (MQ=255)                                                                                                               ccAATAGGCAGAGGCACCTAAAAACGAAAAAAAGAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatg                                     <  2:1039008/148‑1 (MQ=255)                                                                                                               caaaaaggaataggcaCCTAAAAACCCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattgaat                                      <  2:669966/142‑1 (MQ=255)                                                                                                                  ataggcataggcACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGaaa                                  >  1:749174/1‑148 (MQ=255)                                                                                                                              aCCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttct                    <  1:493028/150‑1 (MQ=255)                                                                                                                                 tGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCat                >  2:484183/1‑151 (MQ=255)                                                                                                                                   aaaaCGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCatat              <  1:871474/151‑1 (MQ=255)                                                                                                                                   aaaaCGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCata               <  1:1040500/150‑1 (MQ=255)                                                                                                                                   aaaaCGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCata               <  1:421979/150‑1 (MQ=255)                                                                                                                                   aaaaCGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttctt                   <  2:437796/146‑1 (MQ=255)                                                                                                                                       cgtaataaataAAACTACAGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGATTTTTATTTGAATTAATCTGGTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAACGAAAAGAGCATTCATTCTTCatat              >  2:1005567/4‑147 (MQ=255)                                                                                                                                       cGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCaa           >  1:84355/1‑150 (MQ=255)                                                                                                                                        gCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCaaa          >  2:784095/1‑150 (MQ=255)                                                                                                                                          aataaataAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAacac       >  2:777279/1‑151 (MQ=255)                                                                                                                                            taaataaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAacac       >  2:828010/1‑149 (MQ=255)                                                                                                                                             aaataaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAg     <  2:652036/150‑1 (MQ=255)                                                                                                                                               ataaaACTACCGCCAATAAAAAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGc  >  1:795765/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                      TCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGC  >  NC_000913/2483125‑2483417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑