Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	599,576	0	T	G	54.9%	45.1 / 82.5	51	V288G (GTG→GGG)	cusA	copper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base T (1/22);  new base G (28/0);  total (29/22)
Fisher's exact test for biased strand distribution p-value = 1.47e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-11

AAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACC  >  NC_000913/599426‑599725                                                                                                                                                       |                                                                                                                                                      aaaCGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGggt                                                                                                                                                       <  2:819059/151‑1 (MQ=255)   aCGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGgggggg                                                                                                                                                     >  1:563088/1‑151 (MQ=255)        cgacgaCTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATc                                                                                                                                                <  2:479351/151‑1 (MQ=255)           cgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGATcc                                                                                                                                               >  1:271170/1‑149 (MQ=255)           cgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGATcc                                                                                                                                               >  1:972292/1‑149 (MQ=255)            gacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATcc                                                                                                                                               >  1:273655/1‑148 (MQ=255)                ttAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGATGCtt                                                                                                                                             >  2:36613/1‑145 (MQ=255)                 tAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATc                                                                                                                                        <  1:397432/150‑1 (MQ=255)                 tAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATcc                                                                                                                                       >  1:347363/1‑151 (MQ=255)                   aTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCgg                                                                                                                                     <  2:164414/151‑1 (MQ=255)                     cacaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCa                                                                                                                                   <  1:996161/151‑1 (MQ=255)                      acaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCCGCGAGcccg                                                                                                                                     >  1:60606/1‑146 (MQ=255)                      acaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCaa                                                                                                                                  <  1:582449/151‑1 (MQ=255)                       caTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGTCCgg                                                                                                                                     >  2:211219/1‑147 (MQ=255)                        aTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCaaaa                                                                                                                                <  2:537210/151‑1 (MQ=255)                          cGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCaaaaa                                                                                                                               >  1:874324/1‑150 (MQ=255)                            ttttAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCGGCaaaaa                                                                                                                               >  1:40985/1‑148 (MQ=255)                                 aaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGGTCCGGGaaaaaaaccc                                                                                                                          >  2:818641/1‑143 (MQ=255)                                   aGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGaa                                                                                                                     <  1:66219/151‑1 (MQ=255)                                          gAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGGCCCGGGATCCGGCAAAAAAGCCCGAGGAGTgg                                                                                                                 >  2:707308/1‑147 (MQ=255)                                            aaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGGGCCTGCGATCCCGGCAAAACGCACGCGGAGGGATcgcc                                                                                                            >  2:617159/1‑151 (MQ=255)                                             aaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATcgccg                                                                                                           <  2:1012927/151‑1 (MQ=255)                                               tGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCGGCAAAAACGCCCCAGAAGGGATCgccgcc                                                                                                         >  2:442795/1‑151 (MQ=255)                                                ggCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGTGAGTCTGCGGTCCGGCCAAAACGCCCGAGAAGGGACcgcccc                                                                                                          >  2:646183/1‑147 (MQ=255)                                                  cGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTg                                                                                                      <  2:417701/151‑1 (MQ=255)                                                      cccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAgg                                                                                                  <  1:348657/151‑1 (MQ=255)                                                       ccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATTCGGCAAAAAAGCCCGGGAAGGGGTCGGCGCCGGGAAgg                                                                                                  >  2:487487/1‑150 (MQ=255)                                                        cGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGGTCCGGCCAAAAAGCCCGAGAAGGGATCGCCGCCGTGGAgg                                                                                                  >  2:1046150/1‑149 (MQ=255)                                                          tttATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGCTCCCGCAAAAACGCCCCAGAAGTGATCGGCGCCGTGAAg                                                                                                   >  2:154655/1‑146 (MQ=255)                                                            tATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCCATCGCGGAAAAACGCCCCAGAAGGGGCTGCCCCCGTGAAGGGCaaa                                                                                             >  1:575458/1‑150 (MQ=255)                                                                 gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGa                                                                                        <  1:1046510/150‑1 (MQ=255)                                                                  cgcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGGTCCTGCGATCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGTGAAGGACAAAATGGaa                                                                                       >  1:910219/1‑150 (MQ=255)                                                                   gcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCCGCGATCCCGCAAAAAAGCCCGAGAAGGGATGGCCGCCGCGGAGGGCAAAACGGaaa                                                                                      >  2:830976/1‑150 (MQ=255)                                                                    cgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACg                                                                                    <  1:793380/151‑1 (MQ=255)                                                                     gATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGc                                                                                   <  2:101270/151‑1 (MQ=255)                                                                              aaGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCCGCGGCCCGGCAAAAAAGCCCGGGGAAGGAGCGCCCCCGTGAAGGACAAACAGGCAACGCTTAAAag                                                                            >  1:481213/1‑149 (MQ=255)                                                                                   ccAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTg                                                                     <  2:126329/151‑1 (MQ=255)                                                                                   ccAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTg                                                                     <  1:790512/151‑1 (MQ=255)                                                                                   ccAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTg                                                                     <  2:396040/151‑1 (MQ=255)                                                                                   ccAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTg                                                                     <  1:229370/151‑1 (MQ=255)                                                                                            cccGGGGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTAAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGc                                                            <  2:310577/151‑1 (MQ=255)                                                                                                  gaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGCTCCTGCGATCCGGCCAAAACGCCCCCGGAGGGATCGCCGCCGTGAAGGACAAACTGGCAACGCTGAAAAGCAGTCTGCCGGAAGGCGTgg                                                        >  2:534647/1‑149 (MQ=255)                                                                                                         cGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGAACCGGCAAAAAAGCCCGGGAAGGGATCGCCGCCGTGGGGGACAAACTGGAAACGCTGAAAAGGAAGCTGCCGGGAGGCGTGGGGATAg                                                  >  2:106375/1‑148 (MQ=255)                                                                                                          ggggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTac                                              <  2:449943/151‑1 (MQ=255)                                                                                                                  gCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAAAGCCCGAGAAGTGATCGCCGCCGGGGAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGGAGGCGTGGAGATAGtttaaac                                           >  1:385464/1‑141 (MQ=255)                                                                                                                   ccGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGa                                     <  1:182756/151‑1 (MQ=255)                                                                                                                     gAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATc                                   <  1:741460/151‑1 (MQ=255)                                                                                                                     gAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGGGATCCTGCGATCCGGCAAAAAAGCCCGAGAAGGGATCGCCGCCGGGAAGGACAAACTGGAAACGCTGAAAAGGAGTCTGCCGGAAGGCGGGGGGATAGTTACAACATACg                                      >  1:727366/1‑148 (MQ=255)                                                                                                                     gAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGGTCCTGCGATCCGGCCAAAACGCCCCAGGAGGGATAGCCGCCGCGAAGGAGAAAATGGAAAAACTGAAAAGTAGTTTGGCGGGAAGCGTGGGGATAGTTacaacaa                                         >  2:842637/1‑144 (MQ=255)                                                                                                                            aCGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAAAGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAAAGCTGAAAAGTAGTCTGCCGGAAGGCGGGGGGATAGTTACAACATACGATCGcagcc                             >  1:662648/1‑150 (MQ=255)                                                                                                                                  aaggcgaagTGGCGGGCGGGGGGGGGATCCTGCGATTCGGCAAAAAAGCCCGAGAAGTGATCGCCCCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGGAGGCGGGGGGATAGTTACAAAATACGGTCGcagccaccgca                       >  2:902384/1‑145 (MQ=255)                                                                                                                                    ggcgaagTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGTGAAGGGCAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGGGGGGAGATAGTTACAACATACGATCGCAGCCAGCTCAtt                     >  1:916413/1‑150 (MQ=255)                                                                                                                                       gaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGAcc                 <  2:1048136/151‑1 (MQ=255)                                                                                                                                                      gtggtgATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAAcc  <  2:202604/151‑1 (MQ=255)                                                                                                                                                      gtggtgATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAAcc  >  1:346878/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                      AAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACC  >  NC_000913/599426‑599725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑