breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsqtrim-GLUT2-10-rerun_S13_L001_R1_0011,069,626160,939,080100.0%150.5 bases151 bases99.7%
errorsqtrim-GLUT2-10-rerun_S13_L001_R2_0011,069,257160,689,063100.0%150.3 bases151 bases98.3%
total2,138,883321,628,143100.0%150.4 bases151 bases99.0%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0009134,641,65268.61.398.3%Escherichia coli str. K-12 substr. MG1655, complete genome.
total4,641,652100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 100000924
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 500028
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.002

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.80493

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.8
Consensus minimum coverage each strandOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum coverage each strandOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Execution Times

stepstartendelapsed
Read and reference sequence file input23:59:25 02 Jan 201723:59:56 02 Jan 201731 seconds
Read alignment to reference genome23:59:57 02 Jan 201700:03:59 03 Jan 20174 minutes 2 seconds
Preprocessing alignments for candidate junction identification00:03:59 03 Jan 201700:04:53 03 Jan 201754 seconds
Preliminary analysis of coverage distribution00:04:53 03 Jan 201700:06:31 03 Jan 20171 minute 38 seconds
Identifying junction candidates00:06:31 03 Jan 201700:06:31 03 Jan 20170 seconds
Re-alignment to junction candidates00:06:31 03 Jan 201700:07:26 03 Jan 201755 seconds
Resolving alignments with junction candidates00:07:26 03 Jan 201700:08:58 03 Jan 20171 minute 32 seconds
Creating BAM files00:08:58 03 Jan 201700:10:13 03 Jan 20171 minute 15 seconds
Tabulating error counts00:10:13 03 Jan 201700:11:38 03 Jan 20171 minute 25 seconds
Re-calibrating base error rates00:11:38 03 Jan 201700:11:38 03 Jan 20170 seconds
Examining read alignment evidence00:11:38 03 Jan 201700:22:30 03 Jan 201710 minutes 52 seconds
Polymorphism statistics00:22:30 03 Jan 201700:22:31 03 Jan 20171 second
Output00:22:31 03 Jan 201700:22:45 03 Jan 201714 seconds
Total 23 minutes 19 seconds