Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,443,2770AC46.2% 48.1 / 15.0 39V274G (GTA→GGA) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (19/2);  new base C (0/18);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 3.35e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACT  >  NC_000913/3443137‑3443420
                                                                                                                                            |                                                                                                                                               
agagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCat                                                                                                                                       >  1:350904/1‑151 (MQ=255)
 gagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCat                                                                                                                                       >  1:566690/1‑150 (MQ=255)
  agTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCatat                                                                                                                                     <  1:243924/151‑1 (MQ=255)
           tAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTc                                                                                                                             >  2:647995/1‑150 (MQ=255)
                      cccggccgaaaaaTACAGCAAATTTTTTGTCAGCCAGTCCCAACCAGCCCCGCCCCCGAACCATGACGCGAGGGTCGCGGGAAAAAAAAAAATCCTGGAAGCGAAGATGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                  <  1:445331/140‑1 (MQ=255)
                      cccAGGCTAAAAAAACAGCAAATTTTTTGTCAGCCGTTCCCAACCAGTCCCGCCCCCAACCCATGGCGCGATGGTCGCCGGAAACAAAATAATACTGAAAGCGAAGATCGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtgt                                                                                                                 <  1:448941/151‑1 (MQ=255)
                       ccAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtg                                                                                                                >  1:624867/1‑151 (MQ=255)
                        cAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtgt                                                                                                               >  1:115009/1‑151 (MQ=255)
                        cAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtgt                                                                                                               >  1:712865/1‑151 (MQ=255)
                         aGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtgtg                                                                                                              >  2:597147/1‑151 (MQ=255)
                          ggCTGAAAAAACAGCGAAATTTTTCTCGGCCAGTCCAAACCAGCCCCCCCCCCAAACCATGCCGGGGTGGTCGCGGGGAACAAAATAATACTGGAAGCGAAGATGGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGc                                                                                                             <  2:787108/151‑1 (MQ=255)
                                aaaTACAGCAAAATGGGTGGCGGCCAGTCCCAACCAGTCCCCCCCCGGACCCAGGGCGGGGTGGTCGCGGGGAACAGAATAATAGGGGAGGGGAAGATGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                         <  2:194325/149‑1 (MQ=255)
                                 aaTACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgc                                                                                                      >  2:359180/1‑151 (MQ=255)
                                                 tgtCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgacg                                                                                       >  1:700181/1‑150 (MQ=255)
                                                       ccgttcccACCCAGACCCCCCCCCGAACCATGACGGGAGGGCCGCGGGAAACAAAATAATACGGGAAGCAAAGATGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgacg                                                                                       <  1:834423/138‑1 (MQ=255)
                                                       ccAGTCCCAACGACTCCCCCCCCAGAACCATAGCGCGATGGTCGCCGGAAACAAAATAATACTGGAAGCGAAGTTGGCGGGTTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                  <  2:596769/149‑1 (MQ=255)
                                                            tCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACg                                                                           >  1:554470/1‑151 (MQ=255)
                                                             ccAACCAGTCCCCCCCCCGACCCATGACGGGATGGTCGCGGGAAACAAAATAATACTGGAAGGGAAGTTTGCGGGATTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTg                                                                              <  1:790063/147‑1 (MQ=255)
                                                               acccAGTCCCCCCCCCGACCCATGACGCGATGGCCGCCGGGAACAAAATAATACTGGAAGCAAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACg                                                                           <  1:143176/146‑1 (MQ=255)
                                                               aaCCAGTCCCCCCCCCGAACCAGGACGGGATGGCCGCCGGGAACAAAATAATACGGGAAGCGAAGATTGCCGGTTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGtt                                                                         <  2:194600/150‑1 (MQ=255)
                                                                  cactcccccccccGAACCATGACGCGATGGCCGCGGAAAAAAAAATAATAGTGAACGAAAAGTTTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                        <  1:187397/140‑1 (MQ=255)
                                                                       ccccccccGAACCATGACGGGATGGTCGCGGGAAACAAAATAATACTGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgcg                                                                    <  1:403515/144‑1 (MQ=255)
                                                                         gCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                                                              >  2:765204/1‑151 (MQ=255)
                                                                           ccccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAc                                                            >  1:722326/1‑151 (MQ=255)
                                                                                           gATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTg                                             >  2:341411/1‑150 (MQ=255)
                                                                                           gATGGCCGCGGGAAAGAAAAAAATCCTGGAGGCGAAGGTGCCGGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGa                                            <  1:283788/151‑1 (MQ=255)
                                                                                              ggccgcgGGGAACAAAAAAATACTGGAAGCAAAGTTTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAAt                                                      <  2:755208/135‑1 (MQ=255)
                                                                                               gTCGCCGGAAACAAAATAATCTTGGAAGGAAAGTTTGCGGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGAc                                           <  1:785550/148‑1 (MQ=255)
                                                                                                 cGCCGGGAACAGAATACTACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCCCTGTGCAGCCTAGCCACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACACTGCGGCGTTGACCAc                                        >  2:186933/1‑149 (MQ=255)
                                                                                                     ggaaaCAAAATAATACTGGAAGCGAAGTTTGCCGGTTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTc                                    <  2:154408/146‑1 (MQ=255)
                                                                                                           aaaataatACGGGAAGCGACTTTTGGCGGAATTCCCGCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                             <  1:187560/148‑1 (MQ=255)
                                                                                                           aaaaaaatccgggAAGCGAATATGGCCGGTTTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                             <  2:731199/139‑1 (MQ=255)
                                                                                                               taataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                        >  1:166405/1‑151 (MQ=255)
                                                                                                                            gAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt           >  1:453205/1‑151 (MQ=255)
                                                                                                                               gATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTg          >  2:365392/1‑149 (MQ=255)
                                                                                                                                   gcgggtaTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaaa      <  2:472793/143‑1 (MQ=255)
                                                                                                                                    cggggATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaaa      <  1:401471/146‑1 (MQ=255)
                                                                                                                                     cGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact  >  2:310720/1‑151 (MQ=255)
                                                                                                                                     cGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact  >  1:621525/1‑151 (MQ=255)
                                                                                                                                            |                                                                                                                                               
AGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACT  >  NC_000913/3443137‑3443420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑