| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,724,971 | A→C | F259C (TTC→TGC) | kgtP ← | alpha‑ketoglutarate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,724,971 | 0 | A | C | 98.2% | 214.6 / ‑3.5 | 54 | F259C (TTC→TGC) | kgtP | alpha‑ketoglutarate transporter |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (33/20); total (33/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01 | |||||||||||
CGACCAATCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCA > NC_000913/2724824‑2725110 | cGACCAATCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAAc > 2:27960/1‑151 (MQ=255) gACCAAACTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATACCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGcaa < 2:532632/149‑1 (MQ=255) ccAATCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGcaacaa > 2:755444/1‑150 (MQ=255) aaTCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAgg > 1:645491/1‑151 (MQ=255) tATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAggg < 2:505301/147‑1 (MQ=255) tATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGc < 2:689269/150‑1 (MQ=255) aTCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGccc > 2:217094/1‑151 (MQ=255) gACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGc > 2:82499/1‑149 (MQ=255) cAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCg < 1:265098/151‑1 (MQ=255) aGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAACCCGCAGCgg > 2:625561/1‑151 (MQ=255) aGCGCGCCAATGAGTGGTTGA‑‑AAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGt > 1:275569/1‑150 (MQ=255) gcgcCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTa > 1:30340/1‑150 (MQ=255) gcgcCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTa > 1:111855/1‑150 (MQ=255) cgcCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCGACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTa < 2:791180/149‑1 (MQ=255) cgcCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGCTACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTaa > 2:186580/1‑150 (MQ=255) cAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAAcc > 1:498835/1‑151 (MQ=255) aaTGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCg > 2:415109/1‑151 (MQ=255) tGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCg > 1:794495/1‑149 (MQ=255) gAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCg > 2:544643/1‑148 (MQ=255) tAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCAtgatga > 2:318501/1‑150 (MQ=255) aaGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGaa < 1:487971/150‑1 (MQ=255) tGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACg > 2:575821/1‑151 (MQ=255) gAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACg < 1:190607/150‑1 (MQ=255) aTGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCa < 1:302913/151‑1 (MQ=255) cAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATcc > 1:8657/1‑151 (MQ=255) tCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCttt > 1:684809/1‑151 (MQ=255) tCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCttt > 1:637745/1‑151 (MQ=255) tAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTc > 1:201406/1‑149 (MQ=255) aaTGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCaga < 2:145842/151‑1 (MQ=255) gCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCag > 1:342242/1‑147 (MQ=255) gCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAgaga < 1:740190/150‑1 (MQ=255) cACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATcc < 2:506774/151‑1 (MQ=255) cGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAg < 2:339811/149‑1 (MQ=255) gCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGctt < 1:582078/151‑1 (MQ=255) aCGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGcttctt < 2:279006/151‑1 (MQ=255) ggCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTaaa < 2:275520/151‑1 (MQ=255) atgcatTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAgcg > 1:509239/1‑151 (MQ=255) tgcatTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAgcgc > 2:359832/1‑151 (MQ=255) gcatTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAgcgc < 2:313586/150‑1 (MQ=255) catTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAgcgcgc < 1:475527/151‑1 (MQ=255) atTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGcgcgcg > 1:343504/1‑151 (MQ=255) ccGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTc < 2:50352/151‑1 (MQ=255) cGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCt < 2:408439/151‑1 (MQ=255) aGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCttgt > 2:396133/1‑151 (MQ=255) aGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCttgt < 2:16091/151‑1 (MQ=255) gATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTc > 2:330856/1‑151 (MQ=255) gATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTc > 1:252951/1‑151 (MQ=255) gATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTc > 2:335229/1‑151 (MQ=255) aTACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTc > 1:352563/1‑150 (MQ=255) cTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCt > 1:296943/1‑151 (MQ=255) gCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTg > 1:802532/1‑151 (MQ=255) cATATAGGTAGTGAAGGTATAGCAACCTAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGAACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTg > 2:165379/1‑150 (MQ=255) atatAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGAGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTgac > 2:453246/1‑151 (MQ=255) tataAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTgacg > 1:238243/1‑151 (MQ=255) agtagtGAAGGTATAGCAACAAAGGGAGCCCGCAGTGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGt < 1:532636/151‑1 (MQ=255) gAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACca < 2:573144/150‑1 (MQ=255) | CGACCAATCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCA > NC_000913/2724824‑2725110 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |