Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,984,441 | 0 | A | C | 46.4% | 99.4 / 10.9 | 57 | G384G (GGT→GGG) | yqeF | short chain acyltransferase |
Reads supporting (aligned to +/- strand): ref base A (29/1); new base C (0/26); total (29/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.06e-15 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGC > NC_000913/2984292‑2984591 | gAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑acac > 1:919654/1‑151 (MQ=255) gAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTAAGGAGAAAAGGTCAATGCC‑acac > 1:387278/1‑151 (MQ=255) atatGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACAccc < 2:803038/151‑1 (MQ=255) tGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGccccc > 1:975110/1‑151 (MQ=255) aaTGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTTCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCccgccg > 2:36630/1‑151 (MQ=255) aaTGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCccgccg > 1:123575/1‑151 (MQ=255) aaaaTGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACa > 2:368200/1‑150 (MQ=255) aaaaTGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACa > 1:41763/1‑150 (MQ=255) gAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGt > 2:211977/1‑151 (MQ=255) gggAGCCCAAAAAATGTATGCCAACTCGCCGCCCATCATTATCAAACAGATAAGTGCCGCAAGATATTGATCGGAAAGGAGAAAATGCACAGCTATTCGTCTCGTTCAATGGACAAGGCC‑CCCCCCTGGCCCCCGCCGATACAAAGCGTTg < 1:992674/151‑1 (MQ=255) ccAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTc > 1:303193/1‑151 (MQ=255) aaaaaTGTTGGTCGACTGGCCACCAATAATAATCAACCAGATTAATGCCGCCAAATTTTTACCGGAAAGGAGAAAAGGCAGACTTTTTCGTCACTTTCAAGGGTCAAGCCC‑ACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCtt < 1:605990/150‑1 (MQ=255) aaaaTGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCttt > 2:84179/1‑151 (MQ=255) tatgtCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGc > 2:594751/1‑151 (MQ=255) gtcgtcTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAGGGAGAAAATGCAGAGCTTTTCGTCACGTTCAATGGTCAATCCC‑CCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGc < 1:377376/148‑1 (MQ=255) gtCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCAtt > 1:613722/1‑151 (MQ=255) cgccTTGCCAGCCATCATTATTCAACAAATTAATGACGGAAGATATTTATCGGTAAGGAGAAAATGCAGAGCTATTCGTCACGTTAAATGGTCAATGCC‑ACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTAc < 2:779169/148‑1 (MQ=255) gACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGC‑‑‑CACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGtt > 2:429027/1‑151 (MQ=255) tggccagccaTCATTATTAAACAGATAAAGGCCGGCAGATATTTATCGGAAAGGAGAAAATGCAGAGTTTTTCGTCACGTTAAAGGGTCAGTCCC‑ACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACg < 1:965177/146‑1 (MQ=255) gccagccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTc > 1:377209/1‑150 (MQ=255) gccagccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTc > 1:881290/1‑150 (MQ=255) gccagccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTc > 2:954922/1‑150 (MQ=255) atttttataCAACAGATTAAGAACGGCAGATATTTACGGGAAGGGAGAAAATGCAGATCTTTTCGTCACTTTAAAGGGTCAATCCC‑CCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCAt < 1:462001/144‑1 (MQ=255) atcatTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTc > 2:747980/1‑151 (MQ=255) ttattaAACAGATTAATGACGGCAGATTTTTAGCGGAAAGGAGGAAATGCAGAGCTTTTCGCCACGTTCAATGGTCAATCCC‑CCCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATg < 1:223445/150‑1 (MQ=255) ttattCAACAGATAAATGACGGCAAATATTTATCGGAAAGGAGAAAATGCAGCCCTTTTCGTCACGTTCAATGGTCAATGCC‑CCCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTc < 1:130687/147‑1 (MQ=255) ttataCAACAGATTAAGGACGGCAGATATTTATCGGTAAGGAGGAAATGAAGAGTTATTCTTCACGTTCAATGGTCAATGCC‑CCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCAttt < 1:134765/146‑1 (MQ=255) tattAAACAGATAAATGACGGCAGATATTTATCGGAAAGGAGAAAAGGCAGAGCTTTTCGTCCCGTTAAATGGTAAATGCC‑ACCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGa < 2:819085/150‑1 (MQ=255) attCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAAc > 2:577669/1‑151 (MQ=255) attAAACAGATTAATGACGGCAGATATTTATCGGAAAGGAGGAAATGAAGAGCTTTTCGTCACGTTAAATGGTCAATGCC‑CCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGa < 2:350498/149‑1 (MQ=255) atcaaaCAGATTAATGACGGCAGATTTTTAGGGGTAAGGAGAAAATGAAGAGTTTTTCGTCACGTTAAAGGTTCAAGCCC‑ACCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGaa < 2:169166/146‑1 (MQ=255) ttCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAAcc > 2:434561/1‑151 (MQ=255) tCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAAcc > 2:420640/1‑150 (MQ=255) aaCAAATTAAGGACGGCAGATATTTATCGGAAAGGAGAAAATGCAGACTTATTCGCCAGTTTCATTTGTCAATCCC‑ACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCaga < 1:268558/151‑1 (MQ=255) aGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAgaga > 2:333131/1‑150 (MQ=255) aGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAgaga > 2:898433/1‑150 (MQ=255) aTTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAAc > 1:962076/1‑151 (MQ=255) aaggagggCAGATATTTATCGGTAAGGAGAAAATGCAGAGTTTTTCGTCACGTTAAAGGGTCAATGCC‑CCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCa < 2:380577/144‑1 (MQ=255) aTGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAgg > 2:752710/1‑151 (MQ=255) cgccaaatatTTATCGATAAAGACAAAATCCAGATCTATTCTCCATTTTCATTTTTCAATCCC‑CCCCCCTCCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCTGTCATTACGTTTCACCATTTCATGAACCACATAAACCATCATTc < 1:86544/145‑1 (MQ=255) gcgaGATATTTTTCGGTAAGGAGGAAGCGTAGAATTTTTCGTCAC‑CTAAAACGCGAATGCCAACGCCCCGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGAtt < 2:788331/146‑1 (MQ=255) gCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCgg > 2:43089/1‑151 (MQ=255) tatTTATCGGTAAGGAGGAAATGCAGAGTTTTTCGTCACGTTAAATGGTAAATGCC‑CCCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCa < 2:882782/148‑1 (MQ=255) attgaccGGTAAGGAGAAAAGGCAGAGTTATTCGCCACGTACAATGGTCAGGCCC‑CCCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGAt < 1:350147/135‑1 (MQ=255) tttATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCgg > 2:583234/1‑151 (MQ=255) cggaagggagaaAATGCAGAGTTATTCGTCACGTTCAATGGTCAATCCC‑ACCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAgc < 1:63735/144‑1 (MQ=255) ggTAAGGAGGAAATGCAGAGCTTTTCGTCCCTTTAAATGGTCAATGCC‑CCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAgcg < 2:3677/150‑1 (MQ=255) gTAAGGAGAAAATGCAGAGCTTTTCGTCACGTTCAATGGTCAATCCC‑ACCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAg < 2:21079/147‑1 (MQ=255) ggagaaAATGCAGAGCTTTTCGTCACTTTAAATGGCAAAGCCC‑ACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTAt < 2:805317/150‑1 (MQ=255) aggaAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCgg > 1:947620/1‑151 (MQ=255) aaaTGCAGAGCTTTTCGTCACGTTAAATGGTAAATCCC‑ACCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGt < 2:919790/150‑1 (MQ=255) tATTCGCCACTTTAAATGGTAAACGCC‑CCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAg < 1:656186/146‑1 (MQ=255) gTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATcgcg > 2:771803/1‑151 (MQ=255) tCACGTTCAATGGTCAATGCC‑ACACCATGGCCCCCGCCGATACAAAGCGTAGCCAGTCCTTTGCGGGCATTACGCTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGCGTGCGATcgacc > 1:148434/1‑148 (MQ=255) cACGGTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACAAGAGAAACCAGGCTTCGGCAACCGGAATCGCCTATCGGGTGACCGAGTGCGATCGCGcc > 1:895159/1‑151 (MQ=255) ttCAATGGTAAATGCA‑ACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGcca < 2:624167/148‑1 (MQ=255) aaTGGTCAATCCC‑ACCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattg < 1:84173/151‑1 (MQ=255) gtaaaTCCC‑ACCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattgacat < 2:561074/148‑1 (MQ=255) gTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattgacat > 2:894811/1‑151 (MQ=255) c‑acaCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCg > 1:672260/1‑150 (MQ=255) acCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGc > 2:988104/1‑151 (MQ=255) | GAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCC‑ACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGC > NC_000913/2984292‑2984591 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |