Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,724,971	A→C	F259C (TTC→TGC)	kgtP ←	alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,724,971	0	A	C	100.0%	238.6 / NA	58	F259C (TTC→TGC)	kgtP	alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (26/32);  total (26/32)

GACCAATCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGC  >  NC_000913/2724825‑2725116                                                                                                                                                   |                                                                                                                                                  gACCAATCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAAc                                                                                                                                                >  1:226736/1‑150 (MQ=255) gACCAATCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAAc                                                                                                                                                <  1:31688/150‑1 (MQ=255) gACCAATCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAAc                                                                                                                                                >  1:475496/1‑150 (MQ=255)     aaTCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAgg                                                                                                                                           >  1:100601/1‑151 (MQ=255)          tATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGa                                                                                                                                         <  1:425349/148‑1 (MQ=255)           aTCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGccc                                                                                                                                     <  2:462675/151‑1 (MQ=255)           aTCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGccc                                                                                                                                     <  1:679316/151‑1 (MQ=255)              cGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCa                                                                                                                                  <  2:932524/151‑1 (MQ=255)               gACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGc                                                                                                                                   >  2:360897/1‑149 (MQ=255)               gACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGc                                                                                                                                   >  2:144195/1‑149 (MQ=255)                  aGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCgg                                                                                                                              <  2:805576/151‑1 (MQ=255)                    cgcgcCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTa                                                                                                                            <  1:77056/151‑1 (MQ=255)                     gcgcCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTa                                                                                                                            >  1:603488/1‑150 (MQ=255)                        ccAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAAc                                                                                                                        <  2:838334/151‑1 (MQ=255)                          aaTGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCg                                                                                                                      <  2:65006/151‑1 (MQ=255)                            tGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCg                                                                                                                      >  2:949954/1‑149 (MQ=255)                             gAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCgag                                                                                                                    <  2:573158/150‑1 (MQ=255)                                tGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAAcc                                                                                                                >  1:501181/1‑151 (MQ=255)                                 ggTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAAcc                                                                                                                >  2:940165/1‑150 (MQ=255)                                  gTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCat                                                                                                              <  1:636526/151‑1 (MQ=255)                                       aTAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCatgatg                                                                                                          >  2:241872/1‑150 (MQ=255)                                               gAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACgg                                                                                                 >  1:162156/1‑151 (MQ=255)                                                aaTACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGc                                                                                                <  1:224083/151‑1 (MQ=255)                                                 aTACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCg                                                                                               <  2:816751/151‑1 (MQ=255)                                                   acaaacaaTGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGAt                                                                                             <  1:309732/151‑1 (MQ=255)                                                       acaaTGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTgcg                                                                                         >  1:613874/1‑151 (MQ=255)                                                        caaTGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTgcgc                                                                                        >  1:508590/1‑151 (MQ=255)                                                                 aGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCt                                                                               >  1:736833/1‑151 (MQ=255)                                                                 aGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCt                                                                               <  1:205653/151‑1 (MQ=255)                                                                    cATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTc                                                                            >  1:762645/1‑151 (MQ=255)                                                                     aTAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCa                                                                           <  1:503097/151‑1 (MQ=255)                                                                        aTGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCagag                                                                        <  2:740140/151‑1 (MQ=255)                                                                          gCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAgaga                                                                       <  2:239668/150‑1 (MQ=255)                                                                            cACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATcc                                                                    <  2:418896/151‑1 (MQ=255)                                                                                         ggCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTaaa                                                       <  2:547946/151‑1 (MQ=255)                                                                                         ggCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTaa                                                        <  1:987114/150‑1 (MQ=255)                                                                                         ggCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTCCAGAGATCCAGCTTCTTTTaaa                                                       <  1:645120/151‑1 (MQ=255)                                                                                            atgcatTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAgcg                                                    <  2:567060/151‑1 (MQ=255)                                                                                              gcatTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAgcgcg                                                  <  2:244449/151‑1 (MQ=255)                                                                                              gcatTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAgcgcg                                                  >  2:739728/1‑151 (MQ=255)                                                                                                   cccGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGttt                                             <  2:436607/151‑1 (MQ=255)                                                                                                       cAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCt                                           >  2:671171/1‑149 (MQ=255)                                                                                                               aCCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGaa                                  <  1:1002051/150‑1 (MQ=255)                                                                                                                        ttCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCt                         >  1:538104/1‑150 (MQ=255)                                                                                                                         tCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTa                        >  2:66218/1‑150 (MQ=255)                                                                                                                         tCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTa                        >  2:34786/1‑150 (MQ=255)                                                                                                                            gCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAAc                      <  2:262751/149‑1 (MQ=255)                                                                                                                            gCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACt                     <  1:458324/150‑1 (MQ=255)                                                                                                                               tataAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTgacg                 >  2:939539/1‑151 (MQ=255)                                                                                                                                ataAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTgacga                <  2:788375/151‑1 (MQ=255)                                                                                                                                 taAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTgacgac               >  2:661255/1‑151 (MQ=255)                                                                                                                                         gAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACcac       >  2:18942/1‑151 (MQ=255)                                                                                                                                          aaGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACcac       >  2:361423/1‑150 (MQ=255)                                                                                                                                          aaGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACcac       >  1:48419/1‑150 (MQ=255)                                                                                                                                           aGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACaa     <  1:290487/151‑1 (MQ=255)                                                                                                                                            ggTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGAGAAGTTTCATCTAACTGACGACGTAACCACAAc    <  2:418166/151‑1 (MQ=255)                                                                                                                                               ataGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGc  <  2:269433/150‑1 (MQ=255)                                                                                                                                               ataGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGc  >  1:924645/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GACCAATCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGC  >  NC_000913/2724825‑2725116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑