| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,969,051 | +G | coding (21/1047 nt) | wzzE → | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,969,048 | 1 | . | G | 88.9% | 192.0 / 14.5 | 54 | coding (18/1047 nt) | wzzE | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Reads supporting (aligned to +/- strand): ref base . (4/2); new base G (26/22); total (30/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
TGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCT‑G‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCGTATACGTTTTTTGCTCGTC > NC_000913/3968898‑3969187 | tGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCt > 1:531701/1‑151 (MQ=255)tGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCt > 2:557607/1‑151 (MQ=255) cTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCACTTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCT‑g‑g > 1:566323/1‑151 (MQ=255) tAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCT‑g > 1:540797/1‑149 (MQ=255) gCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACTCAGACTGCGTAGAAATCGTGGTGGCAGCCCAAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCT‑g‑ggg < 1:478330/150‑2 (MQ=255) cttcttcCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCT‑T‑GG‑gaa > 1:144085/2‑150 (MQ=255) aTTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCT‑G‑GG‑gaaa < 2:606823/151‑2 (MQ=255) aTTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCT‑G‑GG‑gaa > 1:106087/1‑150 (MQ=255) tcttcCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAAcc > 1:556194/1‑151 (MQ=255) cttcCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCg > 2:557656/1‑151 (MQ=255) ttcCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCgg > 1:342706/1‑151 (MQ=255) tcCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCg < 2:545996/149‑1 (MQ=255) aTGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGaa < 2:303888/149‑1 (MQ=255) ggATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGAc < 1:141663/150‑1 (MQ=255) ggATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACg > 2:533142/1‑151 (MQ=255) ggATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACg < 1:314750/151‑1 (MQ=255) aaGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAAc > 1:41169/1‑151 (MQ=255) aaGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAAc > 2:435155/1‑151 (MQ=255) gTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTgg > 2:385344/1‑150 (MQ=255) ccTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGatat < 2:47857/151‑1 (MQ=255) tGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTc > 2:44778/1‑151 (MQ=255) gAAAGCTGCTCGCTTTATTCAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCg > 1:618963/1‑150 (MQ=255) cGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGgttgtt < 2:348094/149‑1 (MQ=255) cGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTc < 2:40774/151‑1 (MQ=255) ttattaAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTAc > 2:238118/1‑151 (MQ=255) tattaAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTAcc > 2:619276/1‑151 (MQ=255) tattaAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTAcc > 1:112795/1‑151 (MQ=255) tattaAGCGAGTAAAACGCAGACTGCGTAGAAACCGTGTTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACGCAACCAATGCCT‑GAGG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGCTATTCGTGGGTTGTTTCGTAcc > 1:153590/1‑151 (MQ=255) attaAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCt > 1:274520/1‑151 (MQ=255) aaGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTtg < 1:77821/150‑1 (MQ=255) aaGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTtg < 2:339702/150‑1 (MQ=255) aGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTtgtg < 2:84940/151‑1 (MQ=255) cgTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCt < 2:598526/151‑1 (MQ=255) tAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTgg > 1:93518/1‑151 (MQ=255) aaCGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGaa < 1:337413/151‑1 (MQ=255) gCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCt > 1:116936/1‑151 (MQ=255) gCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCt < 1:98872/151‑1 (MQ=255) tGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGat > 2:510749/1‑151 (MQ=255) gCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGatt > 2:594501/1‑151 (MQ=255) gTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGattat > 2:474662/1‑151 (MQ=255) aGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTg < 1:585330/151‑1 (MQ=255) aaTCGTGGTGGCAGCCCCAACTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCa < 1:607264/151‑1 (MQ=255) gtggtgGCAGCCCCAATTTAACCAAATAGATGAGGGTGTGATGACAGAACCAATGACT‑G‑GGCAAACCGGCCGAAGATGCTGAAACATAACTGGATATTCGTGGGGTGACTCGTACATCGCGGGCTGGGACGCTATGGCTTATTGGCAt > 1:667933/1‑148 (MQ=255) gtggCAGCCCCAATTTAACCAAATAAATGAAGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCt < 2:336704/151‑1 (MQ=255) gCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGtt > 2:420347/1‑151 (MQ=255) aaCCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATc < 1:625608/151‑1 (MQ=255) aaCCAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATc > 2:658725/1‑151 (MQ=255) ccAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATcgc < 1:452811/151‑1 (MQ=255) ccAAATAAATGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATcgc < 2:595996/151‑1 (MQ=255) aaataaatGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCgcgc > 2:417259/1‑151 (MQ=255) ataaatGAGGATGTGATGACACAACCAATGGCT‑GTGG‑AAACCGGCAGAAGACGCTGAAAATGAACTGGATAGGCGTGGGTTGTTTCGTGCCTTGTGTGCTGGGAAGCTATGGATTATGGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTg < 1:35401/151‑1 (MQ=255) aaatGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTTGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGc < 1:460275/151‑1 (MQ=255) aaatGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGc > 2:493928/1‑151 (MQ=255) atGAGGATGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGACGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCg > 1:412370/1‑150 (MQ=255) aTGTGATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCGTATACGt > 2:2530/1‑151 (MQ=255) gtgATGACACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCGTATACGttt > 2:512830/1‑151 (MQ=255) atgaCACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCGTATACGtttttt < 2:634012/151‑1 (MQ=255) gaCACAACCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCGTATACGTTTTTTg < 1:578416/150‑1 (MQ=255) aaCCAATGCCTGG‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCGTATAAGTTTTTTGCTCGTc > 1:363716/1‑151 (MQ=255) | TGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCT‑G‑GG‑AAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACCTTGTGGGCTGGGAAGCTATGGATTATTGGCATGGGGCTGGCGTTTGCGTTAATCGCGCTGGCGTATACGTTTTTTGCTCGTC > NC_000913/3968898‑3969187 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |