Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,725,518	+C	coding (229/1299 nt)	kgtP ←	alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,725,518	1	.	C	100.0%	187.7 / NA	51	P77A (CCA→GCA)	kgtP	alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (26/25);  total (26/25)

ATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGG‑GCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTG  >  NC_000913/2725368‑2725661                                                                                                                                                        |                                                                                                                                                ataatGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTgg                                                                                                                                                  >  2:206742/1‑151 (MQ=255)  taatGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCAGGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTgg                                                                                                                                                  <  1:555192/150‑1 (MQ=255)                  gTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATccc                                                                                                                                 >  2:341289/1‑151 (MQ=255)                   tACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCg                                                                                                                                >  2:305564/1‑151 (MQ=255)                     ccTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACCTCAATCACCCCTATAACAGCATCGTTTTTTTTCGACCATGTTTATCGTTTATGCGGCCAAATAGCCAACCGCCTCTTGGCGCGCATCAGGAATccc                                                                                                                                 <  1:503875/148‑1 (MQ=255)                          aGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCaaa                                                                                                                         <  2:642546/151‑1 (MQ=255)                           gTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCaaaa                                                                                                                        <  1:242066/151‑1 (MQ=255)                                     cTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAACCACctgctg                                                                                                              >  1:366131/1‑151 (MQ=255)                                       gggAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACctgctg                                                                                                              <  2:511626/149‑1 (MQ=255)                                          aggcaggcGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTgt                                                                                                         >  1:413506/1‑151 (MQ=255)                                             cgggcGATACCCACCGATCCGAAACACAACACACCCACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTgta                                                                                                        <  2:205142/147‑1 (MQ=255)                                             caggcGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTgtagt                                                                                                      >  1:683370/1‑151 (MQ=255)                                                 cGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGtt                                                                                                  >  1:177566/1‑151 (MQ=255)                                                  gATAACCAGCGGTCCGAAACACATCATACACACCGATAACAGCATCGCTTTTTTGCTACCATGTTTATCGGCTAGGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACGCCTGCTGTTTGTAGTAGTTg                                                                                                 <  1:170362/151‑1 (MQ=255)                                                      aCCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAgtc                                                                                             <  1:139799/151‑1 (MQ=255)                                                       ccAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAgtcg                                                                                            >  2:301903/1‑151 (MQ=255)                                                       ccAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAgtc                                                                                             <  2:377907/150‑1 (MQ=255)                                                          gCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGtcgtcg                                                                                         <  2:62506/151‑1 (MQ=255)                                                           ctaTCCAAAACACATCATACACACCTATAACATCATCTATTTTTTCCTACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGtcgtcg                                                                                         <  2:350382/148‑1 (MQ=255)                                                            gATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCgtg                                                                                       >  1:440210/1‑151 (MQ=255)                                                                cGAAACACATCATACACCCCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACGCCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTcc                                                                                   <  1:210744/151‑1 (MQ=255)                                                                cGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTcc                                                                                   <  2:241039/151‑1 (MQ=255)                                                                      acaTCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCgga                                                                               >  1:465599/1‑149 (MQ=255)                                                                      acaTCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAAACGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCgga                                                                               >  1:451310/1‑149 (MQ=255)                                                                            tACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCggaaggga                                                                          >  1:580695/1‑148 (MQ=255)                                                                                   cGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGAtgtg                                                                <  1:206629/151‑1 (MQ=255)                                                                                     aTAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTggg                                                              >  2:209318/1‑151 (MQ=255)                                                                                            cATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTa                                                       <  2:16280/151‑1 (MQ=255)                                                                                             aTCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGt                                                        >  1:357303/1‑149 (MQ=255)                                                                                             aTCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGt                                                        >  2:48913/1‑149 (MQ=255)                                                                                             aTCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTag                                                      <  2:529688/151‑1 (MQ=255)                                                                                                 aTTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTg                                                  >  2:166220/1‑151 (MQ=255)                                                                                                       tGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAAc                                               >  2:441188/1‑148 (MQ=255)                                                                                                       tGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAg                                             <  1:455229/150‑1 (MQ=255)                                                                                                        gCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGa                                            <  2:459656/150‑1 (MQ=255)                                                                                                          gACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACg                                         <  1:360085/151‑1 (MQ=255)                                                                                                           aCCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACg                                         >  2:445349/1‑150 (MQ=255)                                                                                                              aTGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTa                                     <  1:616329/151‑1 (MQ=255)                                                                                                                     tCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGACGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACAt                                >  1:273429/1‑149 (MQ=255)                                                                                                                               ggCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAAcc                    >  1:684075/1‑151 (MQ=255)                                                                                                                               ggCCAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAAc                     <  2:331803/150‑1 (MQ=255)                                                                                                                                 ccAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAGACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCAc                  <  2:545955/151‑1 (MQ=255)                                                                                                                                  cAAATAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACt                 >  1:600565/1‑151 (MQ=255)                                                                                                                                   aaaTAGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTc                >  2:308655/1‑151 (MQ=255)                                                                                                                                       aGCCAACCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGAcc            >  2:310070/1‑151 (MQ=255)                                                                                                                                           aaCCGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGAt        <  2:376329/151‑1 (MQ=255)                                                                                                                                             ccGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGAtt       >  2:218405/1‑150 (MQ=255)                                                                                                                                             ccGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTa      <  2:169464/151‑1 (MQ=255)                                                                                                                                             ccGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTa      <  1:264169/151‑1 (MQ=255)                                                                                                                                              cGCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTAc     <  2:683889/151‑1 (MQ=255)                                                                                                                                               gCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTAcc    >  2:571393/1‑151 (MQ=255)                                                                                                                                               gCCTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTAcc    >  1:74131/1‑151 (MQ=255)                                                                                                                                                 cTATTGGCGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTg  >  2:104489/1‑151 (MQ=255)                                                                                                                                                        |                                                                                                                                                ATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGG‑GCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTG  >  NC_000913/2725368‑2725661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑