Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913300,7210GT38.2% 42.4 / 13.1 34Q72K (CAA→AAA) paoCPaoABC aldehyde oxidoreductase, Moco‑containing subunit
Reads supporting (aligned to +/- strand):  ref base G (7/14);  new base T (13/0);  total (20/14)
Fisher's exact test for biased strand distribution p-value = 1.60e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCTCGGCCACTACCAGCGCAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCAGCGGTCCGTCGATGCGGTCAT  >  NC_000913/300579‑300870
                                                                                                                                               |                                                                                                                                                       
gTCTCGGCCACTACCAGCGCAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCTGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGt                                                                                                                                                 <  1:356905/151‑1 (MQ=255)
    cGGCCACTACCAGCGCAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTcc                                                                                                                                               <  2:197126/149‑1 (MQ=255)
       ccACTACCAGCGCAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATc                                                                                                                                          <  1:444918/151‑1 (MQ=255)
         aCTACCAGCGCAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAAAGACAGCCAGTACGCCCCGCGCCTTTTTTGCGGCGGCCGGATc                                                                                                                                          >  2:499003/1‑149 (MQ=255)
              cAGCGCAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTGATTACAGCCCCGACGCCCCGCGCCTTTTTCGCGGGGGCCGGATCAAggg                                                                                                                                    >  1:257664/1‑149 (MQ=255)
               aGCGCAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGGAACCCCCGCGCCTTTTTCGCGGCGGCCGGATCCAAGGCgg                                                                                                                                 >  2:503397/1‑151 (MQ=255)
                      tGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATTAAAGCCAATACGCCCCGCGCTTTTTTTGCGGGGGCCGTATCAAGGGGGGGGAgggg                                                                                                                          >  2:566240/1‑149 (MQ=255)
                            gataatAGTGGTCAATAGAGGGGCCGCCTAACAGCATGGCGGTTTTTTTGTCGACTTTTCAGAGTGACCCTGCTATACTGTTGTTAATGACAGCCAGTACGCCCGGCGGTTTTTGCGCGGCGTCCGCATCAAGTGCGTTGAGGCGACCtt                                                                                                                     <  1:540108/150‑1 (MQ=255)
                                      cTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGTATTACAGACAAGAAGGCCGGCGGCTTTTTTTCCGGGGCCGGATTAAGGGCGGGGGGGGGGCCTTTGGcc                                                                                                                >  1:350193/1‑144 (MQ=255)
                                                gggCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTGCTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACcgacga                                                                                               <  1:100789/151‑1 (MQ=255)
                                                  gccgccTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAACCAGTACGCCCCGCGCTTTTTTCGCGGCGGCCGGATCAAAGGGGGGGGGGGGGCCCTTGGGCAAGGCGGGAAcgacga                                                                                               >  2:539591/1‑149 (MQ=255)
                                                    cgccTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATTGAAGCCCGCACGCCCCGCGGCTTTTTCGCGGCCGCCCTATCCAGGGCGGGGGGGCGGCCCTTTGGGATGGCGGGACccacgaaa                                                                                             >  1:297312/1‑147 (MQ=255)
                                                      ccTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGc                                                                                         <  1:477716/151‑1 (MQ=255)
                                                          aCAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGAATTTAAGCCAGTACCCCCCGCGCCTTTTTCGCGGCGTCCGGATCCAGGGGGGGGAGGGGGCCCTTTGCCATGGCGGGAACcgaggtataagca                                                                                        >  1:183925/1‑139 (MQ=255)
                                                           cAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAg                                                                                    <  2:634777/151‑1 (MQ=255)
                                                                 ggCGGTGTTTTTGTCTCCTGTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCGGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGgcggcg                                                                              <  2:243424/151‑1 (MQ=255)
                                                                 ggCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAggcggc                                                                               <  2:309933/150‑1 (MQ=255)
                                                                   cGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAACCAGTACGCCCCGCGCTTTTTGCGCGGCGGCCGGATCAAGGGCGGGGAGGCGGCCTTTGGCCATGGCGGGAACGAAGATATAGCCAAAGgcggcggt                                                                            >  2:80299/1‑149 (MQ=255)
                                                                      tgtTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATTACAGCCCGTACGCCCCGCGCCTTTTTCGCGGCGTCCCTATCAAGGGGGGGGGGGCGTCCCTTGGCAATGGCGGGACCGGCGATATAGCCACAGGCGGCGGTggg                                                                         >  1:533868/1‑151 (MQ=255)
                                                                        tttttGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTgggg                                                                        <  2:334984/150‑1 (MQ=255)
                                                                        tttttGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCCGCGCTTTTTTCGCGGCGGCCGGATCAAGGGGGGGGAGGCGGCCCTTGGCAATGGCGGGAACGGCGAGAAAGCCAAAGGGGGGGGTggggg                                                                       >  1:679108/1‑151 (MQ=255)
                                                                        tttttGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGGAAGCCCCGCGCTTTTTTCGCGGGGGCCGGATCAAGGGGGGGGAGGGGTCCTTTTGCAATGGGGGGACCGACGAAATAGCCATAGGCGGCGGTggggg                                                                       >  2:562726/1‑151 (MQ=255)
                                                                                        cGAGTGCCCCGGCGTTACTGGCGGTAAAGACAGCCCGTACGCCCCGCGCCTTTTTCGCGGCGTCCGCATCCAGGGCGGGGAGGCGGCCTTTGGCAATGGCGGGACCGCCCATATAGCCATAGGCGGCGGTTGGGGGGTCTTCATGCCCtt                                                        >  1:158138/1‑150 (MQ=255)
                                                                                                  ggCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAg                                             <  1:262112/151‑1 (MQ=255)
                                                                                                   gCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGc                                            <  1:322670/151‑1 (MQ=255)
                                                                                                              ggcaaTGACAGCCCGTACCCGCGGCGCTTTTTTCGCGGCGTCCGTCTCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGCACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCCTGCCATTCGTAGGCGt                                               >  1:205106/4‑137 (MQ=255)
                                                                                                               gTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCgg                                >  2:223895/1‑151 (MQ=255)
                                                                                                                 aaTGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTa                              <  2:326457/151‑1 (MQ=255)
                                                                                                                           aGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCAGCg                    >  1:286036/1‑151 (MQ=255)
                                                                                                                             tACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCAGCGGt                  >  2:447586/1‑151 (MQ=255)
                                                                                                                                gCCCGGCGCTTTTTGCGCGGGGGCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCAGCGGTct                <  1:366969/150‑2 (MQ=255)
                                                                                                                                        gcTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCAGCGGTCCGTCGATGc        >  2:331762/1‑151 (MQ=255)
                                                                                                                                           ttttGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCAGCGGTCCGTCGATGCGGt     >  2:518862/1‑151 (MQ=255)
                                                                                                                                             ttGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCAGCGGTCCGTCGATGCGGTc    >  1:564325/1‑150 (MQ=255)
                                                                                                                                               gcgcGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCAGCGGTCCGTCGATGCGGTCag  <  1:472050/150‑2 (MQ=255)
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GTCTCGGCCACTACCAGCGCAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCAGCGGTCCGTCGATGCGGTCAT  >  NC_000913/300579‑300870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: