Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913357,8730CT37.9% 26.5 / 11.3 30S273N (AGC→AAC) cynRtranscriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (7/11);  new base T (0/11);  total (8/22)
Fisher's exact test for biased strand distribution p-value = 2.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCG  >  NC_000913/357724‑358014
                                                                                                                                                       |                                                                                                                                             
cACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACTGTGATTCATTCCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCt                                                                                                                                              <  1:258469/151‑1 (MQ=255)
    gAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAt                                                                                                                                            <  2:393915/149‑1 (MQ=255)
    gAACTCGGAGGCCTGCTAAGCGTAGCGCATCAGCCCATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTa                                                                                                                                             <  1:282063/148‑1 (MQ=255)
         cGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTc                                                                                                                                       <  1:309652/149‑1 (MQ=255)
             ggCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCcgccgc                                                                                                                                 >  2:391089/1‑151 (MQ=255)
                     aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAc                                                                                                                         <  1:661220/151‑1 (MQ=255)
                        cttcgcacATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAcc                                                                                                                        <  1:40172/142‑1 (MQ=255)
                        cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgc                                                                                                                      >  1:75540/1‑151 (MQ=255)
                                       actcccccccGCAGACTTCGGTCAGCGCCTCCCGGGTTTCTTTACCCCACACACCCGCGTTTTTTCCAACCCCCTGGGAAAAAAATCCCTTCGGGGGGGTTGCCTCCCAGTTTTTTTTCCGCGCAAACAAAACCGCCGTTCTCTCCagt                                                                                                         <  1:197917/141‑1 (MQ=255)
                                       aaTCCCAGCCGCAGCCCTGTGTCAGCGGCTACCGTGTTTCTTTCCCGCCACCACCCGCGCTTTTACCCAACGCCATTGGCAAAAATCCCTCCGGGGCGGCTGTCTCCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCagta                                                                                                        <  2:561137/150‑1 (MQ=255)
                                         ttCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTTATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGATATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTg                                                                                                     >  1:617442/1‑151 (MQ=255)
                                                  ctgacttgtacaaGCGGCTACCGGGATCCATTCCCGCCAACACCCGCGCTTTTATCCAACGCCATGTAAAAAACTGCCTGCGCGGCGGCTGTCTGCGAGCTATTGTGCCGCCGCAACAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCa                                                                                             <  1:147332/138‑1 (MQ=255)
                                                               gCGGCTACCGTGATTCATTCCCCCCAACAACCGCGCTTTTACCCACCGCCATGTCAAAAAATGCCTCCGGGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCttt                                                                               <  2:124995/151‑1 (MQ=255)
                                                                 ggCTCCCGTGTTTCTTTCCCGCCAACACCCGCCCTTTTACCCAACCCCATGTAAAAAAAGCCTTGCGCGGGGCCTGTCTCCCAGTTTTTTTTCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTa                                                                              <  2:9295/150‑1 (MQ=255)
                                                                 ggCTCCCGTGTTTCTTTCCCCCAAACACCCGCGCATTTACCCACCCCCTTGTGAAAAAATGCCTTCGCGGCGGCTTTCTCCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTaa                                                                             <  2:557842/151‑1 (MQ=255)
                                                                   cTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGaaaaaaattttaa                                                                            >  2:28866/1‑140 (MQ=255)
                                                                      ccgtatttCATTCCCCCCAACACCCCCGCTTTTACCCACCCCCATGTGAAAAAATGCTTTCGCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGcc                                                                          <  2:298204/143‑1 (MQ=255)
                                                                            ttCTTTCCCGCCAACACCCGCGCTTTTACCCACCCCCATGTCAAAAAATCCCTCCGCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTc                                                                      <  2:312514/147‑1 (MQ=255)
                                                                            ttCATTCCCCCCAACACCCGCGCTTTTACCCACCGCCATGTGAAAAAATGCCTCCGGGGCGGCTGTCTGCCAGTTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCAtg                                                                   <  2:152515/150‑1 (MQ=255)
                                                                               aTTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGAGAGAAAAAACATTTAACCCGTCCtgttgt                                                               >  2:47267/1‑151 (MQ=37)
                                                                                 tcccGCCACCACCCGCGCATTTACCCACCCCTGTGTGAAAAAATGCTTTGCCGGGGCCTGTCTCCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCAtgtt                                                                 <  2:536539/145‑1 (MQ=255)
                                                                                            cccgcctttttACCCACCCCCTTGTGAAAAAATGCCTCGGCGGCGGTTGTCTCCCAGTTATTTTCCCGCCCCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctg                                                  <  2:513376/143‑1 (MQ=255)
                                                                                             ccGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGAAAAAACATTTAAACCGTCATGTTTTGTggggaac                                                      >  2:206246/1‑141 (MQ=25)
                                                                                                            ccGCCGTGGGAAAAAATGCCTTCGGGGCGGTTGTCTCCCTTTTTTTTCGCCGCCAAAAAAAATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGa                                  <  1:126716/150‑1 (MQ=255)
                                                                                                               ccttgtgAAAAAATGCCTCCGCGGCGGCTGTCGGCCAGTTTTTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGaa                                 <  2:516042/146‑1 (MQ=255)
                                                                                                                  tgtgAAAAAATGCCTTCGGGGCGGTTGTCTGCCAGCTTTTTTCCCCCCAAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAggg                             <  1:215525/150‑1 (MQ=255)
                                                                                                                     gCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAGGGGAAAAAAAATTTATACCCCCCTCTTGTGTGGGGATCGCTGCTGGGGACAATATGGGAggggg                            >  1:598561/1‑147 (MQ=255)
                                                                                                                                     gcggcgGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCa              <  2:167817/148‑1 (MQ=255)
                                                                                                                                              gTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGGGAAAAAGATTTTATCCCGCCCTGTTGTGTGGGGAATGCCGCCGGTGAAAAAATGGGAAGGGGAGGGGGGCGGATCAGCTCCCGaa     >  2:258974/1‑148 (MQ=255)
                                                                                                                                                     aGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcg  <  1:163822/144‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                             
CACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCG  >  NC_000913/357724‑358014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: