Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,349,145 | 0 | A | C | 38.3% | 66.1 / 10.3 | 47 | F110V (TTC→GTC) | inaA | acid‑inducible Kdo/WaaP family putative kinase |
Reads supporting (aligned to +/- strand): ref base A (24/5); new base C (0/18); total (24/23) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.37e-09 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGAACATAACAACAGCCATGCTGACGATTAATGCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTC‑AACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCG > NC_000913/2349003‑2349290 | cGAACATAACAACAGCCATGCTGACGATTAATGCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTC‑AACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCa < 2:504804/151‑1 (MQ=255) aaCATAACAACAGCCTTGCTGACGATTAATGCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTC‑AACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATCCTGCATGGTGGGCATACCAGTCAGCATTGCTGAAGAC‑CCCCGCCat < 1:100931/150‑1 (MQ=255) aaCATAACAACAGCCATGCTGACGATTAATGCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTC‑AACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCat > 2:607984/1‑150 (MQ=255) aaaccaccaGCCATGCTGACTATTAATGTTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTC‑AACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTCCATGCTGGGCATCCCAGTCAGAATTGCTGATGAA‑CCCCGCCat < 1:452643/140‑1 (MQ=255) aaaacaacAGCCATGTTGACGATTAATGCTATGCATTTTCTAAAACGCCAGCCCCCCTGCTTCC‑AACAGGGCTTGCCGTACTTCGTAAGAATAAGGCGATCCGGCATGCTGGGCATCCCAGTCAGAAGTGCTGATGAC‑CCCCGCCATATc < 1:480012/148‑1 (MQ=255) aTAACAACAGCCATGCTGACGATTAATGCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTC‑AACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCATATCt > 2:508468/1‑151 (MQ=255) ttacaacaGCCATGCTGACGATTAATGCTATGCATATTCTTAAATGTCAGCGCCACTGCTTTC‑AACATGGCTCGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGCCAGCAATGCTGATGAA‑CCCCGCCATATCt > 1:600595/3‑150 (MQ=255) actaTTAATGCTGGTTATTTTCTAAAACCCCAGCGCCCCTGTTTAC‑AACAGGGCTTGCCTTATTTCGTCAAAAAAAGGCGATCCTCCATGCGGGGCATCCCAGCCAGCAAGGCTGAGGCA‑CCCCGCCATATCTTCAGTCACCAGCAACGc < 1:47939/147‑1 (MQ=255) aTTAATGCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTC‑AACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGc > 2:366904/1‑151 (MQ=255) ttAATGCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTC‑AACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGcc > 2:233265/1‑151 (MQ=255) tAATGCTATGCTTTTTCTAAAACCCCTCCCCCACTTTTTTC‑AATATGGTTTGCTGTTCTCCGTAAAAAAAAGGCGATCCTGCATTTTGGCCATACCACTCACAAATCCTGATGAC‑CCCCGCCATATCTTCAGTCACCAGCAAc < 1:627907/143‑1 (MQ=255) aTGCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTC‑AACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCAtt > 1:445447/1‑151 (MQ=255) tGCTTTTTCTAAAACGCCAGCGCCACTGCTTTC‑AACATGGTTGGCCGTATTTCGTCAGAATAAGGCGTTACTGCATGCGGGGCATACCAGCCAGAAATGCTGATGAC‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCAcc < 2:637274/149‑1 (MQ=255) cgCATTTTCTTAAACGCCAGCACCACTGCTCTC‑AACACGGCCTGCCGTCCTTCGTCAGAATAAAACGATACCTCATGCGGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCTCCATATCGTCAGTCAACAGCAACGCTCGCCATTCACCCt > 1:448277/2‑151 (MQ=255) cATTTTTTTAACCGCCAGCCCCCCTGCTTTC‑AACGTGGTTTCCCGTACTTCCTAAGAATAAGGCCATACTGCATTCGGGGCATACCAGCCAGTATTGCTTATGAC‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCAc < 1:499089/146‑1 (MQ=255) cATTTTCTTAAACGCCAGCGCCACTGCTTTC‑AACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTc > 2:411521/1‑150 (MQ=255) tCTTAAACGCCAGCGCCACTGCTTTC‑AACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAAtttt > 2:355148/1‑151 (MQ=255) aacccccAGCCCCCCTCCTTCC‑ACCTGGGTTTGCCGTCCTTCGTCAAAAAAGGGCTCTCCGCCGTGCTGGGAATACCAGCCAGCAATGCTGATGCC‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAAtttt < 1:607495/142‑1 (MQ=255) aCGCCAGCGCCACTGCTTTC‑AACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCACTTTTCACCGc > 1:570592/1‑151 (MQ=255) ccccACCGCCTCTGCTTAC‑ACCATGTCTTCCCGTCTTCCGTAAGAAAAAGGCTATCCTCCATGCGGGCCATCCAATTCGGAAATGCTAATAAC‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCAcc < 1:569197/146‑1 (MQ=255) cGCCAGCGCCACTGCTTTC‑AACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCgat > 1:115878/1‑149 (MQ=255) gCCAGCGCCACTGCTTTC‑AACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCtt > 2:461934/1‑151 (MQ=255) aGCGCCACTGCTTTC‑AACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGACATTCACCCTCAATTTTCACCGCTTCAc > 1:414716/1‑151 (MQ=255) gcgcCACTGCTTTC‑AACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCAcc > 2:546466/1‑151 (MQ=255) cgcCACTTCTTTA‑AACAGGGCTGGCCGTCCTTCGTCAAAAAAAGGCGATACTGCATGCTGGGCATACCAGCCAGCAATGCTGATGAC‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCa < 2:443902/151‑1 (MQ=255) ccACTGTTTTC‑AACTTGCTTTGCTTTCCTTCTTAAAAAAAAGGCTATCCTCCATGCGGGCCATACCAGTCAGCAATGCGGAGAAC‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCaa < 2:157686/150‑1 (MQ=255) cctcgCTTTCAAACATGGCCTGCCCTACTCCGACAGAAAACTGCAACCCTGCATGCTGGCCACCCCAGTCAGCAATCCTAATAAC‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTGCACCGCTTCTCCa < 1:566927/145‑1 (MQ=255) aCTGCTTTC‑AACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCaaaa > 1:109219/1‑150 (MQ=255) aaCTTGGTTTGCCTTTTTGCGTCAAAATAGGGCGATACTGCATGTGGGGAATACCAGACAGCAATGCTGATGAC‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCt < 2:126401/148‑1 (MQ=255) aaCAGGGCTTCCCTTCCTCCGTAAAAAAAAGGCGATACGGCATGCGGGGCATACCAGTCAGAAATGCTGAGGAC‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCtt < 2:599553/149‑1 (MQ=255) aggCTTGCCGTACTTAGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCa > 1:324211/2‑150 (MQ=255) cGTCCTTCGTCAAAATAGGGAGATACTGCTTGCGGGGCAACCCACTCAGAAAGGCTGATGAC‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGacac < 2:254874/151‑1 (MQ=255) ttCGTAAAAATAGGGCGATACTGCATGCTGGGCATCCCAGCCAGCAATGCTGATGAC‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCg < 2:175581/148‑1 (MQ=255) cgtaaaaaaagggCGATCCTGCATGCGGGGCATCCCAGCCAGAAATGCTGATAAC‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGccc < 2:648997/139‑1 (MQ=255) cGTAAGAATAAGGCGATACTGCATGCGGGCCATACCAGTCAGCAATGCTGATAAC‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCg < 2:357293/151‑1 (MQ=255) aGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTcc > 2:596993/1‑151 (MQ=255) ggCGTTCCTGCATGCGGGGCATCCCATTCAGAAAT‑CTGGAGAACCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCtt < 1:354767/151‑1 (MQ=255) gCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCttt > 1:52622/1‑151 (MQ=255) cTGCATGCTGGGCATACCAGTAAGCCATGCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTATTTAc > 1:614378/1‑151 (MQ=255) tGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCa > 1:450632/1‑150 (MQ=255) gCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCAAc > 2:592730/1‑151 (MQ=255) gggCATCCAAGCCAGAAATGCTGATAAC‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTc < 2:639765/151‑1 (MQ=255) gccATCCCAGTCAGCAATGCTGATGAC‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTCa < 2:433843/149‑1 (MQ=255) gccAGCAATGCTGATGCA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTCACGGACaa < 2:583739/147‑1 (MQ=255) gCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCgg > 1:576578/1‑151 (MQ=255) cTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGATCGCCATTCACCCTCATTTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACAGCAACCTCACGGACAATCGTTGGTCGGc > 1:271879/1‑151 (MQ=255) atgaA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCg > 1:260039/1‑150 (MQ=255) | CGAACATAACAACAGCCATGCTGACGATTAATGCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTC‑AACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAA‑CCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCG > NC_000913/2349003‑2349290 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |