Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,903,7150GA37.5% 66.8 / 23.0 48intergenic (+341/+1032)ygcE/queEputative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Reads supporting (aligned to +/- strand):  ref base G (4/26);  new base A (18/0);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGATTTAAGAATCATACAA  >  NC_000913/2903571‑2903858
                                                                                                                                                 |                                                                                                                                               
aTGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACTCATTATACT‑CAAAAAAAAAACaaa                                                                                                                                            >  2:490080/1‑150 (MQ=255)
  gTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACtt                                                                                                                                         <  2:3619/151‑1 (MQ=255)
      ttAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAAAAATCCTTAGCCC‑CAAAAAAAAAACaaaaat                                                                                                                                         >  2:585400/1‑144 (MQ=255)
        aGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGa                                                                                                                                    <  1:454299/150‑1 (MQ=255)
             aaaaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAGCTGGATATCTTTATTCATAAGAACTATTCATCATCAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATttatt                                                                                                                              <  1:124767/151‑1 (MQ=255)
             aaaaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATttatt                                                                                                                              <  2:573870/151‑1 (MQ=255)
               aaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACACTCATTATCCT‑CAAAAAAAAAAAAAAAATCCCGATttatt                                                                                                                              >  2:326555/1‑149 (MQ=255)
                  aCAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGCTCTTTTAAACCACTACTTATCCT‑CAAAACAAAAAAAAAACTCCaaggtttat                                                                                                                              >  1:142251/1‑138 (MQ=255)
                        cGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAAAAACCCTTAACCT‑CAAAAAAAAAAAAAACCTCCCAAtttttttatttaacaa                                                                                                                    >  2:246581/1‑140 (MQ=255)
                         gAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTGTTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATg                                                                                                                  <  2:685769/151‑1 (MQ=255)
                         gAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACACTCATTAGCCT‑CCAAAAAAAAAAAAAAATCCAGATTTATTACTTAACCaa                                                                                                                    >  2:499791/1‑149 (MQ=255)
                          aaTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAAAAACCATTAGACC‑CAAAAAAAAAACAAAATTTCAGATTTATTTTTTTACCaa                                                                                                                    >  2:548967/1‑148 (MQ=255)
                             cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAAt                                                                                                               <  1:487432/150‑1 (MQ=255)
                                     aTCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACACATTAAACT‑CCAAAAAAAAAAAAACTTCCAGAATTATTTATTTACCCAAGAAAAAAAAACtt                                                                                                      >  2:567024/1‑151 (MQ=255)
                                       cAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAAAACCCATTTAACT‑CAAAAAAAAAAAAAACCTCCCAATTTTTTTCTTTACCAAAGaaaaaaaaacct                                                                                                      >  2:240830/1‑145 (MQ=255)
                                               aCTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTATAAACACTCCTTAAACT‑CAAAACAAAGAAAAAATTTCAGATTTTTTAATTAAACAAAGAATAAAAAACCTAAAAAaaat                                                                                             >  1:27338/1‑150 (MQ=255)
                                                cTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTATAACCACTCATTAGCCT‑CAAAACAAAGATAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAatat                                                                                             <  1:630777/149‑1 (MQ=255)
                                                    aaGTATGAACTAGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGAGTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGtt                                                                                       <  1:483243/151‑1 (MQ=255)
                                                      gTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTAt                                                                                     <  2:19186/151‑1 (MQ=255)
                                                        aTGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTAtt                                                                                    <  2:625929/150‑1 (MQ=255)
                                                        aTGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACACTCCTTAAACT‑CAAAAAAAAAACAAAATTCCAGATTTATTTCTTTAACAATGAAAAAAAAACATTAAAAAAATAATGTtttt                                                                                    >  2:546660/1‑150 (MQ=255)
                                                         tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAACAAAACATTATCCC‑CAAAAAAAAAAAAAAATTTAACAGTTATTATATTACCAATAAATAAAAAAATTAAAAAATAAAATGTtttt                                                                                    >  1:630128/1‑149 (MQ=255)
                                                          gAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTTAAAAAAATCATTAGACT‑TAAAAAAAAAAAAAAACTTCACATTTATTTCTTAACACATAAAAAAAAAAACTAAAAAataaaatttttttt                                                                                   >  1:517661/1‑138 (MQ=255)
                                                            aCTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTaaa                                                                               <  1:532895/151‑1 (MQ=255)
                                                                  tatCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACAAATTATCCT‑TCAAACAAAAACAAAAATACAAATTTTTTTCTTTAACAATTAAAAACAAACTTCAAAAAAATAATGTTTTTTTTAaaaaaa                                                                          >  1:122934/1‑150 (MQ=255)
                                                                   atCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGaaaaa                                                                         <  2:615511/150‑1 (MQ=255)
                                                                   atCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGCTATGTAAAAACAACCATTATTCTCCAAAAAAAAAAAAAATTTCCCAATTT‑TTAATTAAACAATAAAAAAACAACTTTAAAAAAATAATTTTTTTCTTAACaaaaa                                                                         >  2:396275/1‑150 (MQ=255)
                                                                      tttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACCCATTAACCT‑CAAAAAAAAAAAAAAATTTCCGATTTTTTTATTAAACAAAGAAAAAAAAACTTAAAAAAAATAATGTTTTTTTaaaaaaaaaa                                                                        >  2:203509/1‑148 (MQ=255)
                                                                        tATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTGAAAAACACTCAATATCCC‑CAAAAAAAAAAAAAAAATCCAAATTTTTTATTTAAACAAAAAAAAAAAAAATTAAAAAAAAAAATTTTTTTTTTAAAAAAAAAATa                                                                     >  1:323635/1‑149 (MQ=255)
                                                                        tATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAAAGAATAACAAGCTTAAAAAATAAACTGTTATTCTAAAGAAAAAAtt                                                                      <  1:116782/148‑1 (MQ=255)
                                                                           tCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAg                                                                 <  2:104467/150‑1 (MQ=255)
                                                                             atgaGAACTATACATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACa                                                                  <  2:681639/144‑1 (MQ=255)
                                                                               aaGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCAt                                                             <  2:513724/150‑1 (MQ=255)
                                                                                aGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTa                                                           <  2:132023/151‑1 (MQ=255)
                                                                                   aCTATTCATCAACAGCGTATAGAGGCGGTTATGTAAATCCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTaa                                                          <  1:646542/149‑1 (MQ=255)
                                                                                       ttCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACCCCTTTATCC‑CAAAAAAAAAAAAAAATTTCAAATTTATTACCTAAACAATTAAAAAAAAAAATAAAAAAACTACTTTTTATTTTAACAAAAAAATAAAAAGCAATaa                                                          >  1:471623/1‑145 (MQ=255)
                                                                                         catcaACAGCGTATAGAGGCGGTTATGTAAAACCACACATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACGTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAata                                                   <  1:400408/150‑1 (MQ=255)
                                                                                                     ataGAGGCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaataa                                       <  2:620311/150‑1 (MQ=255)
                                                                                                       agagGCGGTTATGTAAAACCACCCATTAGCCT‑CCAAAAAAAAACAAACATCCAGGTTTATTACTTAAACAACGAATAAAAAACCTAAAAAAAATAATTTTATTTTAAAAAAAAAAAAAAAAAGCAATAATTAAATAATaaaaaaaaaa                                       >  1:116700/1‑148 (MQ=255)
                                                                                                         aagCGGTTATGTAAAACTACTCATTAGCCT‑CAAAACAAAGACAAACTACCACATTTATTTCTTAACCAATAAATAACAACTTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACATTCATTAATTCAATATTAAAAAAATAATAgg                                   <  1:138358/148‑2 (MQ=255)
                                                                                                           gCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGAtt                                 >  1:85104/1‑150 (MQ=255)
                                                                                                           gCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTa                                <  2:113478/151‑1 (MQ=255)
                                                                                                            cGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaa                               <  2:137474/151‑1 (MQ=255)
                                                                                                             ggTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaaa                              <  2:683223/151‑1 (MQ=255)
                                                                                                                              cATTAGCCT‑CCAAACACAGACAAAATTCCAGATTTATTACTTAACCCATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAACATAATAGATTAAAATTTCTTAACGATTTa              >  1:644630/1‑150 (MQ=255)
                                                                                                                                      t‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGAGTAAAATTTCTTAACGATTTAAg            <  1:629351/144‑1 (MQ=255)
                                                                                                                                          aaaCAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGATTTAAGAATCATACaa  <  1:326358/151‑1 (MQ=255)
                                                                                                                                          aaaCAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGATTTAAGAATCATACa   >  1:78390/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
ATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGATTTAAGAATCATACAA  >  NC_000913/2903571‑2903858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑