Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,903,7150GA37.5% 66.8 / 23.0 48intergenic (+341/+1032)ygcE/queEputative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Reads supporting (aligned to +/- strand):  ref base G (4/26);  new base A (18/0);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGATTTAAGAATCATACAA  >  NC_000913/2903571‑2903858
                                                                                                                                                 |                                                                                                                                               
aTGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACTCATTATACTCAAAAAAAAAACaaa                                                                                                                                            >  2:490080/1‑150 (MQ=255)
  gTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACtt                                                                                                                                         <  2:3619/151‑1 (MQ=255)
      ttAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAAAAATCCTTAGCCCCAAAAAAAAAACaaaaat                                                                                                                                         >  2:585400/1‑144 (MQ=255)
        aGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGa                                                                                                                                    <  1:454299/150‑1 (MQ=255)
             aaaaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAGCTGGATATCTTTATTCATAAGAACTATTCATCATCAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATttatt                                                                                                                              <  1:124767/151‑1 (MQ=255)
             aaaaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATttatt                                                                                                                              <  2:573870/151‑1 (MQ=255)
               aaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACACTCATTATCCTCAAAAAAAAAAAAAAAATCCCGATttatt                                                                                                                              >  2:326555/1‑149 (MQ=255)
                  aCAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGCTCTTTTAAACCACTACTTATCCTCAAAACAAAAAAAAAACTCCaaggtttat                                                                                                                              >  1:142251/1‑138 (MQ=255)
                        cGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAAAAACCCTTAACCTCAAAAAAAAAAAAAACCTCCCAAtttttttatttaacaa                                                                                                                    >  2:246581/1‑140 (MQ=255)
                         gAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTGTTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATg                                                                                                                  <  2:685769/151‑1 (MQ=255)
                         gAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACACTCATTAGCCTCCAAAAAAAAAAAAAAATCCAGATTTATTACTTAACCaa                                                                                                                    >  2:499791/1‑149 (MQ=255)
                          aaTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAAAAACCATTAGACCCAAAAAAAAAACAAAATTTCAGATTTATTTTTTTACCaa                                                                                                                    >  2:548967/1‑148 (MQ=255)
                             cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAAt                                                                                                               <  1:487432/150‑1 (MQ=255)
                                     aTCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACACATTAAACTCCAAAAAAAAAAAAACTTCCAGAATTATTTATTTACCCAAGAAAAAAAAACtt                                                                                                      >  2:567024/1‑151 (MQ=255)
                                       cAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAAAACCCATTTAACTCAAAAAAAAAAAAAACCTCCCAATTTTTTTCTTTACCAAAGaaaaaaaaacct                                                                                                      >  2:240830/1‑145 (MQ=255)
                                               aCTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTATAAACACTCCTTAAACTCAAAACAAAGAAAAAATTTCAGATTTTTTAATTAAACAAAGAATAAAAAACCTAAAAAaaat                                                                                             >  1:27338/1‑150 (MQ=255)
                                                cTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTATAACCACTCATTAGCCTCAAAACAAAGATAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAatat                                                                                             <  1:630777/149‑1 (MQ=255)
                                                    aaGTATGAACTAGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGAGTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGtt                                                                                       <  1:483243/151‑1 (MQ=255)
                                                      gTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTAt                                                                                     <  2:19186/151‑1 (MQ=255)
                                                        aTGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTAtt                                                                                    <  2:625929/150‑1 (MQ=255)
                                                        aTGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACACTCCTTAAACTCAAAAAAAAAACAAAATTCCAGATTTATTTCTTTAACAATGAAAAAAAAACATTAAAAAAATAATGTtttt                                                                                    >  2:546660/1‑150 (MQ=255)
                                                         tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAACAAAACATTATCCCCAAAAAAAAAAAAAAATTTAACAGTTATTATATTACCAATAAATAAAAAAATTAAAAAATAAAATGTtttt                                                                                    >  1:630128/1‑149 (MQ=255)
                                                          gAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTTAAAAAAATCATTAGACTTAAAAAAAAAAAAAAACTTCACATTTATTTCTTAACACATAAAAAAAAAAACTAAAAAataaaatttttttt                                                                                   >  1:517661/1‑138 (MQ=255)
                                                            aCTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTaaa                                                                               <  1:532895/151‑1 (MQ=255)
                                                                  tatCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACAAATTATCCTTCAAACAAAAACAAAAATACAAATTTTTTTCTTTAACAATTAAAAACAAACTTCAAAAAAATAATGTTTTTTTTAaaaaaa                                                                          >  1:122934/1‑150 (MQ=255)
                                                                   atCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGaaaaa                                                                         <  2:615511/150‑1 (MQ=255)
                                                                   atCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGCTATGTAAAAACAACCATTATTCTCCAAAAAAAAAAAAAATTTCCCAATTTTTAATTAAACAATAAAAAAACAACTTTAAAAAAATAATTTTTTTCTTAACaaaaa                                                                         >  2:396275/1‑150 (MQ=255)
                                                                      tttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACCCATTAACCTCAAAAAAAAAAAAAAATTTCCGATTTTTTTATTAAACAAAGAAAAAAAAACTTAAAAAAAATAATGTTTTTTTaaaaaaaaaa                                                                        >  2:203509/1‑148 (MQ=255)
                                                                        tATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTGAAAAACACTCAATATCCCCAAAAAAAAAAAAAAAATCCAAATTTTTTATTTAAACAAAAAAAAAAAAAATTAAAAAAAAAAATTTTTTTTTTAAAAAAAAAATa                                                                     >  1:323635/1‑149 (MQ=255)
                                                                        tATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAAAGAATAACAAGCTTAAAAAATAAACTGTTATTCTAAAGAAAAAAtt                                                                      <  1:116782/148‑1 (MQ=255)
                                                                           tCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAg                                                                 <  2:104467/150‑1 (MQ=255)
                                                                             atgaGAACTATACATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACa                                                                  <  2:681639/144‑1 (MQ=255)
                                                                               aaGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCAt                                                             <  2:513724/150‑1 (MQ=255)
                                                                                aGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTa                                                           <  2:132023/151‑1 (MQ=255)
                                                                                   aCTATTCATCAACAGCGTATAGAGGCGGTTATGTAAATCCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTaa                                                          <  1:646542/149‑1 (MQ=255)
                                                                                       ttCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACCCCTTTATCCCAAAAAAAAAAAAAAATTTCAAATTTATTACCTAAACAATTAAAAAAAAAAATAAAAAAACTACTTTTTATTTTAACAAAAAAATAAAAAGCAATaa                                                          >  1:471623/1‑145 (MQ=255)
                                                                                         catcaACAGCGTATAGAGGCGGTTATGTAAAACCACACATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACGTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAata                                                   <  1:400408/150‑1 (MQ=255)
                                                                                                     ataGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaataa                                       <  2:620311/150‑1 (MQ=255)
                                                                                                       agagGCGGTTATGTAAAACCACCCATTAGCCTCCAAAAAAAAACAAACATCCAGGTTTATTACTTAAACAACGAATAAAAAACCTAAAAAAAATAATTTTATTTTAAAAAAAAAAAAAAAAAGCAATAATTAAATAATaaaaaaaaaa                                       >  1:116700/1‑148 (MQ=255)
                                                                                                         aagCGGTTATGTAAAACTACTCATTAGCCTCAAAACAAAGACAAACTACCACATTTATTTCTTAACCAATAAATAACAACTTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACATTCATTAATTCAATATTAAAAAAATAATAgg                                   <  1:138358/148‑2 (MQ=255)
                                                                                                           gCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGAtt                                 >  1:85104/1‑150 (MQ=255)
                                                                                                           gCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTa                                <  2:113478/151‑1 (MQ=255)
                                                                                                            cGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaa                               <  2:137474/151‑1 (MQ=255)
                                                                                                             ggTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaaa                              <  2:683223/151‑1 (MQ=255)
                                                                                                                              cATTAGCCTCCAAACACAGACAAAATTCCAGATTTATTACTTAACCCATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAACATAATAGATTAAAATTTCTTAACGATTTa              >  1:644630/1‑150 (MQ=255)
                                                                                                                                      tCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGAGTAAAATTTCTTAACGATTTAAg            <  1:629351/144‑1 (MQ=255)
                                                                                                                                          aaaCAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGATTTAAGAATCATACaa  <  1:326358/151‑1 (MQ=255)
                                                                                                                                          aaaCAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGATTTAAGAATCATACa   >  1:78390/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
ATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGATTTAAGAATCATACAA  >  NC_000913/2903571‑2903858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: