Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT39.3% 27.8 / 11.1 28intergenic (‑179/‑28)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (3/14);  new base T (11/0);  total (14/14)
Fisher's exact test for biased strand distribution p-value = 3.39e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG  >  NC_000913/3206291‑3206585
                                                                                                                                                 |                                                                                                                                                       
aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACa                                                                                                                                                   <  1:268115/151‑1 (MQ=255)
            aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACt                                                                                                                                       <  1:161788/151‑1 (MQ=255)
               gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCA‑ACAaaaaaaaaaaaagg                                                                                                                                     >  2:574254/1‑146 (MQ=255)
                         gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCatga                                                                                                                         <  2:40897/151‑1 (MQ=255)
                                   aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAATTGAG‑GTGTCATGGTGAGCGaaa                                                                                                                >  1:645497/1‑150 (MQ=255)
                                                aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAg                                                                                                  <  1:174125/151‑1 (MQ=255)
                                                   ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAAAACAAAAAATGGGG‑GTGGCATGATGAGGGAAAGGAAAAAACaa                                                                                                     >  2:537798/1‑145 (MQ=255)
                                                       cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGGG‑GTTGCATGAAGAGCGAAAAGAATAAACAACAAGCaagg                                                                                            >  2:617127/1‑147 (MQ=255)
                                                        gTTGATACACGAACTGTGAATAACATATGCCGGGTGGCAAAGAGTATAAAGTCAGAGTTTTAGTGGTAGTTTGTGGCGGTG‑AGGTTTTCCAAACAAATACCAAAACTGAAG‑TTGCCATGATGCGCGAACTTAATAAGCAACAGGCAgcg                                                                                            <  1:80175/149‑3 (MQ=255)
                                                          tGATTCACGAACTGCTAATTACCTATTCACGGTGGCAAACAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCG‑AATTCTTCACGCCCAATATAAAAAATTGGG‑TTGCCATACTGAGCGAAACCAACAAGCAAAAgggcgt                                                                                             >  1:584747/1‑142 (MQ=255)
                                                           gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                                        <  2:241759/150‑1 (MQ=255)
                                                               cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGtt                                                                                    <  1:505197/150‑1 (MQ=255)
                                                                  gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa                                                                                <  1:265113/151‑1 (MQ=255)
                                                                    aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAG‑AAATTTTTCAGACAAATTCAAAAACTGGAG‑TTTGCATGATGAGCGTAAGTAAAAAAGAACAAGCAGTGAATa                                                                                        >  1:127533/1‑141 (MQ=255)
                                                                            ttACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATTGAG‑GTGCCATGATGAGCGAAAATAATAAAAAACAAGCAGGGAATAAGTTTACAGAGAtt                                                                          >  2:327985/1‑147 (MQ=255)
                                                                              aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAAATGGAG‑GTGCCCTGATGAGGGAAAGTAATAAACAACAGGCAGGGAATAAGTTGACAGAGATTGTCg                                                                      >  2:478628/1‑149 (MQ=255)
                                                                                atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGAAAAAAAAAAAAACCGGAGATTCCCAT‑ATGAGCGGAAGCAATAAACAAAAAGCAGTGAAAAAAATGACAAAGGTTGgcgc                                                                     >  2:606909/1‑148 (MQ=255)
                                                                                   ttCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAG‑TTGCCCTGATGAGCGAAAATAATAAACAACAGGCAGTGAAAAAATTGACAGAGATTGTCGCTAACtt                                                               >  2:440403/1‑151 (MQ=255)
                                                                                      aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAG‑GTGCCATGATTAGCGAAAAAAATAAACAAAAAGCAGGGAAAAAGGTGAAAGAGAATGTCGCTAAAttt                                                              >  2:568226/1‑149 (MQ=255)
                                                                                            gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCCGACAAAAACAAAAAATGGAG‑TTGCCCTGATGAGCGAAAATAAAAAACAACAAGCAGGGAATAAGTTGACAGAGATTGTTGCCAACTTTACCGCCAt                                                      >  2:300497/1‑151 (MQ=255)
                                                                                             cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAG‑GTGCCATGATGAGGGAAAGGAATAAGCAAAAGGCAGTGAATAAGTTGAAAGAGATTGTCGCTAACTTTAACGcc                                                        >  2:473381/1‑148 (MQ=255)
                                                                                                             acaTTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGc                                     <  2:553370/151‑1 (MQ=255)
                                                                                                              caTTTTTAGTGGTGGTTTGTGGCGGTG‑AATTTTTCAAGACAAAAACAAAAACTGGAG‑TTCCCATGATGAGCGAAAGTAATAAGCAACGGGCAGTAAATAAGTGGACAGAGATTGTAGTTACCTTTACCGCCATGATTTCTACCCGaa                                        <  1:454338/147‑1 (MQ=255)
                                                                                                                       tGGTAGTTTGGGGCGGTG‑AATTTTTCCAGACAAATACAAAACCTGGAG‑TTGCCATGAGGAGCAAAATAAAAAAGCAACAGGCAGTGAAAAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatga                              <  1:263754/148‑1 (MQ=255)
                                                                                                                                gtgGCGGTG‑AATTTTCCCAGAAAAATAAAAAAACGGGAG‑TTGCCATGATGAGCAAAAGTAAAAAGAAACAGGAATTGATTAGGTTGACAGGGATTGTCGCAAACTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                     <  2:627174/148‑1 (MQ=255)
                                                                                                                                   gggggg‑AATTTTTCCAAACAATGAAAAAACGTGAAG‑TTCCAGTGATGAGCGAAATAAATAGGCTACAGCCAGTAAATAAGTGAAAGGAGATTGTCGCTAACTTGACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt                    <  1:608119/141‑1 (MQ=255)
                                                                                                                                     ggTG‑AATTTTTCAAGAAAAATACAAAAGCGGGAG‑TTGCCAGGAGAAGAGAAAGAAATAAGCAACAGGAAGGCAATAAGTTGACAGAAATGGTCGTTACCTTTGCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa              <  2:61433/150‑1 (MQ=255)
                                                                                                                                       tG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa            >  2:492304/1‑150 (MQ=255)
                                                                                                                                                 ccAGACAAATAAAAAAACGGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACGGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAg  <  2:314769/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                       
ATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG  >  NC_000913/3206291‑3206585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑