Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,538,1090AC42.0% 74.1 / 14.9 51G219G (GGT→GGGnanMN‑acetylneuraminic acid mutarotase
Reads supporting (aligned to +/- strand):  ref base A (26/3);  new base C (0/21);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 3.01e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTT  >  NC_000913/4537962‑4538257
                                                                                                                                                    |                                                                                                                                                     
ccccGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCGCCt                                                                                                                                                    <  2:135960/151‑1 (MQ=255)
     cAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTAtt                                                                                                                                              >  2:215515/1‑151 (MQ=255)
       aaaCCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATACTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTc                                                                                                                                             >  1:145961/1‑150 (MQ=255)
              cAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTTGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAAccgc                                                                                                                                     <  1:569318/151‑1 (MQ=255)
              cAGCTACGCCATCTGGTGATGACACGGGAGCAAGCTTTTTCGATTTTAAATTATTAAGGGTGAAATCAAGTTCAAATACGGCAGCCGTTCGAAATACTGGTATGGCTTCACCATTAATAAGCAAGGTTTTATGACCTTTATTTACAAc                                                                                                                                        >  1:288063/1‑148 (MQ=255)
                        aTCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGcc                                                                                                                           >  2:258457/1‑151 (MQ=255)
                            ggTGAGGCGGCGGGGGCAAGTTTTTCCCATTTTAATTTATACCCGGTGAAACAAAGTTCAAATACCGCTCCCGTCCCCAGTCGTGTTTTGTCCCCCCCCTTAATGCGGCATGTTTTACCCCGTCTTTTCACAACCGCCGCACCAGcc                                                                                                                           <  1:37293/147‑1 (MQ=255)
                               gatgaGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGt                                                                                                                    >  2:23275/1‑151 (MQ=255)
                                  gagaCGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTAcc                                                                                                                 >  1:342736/1‑151 (MQ=255)
                                       gggAGCAAGTTTATTCCTTTTAAAATTATTCCCGGTGAAATCAAGTTCAAATACGGCACCCGTTCGCAACCCTGGTTTGGCTTCCCCATTAATAAGCCAGTTTTTACCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAg                                                                                                               <  2:610266/148‑1 (MQ=255)
                                       gggAGCAAGCTTATTCCATTTTAAATTATTCCCGTTGAAATCAAGTTCAAATACGGCACCCGTTCGCAATCCTGTTTTGGCTTCCCCATTAATAAGCCAGGTTTTATCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGc                                                                                                            <  2:377739/151‑1 (MQ=255)
                                       gggAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAggg                                                                                                             >  1:287222/1‑150 (MQ=255)
                                            cAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTc                                                                                                       >  2:77545/1‑151 (MQ=255)
                                              aGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGc                                                                                                     >  2:5600/1‑151 (MQ=255)
                                                      ccATTTTAAATTATTACCGGTGAAATCACGTTCAAATACGGCATCCGTTCGCAATACTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACTTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCTGGGCGATTCGCCAGCGTa                                                                                              >  1:375338/1‑150 (MQ=255)
                                                       cATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAAc                                                                                            >  2:369934/1‑151 (MQ=255)
                                                        aTTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACt                                                                                           >  2:486772/1‑151 (MQ=255)
                                                         ttttAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTc                                                                                          >  2:47062/1‑151 (MQ=255)
                                                          tttATATTCTCACCGGTGAAATCAAGTTCCAATAAGGCGTCCGTTTGCAATCCTGGTCTGTCTCCGCTCTTACTCATCCAGGTTTTATCACCTTTATTCACAATCGCCGCTCCAGCCGTGCCGTAGCAGGGTGATTCGCGAGCGTAACtac                                                                                         >  1:293799/1‑149 (MQ=255)
                                                           ttAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTcc                                                                                         >  1:409057/1‑150 (MQ=255)
                                                            tAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCAt                                                                                       >  1:79258/1‑151 (MQ=255)
                                                                   ttccgggTAAAATCAATTTAAAATCCGGCTCCCGTTCGAAATCCTGGTTTGGTTTCGCCATAAAAAAGCCAGTTTTTATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtgt                                                                                 <  2:532290/145‑1 (MQ=255)
                                                                    taCCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTgtgt                                                                               >  2:42474/1‑151 (MQ=255)
                                                                      ccGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTATCTCCATTGCTGTGTTg                                                                             >  2:197073/1‑151 (MQ=255)
                                                                      ccGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGtt                                                                              >  1:215271/1‑150 (MQ=255)
                                                                                 aaGTTCAAATCCGGCTCCCGTTCGCATTCCTGTTTTGGCTTCGCCATAAATAAGCCAGTTTTTACCCCCTTTATCCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaa                                                                    <  2:189921/149‑1 (MQ=255)
                                                                                  atttaaaaTACGCCACCCTTTCCCAACCCTGTTTTGGTTTCCCCATTAAAAAGCCAGTTTTTACCCCTTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAg                                                                 <  1:342678/146‑1 (MQ=255)
                                                                                       aaatcgggCCTCCTTTCGCATCCTTGTTTTGGTTTCGCAATTAAAAAGCCGGTTTTTACCCCCTTTATCCCCAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGacaac                                                            <  2:7089/145‑1 (MQ=255)
                                                                                             ggCATCCTTTCACAATCCTGGTTTGGCTTCCCCATTAATAAGCCAGTTTTTATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGaa                                                        <  2:157413/149‑1 (MQ=255)
                                                                                                aTCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACtt                                                    >  1:585041/1‑150 (MQ=255)
                                                                                                aTCAGTTCGCAATCCTGGATTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACAAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACtt                                                    >  1:449593/1‑150 (MQ=255)
                                                                                                  ctgTTCGCAACCTTGGTTGGGCTCCGCCATAAAAAACCCATTTTTTCCCCCCTTAATACACCCCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTa                                                   <  1:539844/147‑1 (MQ=255)
                                                                                                          aaTCCTGTTTTGGCTTCCCCATTAAAAGGCCAGGTTTTATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTa                                                   <  2:563192/141‑1 (MQ=255)
                                                                                                          aaTCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaat                                         >  2:395851/1‑151 (MQ=255)
                                                                                                               tgttttGGTTTCGCCTTTAATAACCCAGGTTTTATCCCCTTTATACACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaataat                                      <  2:243996/146‑1 (MQ=255)
                                                                                                                 ggttGGCTTCCGCCATAAAAAGGCTGGTTTTTCCCCCTTTAATCCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaaga                                            <  1:110826/140‑1 (MQ=255)
                                                                                                                        ttCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtttt                           >  1:73207/1‑151 (MQ=255)
                                                                                                                           cGCCATTAATAAGCCAGTTTTTATCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtttttt                         <  2:653696/151‑1 (MQ=255)
                                                                                                                            gCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTg                        >  2:600083/1‑151 (MQ=255)
                                                                                                                                ttaaaacgccgttttttATCCCTTTTTTTCCCAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCt                              <  1:77520/128‑1 (MQ=255)
                                                                                                                                ttAATAAGCCAGGTTTTATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTg                        <  2:263089/147‑1 (MQ=255)
                                                                                                                                     acgCCGGGTTTTACCCCTTTTTTCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAg               <  1:109130/149‑1 (MQ=255)
                                                                                                                                     acccctgtttttAACCCCTTTTTACACAGCCGCCTCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCaaaa                  <  1:79371/140‑1 (MQ=255)
                                                                                                                                      accaattttttcacACCTTTATCAAAACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATa                <  1:448489/136‑1 (MQ=255)
                                                                                                                                        ccAGTTTTTCCCCCCTTTTTTACCAACCGCCGCCCCAGCCGTTCCGTACCAGGGAGATTCGCCAGCGTAACTCCATTGCTGTATTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATa                <  2:275660/147‑1 (MQ=255)
                                                                                                                                        ccAGTTTTTATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAg               <  2:231300/148‑1 (MQ=255)
                                                                                                                                         catgTTTTACCCCCTTTATCCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGa            <  2:239492/147‑1 (MQ=255)
                                                                                                                                           gtttttACCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGc          <  2:575457/148‑1 (MQ=255)
                                                                                                                                             ttttATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCAtt       >  2:457064/1‑151 (MQ=255)
                                                                                                                                              tttATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATACTCTTCTGCTTTTTTGTCAAAATAGTGAGCAtt       >  1:573035/1‑150 (MQ=255)
                                                                                                                                                   cACTTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAttt  <  1:544162/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTT  >  NC_000913/4537962‑4538257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: