Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	318,484	C→T	L28L (CTG→CTA)	rclB ←	reactive chlorine species (RCS) stress resistance periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	318,484	0	C	T	100.0%	189.1 / NA	51	L28L (CTG→CTA)	rclB	reactive chlorine species (RCS) stress resistance periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/29);  total (22/29)

ATACAATACTGCTGTTGCGGTTGAATTATTATCAATTCGCATCGATGTGACTTTCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTCAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGAT  >  NC_000913/318337‑318626                                                                                                                                                    |                                                                                                                                               ataCAATACTGCTGTTGCGGTTGAATTATTATCAATTCGCATCGATGTGACTTTCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAg                                                                                                                                              <  1:675779/150‑1 (MQ=255) ataCAATACTGCTGTTGAGGTTGAATTATTATCAATTCGCATCGATGTGACTTTCCAGGATGAAGCATTATATTTTCTGTCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAg                                                                                                                                              <  2:510732/150‑1 (MQ=255)     aaTACTGCTGTTGCGGTTGAATTATTATCAATTCGCATCGATGTGACTTTCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAatta                                                                                                                                         <  2:146912/151‑1 (MQ=255)      aTACTGCTGTTGCGGTTGAATTATTATCAATTCGCATCGATGTGACTTTCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAattat                                                                                                                                        >  1:78286/1‑151 (MQ=255)         ctgctgTTGCGGTTGAATTATTATCAATTCGCATCGATGTGACTTTCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGCACTGTCTTGGACCCTGATACTTAGGAGAGGGAGATATGTGTTAGAATGAtttt                                                                                                                                     >  2:49202/1‑151 (MQ=255)           gctgTTGCGGTTGAATTATTATCAATTCGCATCGATGTGACTTTCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTAtttt                                                                                                                                     <  1:566733/149‑1 (MQ=255)               ttGCGGTTGAATTATTATCAATTCGCATCGATGTGACTTTCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTatcatc                                                                                                                               >  2:354849/1‑151 (MQ=255)                tGCGGTTGAATTATTATCAATTCGCATCGATGTGACTTTCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTatcatc                                                                                                                               <  1:286631/150‑1 (MQ=255)                tGCGGTTGAATTATTATCAATTCGCATCGATGTGACTTTCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCt                                                                                                                              >  2:523257/1‑151 (MQ=255)                tGCGGTTGAATTATTATCAATTCGCATCGATGTGACTTTCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCt                                                                                                                              >  2:49836/1‑151 (MQ=255)                    gTTGAATTATTATCAATTCGCATCGATGTGACTTTTCAGGATGAAGCATTATATTTTCTGGCTATATCAGCACTGGTATCCTCCAGAACTGTAGGTGATGCTGATACTGACGAGACGCTGATATGATTTAGAATTATTTTATCATCTGCa                                                                                                                           >  1:406778/1‑150 (MQ=255)                      tGAATTATTATCAATTCGCATCGATGTGACTTTCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCAtt                                                                                                                         <  2:235968/150‑1 (MQ=255)                               atCAATTCGCATCGATGTGACTTTCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAAt                                                                                                               <  1:253223/151‑1 (MQ=255)                                    ttCGCATCGATGTGACTTTCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCAt                                                                                                          >  1:121214/1‑151 (MQ=255)                                      cGCATCGATGTGACTTTCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATa                                                                                                         <  2:629644/150‑1 (MQ=255)                                         aTCGATGTGACTTTCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGc                                                                                                     >  2:278368/1‑151 (MQ=255)                                           cGATGTGACTTTCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCa                                                                                                   <  1:217720/151‑1 (MQ=255)                                               gtgACTTTCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGat                                                                                                <  1:281683/150‑1 (MQ=255)                                                  aCTTTCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGataat                                                                                             >  1:298111/1‑150 (MQ=255)                                                     ttCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAg                                                                                           <  1:193987/149‑1 (MQ=255)                                                        cAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTg                                                                                      <  2:411438/151‑1 (MQ=255)                                                         aGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGc                                                                                     <  2:572045/151‑1 (MQ=255)                                                          ggATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCa                                                                                    <  1:162984/151‑1 (MQ=255)                                                              gAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAAt                                                                                 >  2:494873/1‑150 (MQ=255)                                                               aaGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATaa                                                                               <  2:650512/151‑1 (MQ=255)                                                                 gCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATaaaa                                                                             <  2:293487/151‑1 (MQ=255)                                                                  cATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAAc                                                                            <  2:158060/151‑1 (MQ=255)                                                                    ttATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTTATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAAc                                                                            >  1:216165/1‑149 (MQ=255)                                                                     tataTTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAAc                                                                            >  2:408600/1‑148 (MQ=255)                                                                     tataTTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAg                                                                          <  2:612735/150‑1 (MQ=255)                                                                          tttCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGAttttt                                                                    >  1:455212/1‑151 (MQ=255)                                                                              tGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTaa                                                                 >  2:273188/1‑150 (MQ=255)                                                                               ggCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTa                                                                  <  2:550761/148‑1 (MQ=255)                                                                               ggCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGAGATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTGGCAAAGAAAGGAGATTTTTTag                                                                 <  1:283467/149‑2 (MQ=255)                                                                                   atatCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACatat                                                           >  1:418940/1‑151 (MQ=255)                                                                                           aaTGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATAcc                                                   <  2:543321/151‑1 (MQ=255)                                                                                             tGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCAt                                                 >  1:541635/1‑151 (MQ=255)                                                                                              ggTATCCTCCAGAACTGTCGGTGGTGCTGATACTGACGAGAGGCTGATATGTTTTAGAATTATTTTATCAACAGCATTGGTGGGAAATGCCAGAACTACAGATGATAGTGTTACAAATAAATCAGATTGCTTAAGGATATGATATACGATc                                                <  1:604511/151‑1 (MQ=255)                                                                                              ggTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCAt                                                 <  1:641333/150‑1 (MQ=255)                                                                                                tATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCa                                               >  1:309341/1‑150 (MQ=255)                                                                                                          aaCTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGAtt                                    >  1:66604/1‑151 (MQ=255)                                                                                                            cTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTa                                   >  1:279932/1‑150 (MQ=255)                                                                                                              gTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTaag                                 <  1:403350/150‑1 (MQ=255)                                                                                                                cGGTGATGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGAGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTaagaag                              <  1:495165/151‑1 (MQ=255)                                                                                                                     aTGCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTtata                         <  2:530391/151‑1 (MQ=255)                                                                                                                       gCTGATACTGACGAGACGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATaga                       <  2:287689/151‑1 (MQ=255)                                                                                                                                   gagaCGCTGATATGTTTTAGAATTATTTTATCCTCTGCATTGGAGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGaa            >  1:29424/1‑150 (MQ=255)                                                                                                                                    agaCGCTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAAtt          <  2:450037/151‑1 (MQ=255)                                                                                                                                         cTGATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATtaata      >  2:579896/1‑150 (MQ=255)                                                                                                                                           gATATGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATtaata      >  2:542043/1‑148 (MQ=255)                                                                                                                                            atatGTTTTAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGAt  <  2:30808/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                               ATACAATACTGCTGTTGCGGTTGAATTATTATCAATTCGCATCGATGTGACTTTCCAGGATGAAGCATTATATTTTCTGGCTATATCAGCAATGGTATCCTCCAGAACTGTCGGTGATGCTGATACTGACGAGACGCTGATATGTTTCAGAATTATTTTATCATCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGAT  >  NC_000913/318337‑318626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑