Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,728,882	A→C	Q179P (CAG→CCG)	rnt →	RNase T; exoribonuclease T; structured DNA 3' exonuclease; RNA processing; DNA repair

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,728,882	0	A	C	100.0%	138.9 / NA	40	Q179P (CAG→CCG)	rnt	RNase T; exoribonuclease T; structured DNA 3' exonuclease; RNA processing; DNA repair
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/18);  total (22/18)

TGGCCGCCGCAGAACGCGCCTCACTGAAACGTAACCCGTTCCACCCTTTCGCCACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGG  >  NC_000913/1728735‑1729029                                                                                                                                                    |                                                                                                                                                    tgGCCGCCGCAGAACGCGCCTCACTGAAACGTAACCCGTTCCACCCTTTCGCCACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGgc                                                                                                                                                  >  2:59177/1‑151 (MQ=255)  ggCCGCCGCAGAACGCGCCTCACTGAAACGTAACCCGTTCCACCCTTTCGCCACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGgc                                                                                                                                                  >  1:258434/1‑150 (MQ=255)    ccgccgCAGAACGCGCCTCACTGAAACGTAACCCGTTCCACCCTTTCGCCACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGgcgc                                                                                                                                                >  1:389679/1‑150 (MQ=255)          cAGAACGCGCCTCACTGAAACGTAACCCGTTCCACCCTTTCGCCACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCcgc                                                                                                                                         <  2:540689/151‑1 (MQ=255)           aGAACGCGCCTCACTGAAACGTAACCCGTTCCACCCTTTCGCCACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCcgcg                                                                                                                                        >  2:415238/1‑151 (MQ=255)               cgcgCCTCACTGAAACGTAACCCGTTCCACCCTTTCGCCACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGt                                                                                                                                    <  1:119597/151‑1 (MQ=255)                   ccTCACTGAAACGTAACCCGTTCCACCCTTTCGCCACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACg                                                                                                                                 >  2:136656/1‑150 (MQ=255)                      cACTGAAACGTAACCCGTTCCACCCTTTCGCCACTTTTGACCCTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGGACGacac                                                                                                                             <  1:669256/151‑1 (MQ=255)                         tGAAACGTAACCCGTTCCACCCTTTCGCCACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGCCCTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCg                                                                                                                           >  1:649074/1‑150 (MQ=255)                         tGAAACGTAACCCGTTCCACCCTTTCGCCACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACAcc                                                                                                                            >  1:425401/1‑149 (MQ=255)                          gagtcggagcccGTTCGATCCTGTCGCCACTTTTGACACGGCTGCACTGGCCGGGCGGGCACTCGGACAAACCGTATAGTCAAAGGCTTGCCAGACCGCTGGCGTGGACTTCGACAGCACCTCGGCGCGGTCCGAGCTGTTCGACGGCGaa                                                                                                                         <  1:54921/142‑1 (MQ=255)                             aCGTAACCCGTTCCACCCTTTCGCCACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGc                                                                                                                      <  2:418227/151‑1 (MQ=255)                              cGTAACCCGTTCCACCCTTTCGCCACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGc                                                                                                                      >  1:19999/1‑150 (MQ=255)                              cGTAACCCGTTCCACCCTTTCGCCACTTTTGACACTGCGGCACTGGCCGGGCTGGCACTCTGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATAGAGTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCa                                                                                                                     <  1:644260/151‑1 (MQ=255)                                    ccGTTCCACCCTTTCGCCACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCActgctg                                                                                                               <  1:268801/151‑1 (MQ=255)                                      gTTCCACCCTTTCGCCACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACtgctgtg                                                                                                             <  1:456018/151‑1 (MQ=255)                                        tCCACCCTTTCGCCACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACtgctgtgct                                                                                                           >  1:261017/1‑151 (MQ=255)                                         ccACCCTTTCGCCACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACtgctgtgctg                                                                                                          >  2:379244/1‑151 (MQ=255)                                            cccTTTCGCCACTATTGACACTGCTGCACTGGCCGGGCTGGCGCTCGGACAAACCGAATTGTCAAAGGCTTGCCAGACCGCTGGCATGGGCTTCGACAGCACCCCGGCGCACTCCGCGCTGTTCGACACCGAACGCACTGCTATGCTGtt                                                                                                        <  1:458206/150‑1 (MQ=255)                                                      aCTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCg                                                                                             >  2:644786/1‑151 (MQ=255)                                                      aCTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCg                                                                                             >  2:365838/1‑151 (MQ=255)                                                             acacTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCg                                                                                      >  2:203507/1‑151 (MQ=255)                                                                  gctgcACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTgg                                                                                  >  1:144950/1‑150 (MQ=255)                                                                   ctgcACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGa                                                                                 >  2:45875/1‑150 (MQ=255)                                                                    tgcACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGa                                                                                 >  1:625819/1‑149 (MQ=255)                                                                     gcACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGAAAc                                                                              <  2:320271/151‑1 (MQ=255)                                                                        cTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGAAACGTc                                                                           >  1:27372/1‑151 (MQ=255)                                                                          ggCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACGCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGAAACGTCTg                                                                         <  1:627388/151‑1 (MQ=255)                                                                                  tGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTg                                                                 >  2:443505/1‑151 (MQ=255)                                                                                  tGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTg                                                                 >  2:199470/1‑151 (MQ=255)                                                                                  tGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGGGCTGTTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTg                                                                 >  1:149097/1‑151 (MQ=255)                                                                                     cACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGcc                                                              <  2:357325/151‑1 (MQ=255)                                                                                        tCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCt                                                           >  2:175843/1‑151 (MQ=255)                                                                                                       tGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAag                                             <  2:180991/150‑1 (MQ=255)                                                                                                             aGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGgtgt                                       >  1:333907/1‑150 (MQ=255)                                                                                                             aGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGTTGTa                                      <  1:59163/151‑1 (MQ=255)                                                                                                               gCTTGCCAGACCGCTGGCATGGACTTCGACAGCGCCCCGGCGCACGCCGCGCTGTACGACGCCGAACGCACTGCTGTGCTGTTTTGTGAAAGCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAAt                                    <  1:665225/151‑1 (MQ=255)                                                                                                                    ccAGACCGCTGGCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACATCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAgg                               <  1:152655/151‑2 (MQ=255)                                                                                                                              ggCATGGACTTCGACAGCACCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCtg                      <  2:168346/150‑1 (MQ=255)                                                                                                                                                aCCCCGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAg   <  1:467253/151‑1 (MQ=255)                                                                                                                                                 ccccGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAgg  <  1:48879/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TGGCCGCCGCAGAACGCGCCTCACTGAAACGTAACCCGTTCCACCCTTTCGCCACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGG  >  NC_000913/1728735‑1729029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑