Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913925,9000AG42.6% 184.6 / 33.1 115noncoding (72/88 nt)serWtRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (9/57);  new base G (49/0);  total (58/57)
Fisher's exact test for biased strand distribution p-value = 1.39e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

GAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAA  >  NC_000913/925751‑926049
                                                                                                                                                      |                                                                                                                                                       
   ttCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCg                                                                                                                                                     >  2:77696/1‑151 (MQ=255)
   ttCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAG‑AGGGGGGGGttgg                                                                                                                                                     >  2:588149/1‑149 (MQ=255)
    tCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCg                                                                                                                                                     <  1:1154067/150‑1 (MQ=255)
    tCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCg                                                                                                                                                     <  1:2089684/150‑1 (MQ=255)
     cACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGaa                                                                                                                                                   <  1:25139/151‑1 (MQ=255)
       cTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGG‑GGGGGGGGGGTTGAAcc                                                                                                                                                 >  2:17260/1‑151 (MQ=255)
          ccGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGCT‑TGGGGGGGGTTCGAAacccc                                                                                                                                              >  2:2040037/1‑151 (MQ=255)
                     gCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGGACCCCCCATAC‑Gtt                                                                                                                                     >  1:574309/1‑148 (MQ=255)
                       ttAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAAGGGGGGGGTCAGAACCCCCgtactgtgcc                                                                                                                                    >  1:1379003/1‑140 (MQ=37)
                       ttAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑GGGGGGGGGTTTGAACCCCCCCTAC‑GTTgtc                                                                                                                                  >  2:1954089/1‑147 (MQ=37)
                        tAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGt                                                                                                                                <  1:65162/150‑1 (MQ=255)
                         aGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGG‑AGGGGGGGGTTTGAAACCCCCCTAC‑GTTGCCGtat                                                                                                                              >  2:2158982/1‑151 (MQ=37)
                          gAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGtata                                                                                                                             <  2:1017134/151‑1 (MQ=255)
                          gAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGtata                                                                                                                             <  1:1828154/151‑1 (MQ=255)
                           aaaTTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGtata                                                                                                                             <  2:1238217/150‑1 (MQ=255)
                               tCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTCTCAACCCCGCTAC‑CTTGCCGTATtcaca                                                                                                                         >  2:1144248/1‑150 (MQ=25)
                               tCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAG‑AGGGGGGGGTTTTAACCCCCCGTAC‑GTTGGCGTATaca                                                                                                                           >  2:1268539/1‑148 (MQ=25)
                                cacaGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTCGAACCCCCGATAA‑GTTTCCGGTTaca                                                                                                                           >  1:1206030/1‑147 (MQ=255)
                                cacaGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAG‑AGGGGGGGGTTCGAACCCCCGCTAC‑GTTTCCGTATaca                                                                                                                           >  2:1694130/1‑147 (MQ=255)
                                cacaGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCGCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATacacac                                                                                                                        <  2:1715342/150‑1 (MQ=255)
                                    ggTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTcc                                                                                                                   <  2:318943/151‑1 (MQ=255)
                                     gTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCa                                                                                                                  <  2:1322306/151‑1 (MQ=255)
                                       aaCATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAg                                                                                                                 <  2:2117786/150‑1 (MQ=255)
                                          aTACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTCGAACCCCCCATAC‑GTTGCCGTATACACACTTTCCCgg                                                                                                                >  1:381130/1‑148 (MQ=255)
                                           tACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTTGAAACCCCCATAC‑GTTGCCGTATACACACTTTTCaag                                                                                                                >  1:1151156/1‑145 (MQ=21)
                                           tACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGt                                                                                                             <  2:159609/150‑1 (MQ=255)
                                           tACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGCG‑AGGGGGGGGATAGAAACACCCATAC‑GGTGCCATACACAACCCTTCCAgg                                                                                                                >  2:1436136/1‑147 (MQ=21)
                                            aCTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTCGAACCCCCCAAAA‑GTTGCCGTATAAACACTTTTCAgg                                                                                                                >  2:9207/1‑146 (MQ=21)
                                                 aCCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTCGAAACCCCCATAC‑GTTTCCGTATACAAACCTTTCAGGCGGGCTcc                                                                                                        >  1:1058633/1‑149 (MQ=21)
                                                  cccGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTc                                                                                                     <  2:1517719/151‑1 (MQ=255)
                                                       ccaccaTGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AAGGGGGGGTTCGAACCCCCCAAAC‑GGTGCCCGATACACACTTTCCCGGCGGGCCCCCTCCGccc                                                                                                >  1:731192/1‑150 (MQ=18)
                                                           caTGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGGTTGAAACCCCCATAC‑GTTTCCGTCTACACACTTTCCAGGCGTGCTCCTTCCGcccccc                                                                                             >  1:1689692/1‑146 (MQ=18)
                                                              gTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGa                                                                                          <  1:147934/150‑1 (MQ=255)
                                                                tCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGTTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGaca                                                                                        <  2:524337/150‑1 (MQ=255)
                                                                   cTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCt                                                                                     <  2:33877/150‑1 (MQ=255)
                                                                     gCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGGGCTCTTTTTTAAATATGGCGGTT‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTGCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCa                                                                                   <  2:485881/150‑1 (MQ=255)
                                                                       cGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑a                                                                               <  2:735595/151‑1 (MQ=255)
                                                                        gTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTCGAACCCCCCGTAC‑GGTTCCGTCTAAACACCTTCCAGCCGTGCTCCCTCCGCCACCCCGACACCTccc                                                                                  >  1:266283/1‑146 (MQ=17)
                                                                          acagacagATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTACTCCTTCAGCCACTCGGACACCTCACC‑aaa                                                                             <  1:949526/150‑1 (MQ=255)
                                                                             gacagaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTCGAACCCCCCATAC‑GTTTCCGGATACACACTTTCCCGGCGTGCTCCTTCATCCACCCGGACACCTCACC‑CAAATGt                                                                         >  1:487020/1‑151 (MQ=14)
                                                                             gacagaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGt                                                                         <  1:1779844/151‑1 (MQ=255)
                                                                              acagaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑GGGGGGGGATTCGAACCCCCCATAC‑GTTGCCGTATACACACCTTTCAGGCGGGCTCCTTCCGCCACCCGGGCACCCCACC‑CAAATGtt                                                                        >  1:93617/1‑151 (MQ=14)
                                                                               cagaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCTAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACCCCTCACC‑AAAtt                                                                           <  2:1873683/147‑1 (MQ=255)
                                                                               cagaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGttt                                                                       <  2:260590/151‑1 (MQ=255)
                                                                                  aTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTTGAACCCCCCCTAC‑GTTGCCGGATACACACTTTCCAGGCGTGCTCCCTCCGCCACCCCGACACCCCCCC‑CAAAtttttt                                                                      >  1:920916/1‑149 (MQ=14)
                                                                                     aaaTGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGGTCTAAACCCCCATAA‑GGTGCCGTATAAACACTTTTCAGGCGTGCTCCTTTCGCCACTCGGAAACCCCCCC‑ACAATGtttttgtac                                                                 >  1:1994088/1‑146 (MQ=14)
                                                                                         gCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGGTCGAACCCCCGATAC‑GGTGCCGGATACACACTTTCCCGGCGGGCTCCTTCAGCCACTCGGACACCCCACC‑AAATTGTTTTTCTACCa                                                               >  1:1781176/1‑149 (MQ=14)
                                                                                              aaaaaaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCa                                                        <  2:2002483/151‑1 (MQ=255)
                                                                                              aaaaaaGCTCCCACTTTCCTACCAGCTCTTCTTTAAATATGACCGTA‑AGCCCGCAATTCCAACCCCCCATAC‑GTTGCCTTATACACACTTTCCAGTCGTACTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCa                                                        <  2:2189133/151‑1 (MQ=14)
                                                                                                aaaaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTCGAACCCCCGATAC‑GTTTCCGTATATACACATTCCAGGGGTGCTCCTTCAGCCCCCCGGGCACCTCACC‑AAATTTTTTTGGTAACAAACCTCa                                                        >  1:673841/1‑149 (MQ=14)
                                                                                                aaaaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATg                                                      <  2:329927/151‑1 (MQ=255)
                                                                                                 aaaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCTAACCCCCGCTACGGTTGCC‑TCTAAACACCTTTCCGGCGGGCTCCTTCCGCCACTCGGGCAACTCCCC‑CAAATGTTTTGTTACCCACCCTc                                                         >  2:780481/1‑147 (MQ=9)
                                                                                                  aaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATggg                                                    <  2:1844197/151‑1 (MQ=255)
                                                                                                  aaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACAAAACCTCATggg                                                    <  1:966074/151‑1 (MQ=255)
                                                                                                   aGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGt                                                   <  1:1005727/151‑1 (MQ=255)
                                                                                                    gCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTTGAAACCCCCCAAA‑GTTTCCGTATACACACTTTTCCGGGGGGCTCCCTCAGCCCCTCCGGCACCCCACC‑CAAATTTTTTTCTTCCAAACCCCCTggggg                                                  >  1:1616752/1‑149 (MQ=9)
                                                                                                      tCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑GGGGGGGGGTTTGAAACCCCCATAC‑GTTGCTGTATAAACCCTTTCCAGGCGTGCTCCCTCAGCCACTCGGACACCTCACC‑AAATTGTTTTTCTACCCAAACTcctcggggg                                                 >  2:670373/1‑142 (MQ=11)
                                                                                                      tCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AAGGGGGGGTTCGCCACCCCCCCAC‑GCTGCCGTATACGCACGTTTCCGGGGGGCTCCTTTCCCCCCCCACGCCCCCCACC‑AAAATGTTTTTTTATCAAAccccccggggggg                                                >  2:172790/1‑140 (MQ=2)
                                                                                                       cGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCa                                               <  2:1723656/151‑1 (MQ=255)
                                                                                                        gCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTTGAAACCCCGATAT‑GTTTATGTATAAAAAATTTTCAGGGGGGGTCCTTTAAACACTCGGGCACCTCACC‑AAAATTTTTTTTTAACAAAACTCATggg                                                    >  2:469668/1‑145 (MQ=2)
                                                                                                         cACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAAc                                             <  2:304362/151‑1 (MQ=255)
                                                                                                         cACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAAc                                             <  2:1027497/151‑1 (MQ=255)
                                                                                                           cTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTCGAACCCCCGCTAA‑GTTTGCTCATAAACACTTTCCAGGCGGGCTCCTTCCGCCCCCCCGGCACCCCCCC‑CAATTGTTTTGTTACCAAACCTCCAGGGGGGGAAc                                             >  2:1474141/1‑149 (MQ=9)
                                                                                                           cTTTCGTACGAGCTCTTCTTTAAATATGGCGGAG‑AGGGGGGGGTTTGAAACCCCCATAC‑GTTTGCTTTTAGACACTTTCCCTGCGTGCGCCTTCAGCCACTCCGACACCCCACCAAAATTTTTTTTTAACCAAACC‑CATGGGGGGCAACgg                                           >  1:1519750/1‑151 (MQ=2)
                                                                                                            tttCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTTGAAACCCCCATAC‑GTTTCCGTATACACCCTTTCCAGGCGGGGCCCTTCAGCCACCCGGGCACCCCCCC‑AAAATGTTTTGCTTCCCAACCTCCTGGGGGGCAACggg                                          >  2:210886/1‑151 (MQ=14)
                                                                                                             ttCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg                                          <  1:166717/150‑1 (MQ=255)
                                                                                                             ttCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg                                          <  2:513778/150‑1 (MQ=255)
                                                                                                             ttCGTACGAGCTCTTCTTTAAATATGGCGGGG‑AGGGGGGGGTTCGAAACCCCGGAAC‑GTTGTCGGATACAAACCTTTCAGGGGTGCACCTTCAACCCCTCGGGCACCCCCCC‑CAATTGTTTTTTTACCAAACCCCCTTGGGGGCAAAgggg                                         >  1:2119170/1‑151 (MQ=2)
                                                                                                              tCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg                                          <  1:363513/149‑1 (MQ=255)
                                                                                                               cGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGgc                                        <  1:943851/150‑1 (MQ=255)
                                                                                                                gTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTGGAAACCCCCATAC‑GTTGTCGTATACACACCTTCCCGGGGGGGTCCTTCCACCCCCCCGGCACCACACC‑ACATTGTTTTTCTACCAAACCCCCTGGGTGGCAACGGGgcg                                       >  2:35706/1‑150 (MQ=12)
                                                                                                                gTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGGTTGAACCCCCCATAC‑GTTGCCGTATACACACTTTCCAGGCGGGCTCCCTCAGCCACCCGGACACCCCCCC‑CAATTGTTTTGCTACCCAACCTCCTGGGGGGCAACgggggg                                       >  1:1101541/1‑148 (MQ=17)
                                                                                                                gTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCCATAC‑GTTTCCGTATACACACTTTCCAGGCGGGCTCCTTCAGCCCCTCGGACACCCCACC‑AAAATGTTTTTCTACCAAACCCCCTGGGGGGCAAAgggggg                                       >  1:1661038/1‑148 (MQ=14)
                                                                                                                 tACGAGCTCTTCTTTAAATATGGCGGTG‑GGGGGGGGGTTTGAACCCCCCATAA‑GTTGCCGTGTACACACCTTTCAGGCGTGCTCCCTTCGCCACTCCGACACCTCACC‑CAAATGTTTTTTTACCAAACCCCATGGGGGGCAACgggggg                                       >  1:2153292/1‑147 (MQ=14)
                                                                                                                 tACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTCGAACCCCCGACAC‑GATGCCGTATACAAAATTTCCAGGCGGGCTCCTTCAGCCACTCCGCCACCCCACC‑AAATTTGTTTTCTTCCAAACCCCCTGGGGGGCAACGGGgggc                                      >  1:686818/1‑150 (MQ=14)
                                                                                                                    gAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTTGAACCCCCCATAC‑GTTGTCGGATACACACTTTCCCGGCGGGCTCCTTCAGCCACTCGGGCACCCCCCC‑CAAATGTTTTTCTACCAAACCTCCTTGGGGGCAACCGGGGGccact                                  >  1:833158/1‑151 (MQ=14)
                                                                                                                    gAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTCGAACCCCCCCTAA‑GTTGCCGTATACACACTTTTCAGGCGTGGTCCCTCCGCCACTCGGGCACCCCACC‑CAATTGTTTTGTTACCAAAACTCATGGGGGGGAACGGGGGGcta                                    >  2:640276/1‑149 (MQ=14)
                                                                                                                     aGCTCTTCTTTAAATATGGCGGTG‑GGGGGGGGGGTGGAACCCCCGATAC‑GTTGTCGTATAAACACTTTTCAGGGGGGGTCCTTTCGCCCCTCCGACCACCCACC‑AAATTTTTTTTCTACCAAAACCCATGGGTGGGAAAGGGgggcca                                    >  2:921583/1‑146 (MQ=9)
                                                                                                                     aGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGctact                                  <  2:1378476/150‑1 (MQ=255)
                                                                                                                        tcttctTTAAATATGGCGGTG‑AGGGGGGGGGTTGGACCCCCCCTAC‑GTTTCCGTATTAACAATTTCTAGGCGTGCTCCTTCAGCCCCTCGGGCACCTCCCC‑CAATTGTTTTTCTACCAAACCTCATTGGGGGCAACGGGGGGctacttt                                >  2:1996415/1‑147 (MQ=12)
                                                                                                                         cttcttTAAATATGGCGGTG‑AGGGGGGGGTTTGAAACCCCCCTAC‑TTTTTCGTATACACACTTTTCCGGGGGGCTCCCTCCGCCCCCCGGACACCTCACC‑CAAATTTTTTTTTACCAAAACTCCTGGGGGGCAACGGGGGGctac                                   >  2:2080865/1‑145 (MQ=9)
                                                                                                                              ttAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGa                           <  1:138552/148‑1 (MQ=255)
                                                                                                                              ttAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGt                         <  2:1619813/150‑1 (MQ=255)
                                                                                                                              ttAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGCATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGt                         <  2:1317392/150‑1 (MQ=255)
                                                                                                                               tAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGtt                        <  2:1021105/150‑1 (MQ=255)
                                                                                                                                    atGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAg                    >  1:200257/1‑149 (MQ=255)
                                                                                                                                     tGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGTGCGCTACTATAGGGAGTTGGAGTa                  <  2:1232822/150‑1 (MQ=255)
                                                                                                                                       gCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTaaaa               <  1:370745/151‑1 (MQ=255)
                                                                                                                                         ggTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACg             <  2:1599724/151‑1 (MQ=255)
                                                                                                                                           tG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGt           >  1:627664/1‑151 (MQ=255)
                                                                                                                                            g‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTc          >  2:1334708/1‑151 (MQ=255)
                                                                                                                                               gggggggATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCaa        <  2:1235536/151‑1 (MQ=255)
                                                                                                                                               gggggggATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCaa        <  1:1015808/151‑1 (MQ=255)
                                                                                                                                                ggggggATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCaag       <  2:1082077/151‑1 (MQ=255)
                                                                                                                                                  ggggATTCGACCCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTGCTATAGGGAGTTGGAGTAAAACGGTCaagaa     <  2:539757/151‑1 (MQ=255)
                                                                                                                                                  ggggATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCaagaa     <  1:2078415/151‑1 (MQ=255)
                                                                                                                                                   gggATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCaagaag    <  1:17263/151‑1 (MQ=255)
                                                                                                                                                    ggATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAagaaga   <  1:255149/151‑1 (MQ=255)
                                                                                                                                                     gATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAgaagaa  <  2:731087/151‑1 (MQ=255)
                                                                                                                                                     gATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAgaagaa  <  1:2168651/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
GAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAA  >  NC_000913/925751‑926049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑