Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,356,2010TG41.1% 184.4 / 33.3 108T304P (ACC→CCC) sapAantimicrobial peptide transport ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (9/54);  new base G (44/0);  total (54/54)
Fisher's exact test for biased strand distribution p-value = 1.90e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

GTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCC  >  NC_000913/1356055‑1356351
                                                                                                                                                    |                                                                                                                                                        
gTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTa                                                                                                                                                      <  2:1538611/151‑1 (MQ=255)
   gTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTaaa                                                                                                                                                    <  2:1930682/150‑1 (MQ=255)
   gTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAAt                                                                                                                                                   <  1:539680/151‑1 (MQ=255)
     cATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGc                                                                                                                                                 <  2:843824/151‑1 (MQ=255)
        aGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCa                                                                                                                                               <  1:1366795/150‑1 (MQ=255)
           cccACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATa                                                                                                                                           <  1:172278/151‑1 (MQ=255)
            ccACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTTGGGGGGGTTAAAT‑CCAGATa                                                                                                                                           >  1:1137629/1‑150 (MQ=255)
            ccACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGTTTAATGCCAGa                                                                                                                                             >  2:846473/1‑148 (MQ=255)
              aCGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGc                                                                                                                                        <  1:1475355/151‑1 (MQ=255)
                gAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGa                                                                                                                                      <  1:2104893/151‑1 (MQ=255)
                         ggTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGTTAAATGCCAGAAAAGGGACG‑TTCATcc                                                                                                                            >  1:846949/1‑151 (MQ=255)
                            aaaaTAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCa                                                                                                                          <  1:2091747/150‑1 (MQ=255)
                                  gagGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCa                                                                                                                   <  2:1309395/151‑1 (MQ=255)
                                   agGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTTGGGGTGTTTAATGACAGAGAgg                                                                                                                                         >  2:1911903/1‑129 (MQ=255)
                                      ccgccgTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGTTAAAAGCCCAATAGGCGACG‑GTCATTCCCGGACGCAGCg                                                                                                                >  2:1550706/1‑150 (MQ=255)
                                       cgccgTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTTGCGGGGGTTAATGGCAGAAAAGCGGAG‑TTTCTCCCAgggagccgcggt                                                                                                              >  2:2064886/1‑141 (MQ=255)
                                       cgccgTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGGGGGGTTAAATGCCAGATAAGGGACG‑TTCATCCCCGGGCGCAGc                                                                                                                 >  2:1221036/1‑148 (MQ=255)
                                        gccgTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑GTTTGCGGGGGTAAAAGACAGGAAGGCGAAC‑CTCATTCCAAGGCGCAgg                                                                                                                 >  2:189425/1‑146 (MQ=255)
                                              tCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGc                                                                                                        <  1:372389/150‑1 (MQ=255)
                                               cAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTTGCGGGGTTTAAAGCCCGATAAGGGGCG‑TTCAACCCCGGACGCAGCGTTAAAAGc                                                                                                        >  2:1822089/1‑149 (MQ=255)
                                               cAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAGCGCaa                                                                                                      <  1:1169179/151‑1 (MQ=255)
                                               cAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCa                                                                                                       <  1:284135/150‑1 (MQ=255)
                                                 gCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTTGCGGGGGTAAAAGCCAGATAGGCGCCG‑TTTATCCCAGGACGCCGCGTTaaa                                                                                                           >  2:1582976/1‑144 (MQ=255)
                                                 gCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑GTTTGGGGGGGTAAATGCACGAAAGGGGAAG‑TTTCTCCCAGGGCGCAGCGGTaaa                                                                                                           >  2:2095658/1‑144 (MQ=255)
                                                   cGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGTTTAAATCCAGAAAGGCGACG‑TTTATCCCCGGACGCCGCGGTAAAAGc                                                                                                        >  2:1423347/1‑145 (MQ=255)
                                                    gTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGGGGGGGTAAATACCAGATAGGGGGCG‑GTCATACCCGGAGGCAGCGGTTAAAGCAAg‑cg                                                                                                  >  1:1916710/1‑149 (MQ=255)
                                                      aCCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCggg                                                                                              <  1:1887407/151‑1 (MQ=255)
                                                      aCCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCgg                                                                                               <  1:739134/150‑1 (MQ=255)
                                                       ccATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGgt                                                                                             <  2:1464679/151‑1 (MQ=255)
                                                        cATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGGTAAATGCCAGATAGGGGACG‑TTCATCCCAGGACGCAGCGTTAAAAGCAAG‑CGCggg                                                                                              >  1:2042391/1‑149 (MQ=255)
                                                         ataataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGTTTAATGCCAGAAAAGCGACG‑TTCATCCCCAGACGCAGCGTTAAAAGCAAG‑CGCGGggcg                                                                                           >  1:1584832/1‑151 (MQ=255)
                                                         ataataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGTTAAAAGCCAGATAGGCGACG‑GTCCTCCCCAGACGCAGCGTTAAACGCCAA‑CGCgggg                                                                                             >  2:1062608/1‑148 (MQ=255)
                                                         ataataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGGTTAAAGCCAGAAAGGCGGCG‑GTTATCCCCAGACGCCGGCGTAAAAGCCAA‑CGCggg                                                                                              >  2:1007859/1‑148 (MQ=255)
                                                         ataataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑GTTTGGGGGGTTAAAATCCCGATAAGGGACC‑TTCCTCCCCCGACACCGCGGTAAAAACAAG‑GGCggg                                                                                              >  2:1339220/1‑148 (MQ=255)
                                                           aataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGgtcgtc                                                                                         <  2:743661/151‑1 (MQ=255)
                                                            ataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCa                                                                                        <  2:2023107/151‑1 (MQ=255)
                                                            ataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGGTAAATGCCAGAAAGGGGGCG‑TTCATCCCCGGGCGCCGCGGTAAAACCAAC‑CGCggg                                                                                              >  2:812944/1‑145 (MQ=255)
                                                             taGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTTGCGGGGGTAAATGCCAGATAGGCGGCG‑GTCATCCCCGGACGCAGCGGTAAAAGCAAA‑CGCggg                                                                                              >  1:1794531/1‑144 (MQ=255)
                                                             taGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGGTAAAAGCCCGATAAGGGGCG‑GTCATCCCCGGACGCCGCGGTAAAAGCAAA‑GGCGGGGCGGCAc                                                                                       >  1:1748276/1‑151 (MQ=255)
                                                              aGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACg                                                                                      <  2:1218250/151‑1 (MQ=255)
                                                                aTGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCa                                                                                    <  2:1545458/151‑1 (MQ=255)
                                                                  ggATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCaaa                                                                                  <  1:1982025/151‑1 (MQ=255)
                                                                  ggATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGGTAAATACCAGATAGGCGACG‑GTCATCCCCGGGCGCAGCGTTAAAAGCAAA‑CGCGGGGCGGCACGCaa                                                                                   >  2:435316/1‑150 (MQ=255)
                                                                   gATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCaaaa                                                                                 <  1:610089/151‑1 (MQ=255)
                                                                   gATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCaaaa                                                                                 <  2:732078/151‑1 (MQ=255)
                                                                     ttGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑GTTTGGGGGGTTAAAAGGCAGATAGGGGACG‑TTTATTCCCAGACGCAGCGTTAAAAGCAAG‑CGCGGGGGGTCACGCAAAATg                                                                               >  2:1049864/1‑151 (MQ=255)
                                                                      tGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTTGCGGGGTTAAATGCCAGATAGGGGACG‑GTCATCCCAGGAAGCAGCGGTGAAAGCAAA‑CGCGGGGCGGCACGCaaaaa                                                                                >  2:111537/1‑148 (MQ=255)
                                                                       gCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGa                                                                             <  2:1513474/151‑1 (MQ=255)
                                                                         aTCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATa                                                                           <  2:889186/151‑1 (MQ=255)
                                                                            aGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGTTAAATGCCAGATAGGCGACG‑GTCCTCCCAGGACGCCGCGGTAAAAGCAAA‑CGCGGGGCGTTACGCAAAAAgg                                                                              >  2:1246426/1‑145 (MQ=255)
                                                                             ggCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAgctg                                                                       <  2:458210/151‑1 (MQ=255)
                                                                              gcgcTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAgctgg                                                                      <  2:1725057/151‑1 (MQ=255)
                                                                              gcgcTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGTTAAATGCCAGACAGGCGACC‑TTCTTCCCAGGACGCAGCGGTTAACGCAAA‑CGCGGGGCGGCACGCAAAAAgggaag                                                                          >  2:643825/1‑143 (MQ=255)
                                                                               cgcTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAgctggc                                                                     <  1:1052793/151‑1 (MQ=255)
                                                                                gcTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTTGCGGGGTTAAATGCCAGATAGGGGACG‑GTCATCCCAGGACGCAGCGGTAAACGCAAA‑CGCGGGGCGTCACGCAAAATGGATAactgg                                                                      >  1:1784960/1‑149 (MQ=255)
                                                                                  tGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGTTTAAAGCCAGAAAGGCGGCG‑TTCATCCCAGGACGCAGCGGTAAACGCAAG‑CGCGGGGCGGCACGCCAAATGGATAGctgggtgg                                                                  >  1:375798/1‑151 (MQ=255)
                                                                                  tGGCTATTAACCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑GTTGGCCGTGTTTAATGCCCGATAGGCGACG‑GTCATCCCAAGACACAGCGTTAAAACCAAA‑CGGGGGGCGGCACGCaaaaaaggaaagtggg                                                                     >  2:2142503/1‑136 (MQ=255)
                                                                                     ttattaATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTTGCGGGGTTAAATGCCAGATAGGCGACG‑TTCATCCCCGAGCGCCGCGGTAAACGCCAC‑CGCGGGGCGGCACGCCAAAAGGGAAActggctgg                                                                  >  2:485974/1‑148 (MQ=255)
                                                                                       attaATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGgcagca                                                             <  2:2092375/151‑1 (MQ=255)
                                                                                       attaATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGgcagca                                                             <  1:1620598/151‑1 (MQ=255)
                                                                                         taATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGcagcag                                                            <  2:2079185/150‑1 (MQ=255)
                                                                                          aaTCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTTGCGGGGTTTAAAGCCAGATAAGCGGCG‑TTCATCCCCGGACGCAGCGTTAAACGCAAG‑CGCGGGGCGTCCCGCAAAAAGGGTAGctggctgg                                                                  >  1:25041/1‑143 (MQ=255)
                                                                                           aTCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGG‑TTGGGGGGGGTTAAAGGCC‑GATAGGCGGGG‑GTCATCCCCGGACGCAGCGTTAAAAGCAAG‑CGCGGGGCGGCACGCCAAATGGGTAGCGGGCTGGCAGcaggcc                                                         >  1:544898/1‑151 (MQ=255)
                                                                                            tCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑CTTGGCGGCGGTAAATGCCCCAAATGCGACG‑CTTATTCCCCGGCCCCGCGTTAAACACACG‑AGCGGGGCGGCACGCAAAAAAGATAactggggtcgggcccgcc                                                         >  2:1754751/1‑137 (MQ=255)
                                                                                             cGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGcaggcca                                                        <  1:1972011/150‑1 (MQ=255)
                                                                                                cAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTTGCGGGGTTAAATGCCAGATAGGGGACG‑ATTATCCCCGGACGCAGCGCTTAAAGCCAG‑CGCGGGGCGGCACGCAAAAAAGAGAGCTGGCTGGCAGccgggcagg                                                      >  2:2112288/1‑149 (MQ=255)
                                                                                                cAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCaggccaggcc                                                    <  1:1963353/151‑1 (MQ=255)
                                                                                                 aGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGTTTAATGCCCGATAGGCGGCG‑GTCATCCCCGGACGCAGCGTTAAAAGCAAG‑CGCGGGTCGGCACGCAAAAAGGATAGCTGGCTGGCAGCagggcaggcc                                                    >  1:2144213/1‑150 (MQ=255)
                                                                                                 aGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGGTAAAAGCCAGATAAGCGGCG‑GTCATCCCAGGACGCCGCGTTAAAAGCAAA‑CGCGGGGCGGCACGCAAAAAGGATAGCTGGCTGGCAGCaggccaggcc                                                    >  1:579819/1‑150 (MQ=255)
                                                                                                    gCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGa                                                 <  2:457600/150‑1 (MQ=255)
                                                                                                     ccAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTTGCGGGGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGAAGCAGCGGTAAAAGCAAG‑CGCGGGTCGTCACGCAAAATAGATAGCTGGCTGGCGACCGGGCAGGCCCGaa                                                >  1:386099/1‑150 (MQ=255)
                                                                                                     ccAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGTTAAAAGCCAGATAGGGGACG‑GTCATCCCCCGACGCAACGGTTAACGCAAG‑CGCGGGGTGTCACGCAAAAAAGATAGCTGGCTGGGGGGAAGGCAGGCCAGaa                                                >  1:699726/1‑150 (MQ=255)
                                                                                                      cAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGTTTAATGCCAGATAGGCGACG‑GTCATCCCCGGACGCAGCGTTAAAAGCAAG‑CGCGGGGCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCCGGCCAGaa                                                >  1:1701127/1‑149 (MQ=255)
                                                                                                       aGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTTGCGGTGGTAAAAGCCAGATAGGGGACG‑TTCCTCCCCGGACGCAGCGTTAAAAGCAAA‑GGCGGGTCGCCACGCAAAAAGGAAAGctggctgg                                                                  >  2:931815/1‑130 (MQ=255)
                                                                                                        gcgcATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGGTAAATGGCAGGAAGGGGACGTTTCTCCCCAGGCCG‑AGCGGTAAAAGCAAA‑CGGGGGGCGGCCCGCAAAAAGGGAAGGTGGCTGGGAGCcgggccggcccgga                                                >  2:1415531/1‑143 (MQ=255)
                                                                                                          gcATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGc                                          <  1:1421448/151‑1 (MQ=255)
                                                                                                           cATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCa                                         <  1:1454148/151‑1 (MQ=255)
                                                                                                             tGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTTGTGGGGTTTAAAGCCCGGGAGGGGACG‑TTGATCCCCGGGCCCAGCGTTCAACGCAAA‑CGCGGGGCGGTACGCAAAAAGGATAACTCGCCGGGCGCAGGGCCGGCCaaaa                                                >  2:185218/1‑142 (MQ=255)
                                                                                                              ggCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGGGGGGTTTAATGCCAGAGAGGGGGCG‑GTCATCCCAGGACGCCGGGTTAAAAGCAAG‑AGCGGGGCGGCCCGCAAAAAGGGTAGACGGGTGGGAGGAGGCCCGGCCCCAACGgcgc                                          >  2:1743785/1‑147 (MQ=255)
                                                                                                              ggCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGTTTAAAGCCAGATAGGCGGCG‑GTCATCCCAGGACGCAGCGGTAAAAGCAAG‑CGCGGGGCGTCACGCAAAAAGGATAGCTGGCTGGGAGCAGGCCAGGCCCGAACGTCGCAtt                                       >  1:1695945/1‑150 (MQ=255)
                                                                                                               gCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTTGAGGTGGTAAAAATCAGATAGGAGACG‑CTCAACCCAGGGGGCCGCGTTAAAAGCAAGACCGGGGCCGCCACCAAAAAGGGAAAG‑GCGCTGGCAGCCGGCCCGGCCACAACgaccccttc                                      >  1:763681/1‑142 (MQ=255)
                                                                                                               gCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTcc                                     <  2:749994/151‑1 (MQ=255)
                                                                                                                     aGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTc                               <  2:201096/151‑1 (MQ=255)
                                                                                                                     aGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGGTAAAAACCAGATAGGCGACG‑GTCATCCCCGGACGCCGCGTTAAAAGCAAG‑GGCGGGGGGGCACGCAAAAATGATAGCTGGGTGGCAGCAGGCCCGGCCCGAAAGTCGCATTcccccg                                 >  1:1834711/1‑147 (MQ=255)
                                                                                                                      gCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCa                              <  2:1474781/151‑1 (MQ=255)
                                                                                                                       cGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGTTAAATGCCCGATAAGCGACG‑TTCATCCCCAGACGCAGCGTTAAAAGCAAG‑CGCGGGGCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGGCGCATTcccccg                                 >  1:99831/1‑145 (MQ=255)
                                                                                                                                  aGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACa                  <  1:890831/151‑1 (MQ=255)
                                                                                                                                    cggc‑gg‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGa                <  2:959547/151‑1 (MQ=255)
                                                                                                                                    cggc‑gg‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGa                <  2:1137165/151‑1 (MQ=255)
                                                                                                                                     ggc‑gg‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGAc               <  2:347917/151‑1 (MQ=255)
                                                                                                                                      gc‑gg‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACg              <  2:1345615/151‑1 (MQ=255)
                                                                                                                                      gc‑gg‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACg              <  1:787068/151‑1 (MQ=255)
                                                                                                                                         gg‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTc            >  2:678395/1‑151 (MQ=255)
                                                                                                                                              tGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCgg         <  1:1603765/150‑1 (MQ=255)
                                                                                                                                              tGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGt        >  1:103922/1‑151 (MQ=255)
                                                                                                                                               ggCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTg       <  1:1051756/151‑1 (MQ=255)
                                                                                                                                                 cGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTgcc     <  1:157299/151‑1 (MQ=255)
                                                                                                                                                  ggTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTgccg    >  1:883781/1‑151 (MQ=255)
                                                                                                                                                  ggTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTgcc     <  2:2198065/150‑1 (MQ=255)
                                                                                                                                                    tgtTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTgccgcc  >  2:1001112/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                        
GTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAG‑CGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCC  >  NC_000913/1356055‑1356351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑