Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,376,5050AC43.4% 175.8 / 34.6 106T158P (ACC→CCC) ycjRputative TIM alpha/beta barrel enzyme
Reads supporting (aligned to +/- strand):  ref base A (54/6);  new base C (0/46);  total (54/52)
Fisher's exact test for biased strand distribution p-value = 8.13e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATT  >  NC_000913/1376357‑1376651
                                                                                                                                                         |                                                                                                                                                  
cTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CAcc                                                                                                                                                  >  2:2020206/1‑151 (MQ=255)
 ttACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CAccc                                                                                                                                                 >  1:630687/1‑151 (MQ=255)
 ttACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CAccc                                                                                                                                                 >  1:1011954/1‑151 (MQ=255)
    ccgccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTc                                                                                                                                               <  2:2151621/150‑1 (MQ=255)
    ccgccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCg                                                                                                                                              <  2:624657/151‑1 (MQ=255)
     cgccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGc                                                                                                                                             <  1:1846168/151‑1 (MQ=255)
         gATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCgct                                                                                                                                         >  1:1538969/1‑149 (MQ=255)
            gACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGcc                                                                                                                                      >  1:1822770/1‑151 (MQ=255)
            gACCTCGCCGCGTAGCCGGGACGGCGACCGCAAAAGGGTGAGTGATTCCCTGCGCGTACGGGACCAGGTCGCCGCGCGAACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCGGG‑ATCATATGATCAA‑CACCCTCGCCGAt                                                                                                                                         <  2:1194302/148‑1 (MQ=255)
                 cgccgcGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCccgccg                                                                                                                                 >  1:440441/1‑151 (MQ=255)
                 cgccgcGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCccgccg                                                                                                                                 >  1:1451146/1‑151 (MQ=255)
                   ccgcGTGGCCGGGGCGGCGCCCAAAAAATGGTGAGTGATTCCCTGCGCTTACGGGAACGGGTCGCCGGGCGTCCCGGACCCG‑TGGTGTAT‑CTCAACCCGTAAAC‑CCGCTATCAGG‑ATCATATGATCAC‑CCCCCCCGCCGATGCCCGCCGtt                                                                                                                               <  1:1929115/151‑1 (MQ=255)
                     gcgTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTAc                                                                                                                             >  1:1846322/1‑151 (MQ=255)
                     gcgTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTAc                                                                                                                             >  2:1108631/1‑151 (MQ=255)
                        tAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACAtc                                                                                                                          >  1:2006826/1‑151 (MQ=255)
                          gCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACAtcgt                                                                                                                        >  2:2005505/1‑151 (MQ=255)
                                       ccccAAAGTGGGGAGTGATCCCCCGCGCGTCCGGGAACAGCTCGCCGCGCGTCCCGAACCCG‑TGGTTTAT‑CTCGACCCGTTAAC‑CCGCTATCGTG‑ATCAAATAACCAC‑CCCCCCCGCCGATGCCCGCCGTTACATCGTCGaaaa                                                                                                                  <  2:1607723/141‑1 (MQ=255)
                                       ccGAAAAGTGGTAAGTGACTCCCTGCGCTTACTGGAACAGCGCCCGCCGCGCCCCGGAACGG‑TTGTGTAT‑CTCAACCCGTTAAC‑CCGCTATAAGG‑ATCATAGAACCAA‑CCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAAc                                                                                                                 <  2:470879/145‑1 (MQ=255)
                                           aaaaTGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAAGCGTTAAA‑CCGCTATCAGG‑CTCATATGATCAA‑CACCATCGCCGATGCCCGCCCTTACATCGCCGCCAACAATATTTAAc                                                                                                       >  2:1719915/1‑151 (MQ=255)
                                             aaTGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACAt                                                                                                     >  1:636413/1‑151 (MQ=255)
                                                ggTGAGTGTTCCCCGGCGCTTACTGGAACAGGCCGCCGCGCGTACCGGACCCG‑GGTTGTAT‑CTCGAACCTTAAAC‑CCGCTATCAGG‑ATCATATGATAAA‑CCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATg                                                                                                    <  1:596562/149‑1 (MQ=255)
                                                ggTGAGTGATTCCTGGGGCGTACGGAAACTGGTCGCCGCGCGTGCCGCACCCG‑TGGTGTAT‑CGCCACCCGTAAAC‑CCCCTAT‑AGG‑TTTCTTTTATCAACCACCTTCGCAGGTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGt                                                                                                   <  1:1726734/150‑1 (MQ=255)
                                                  tgagtgaTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTAc                                                                                                 >  1:726925/1‑150 (MQ=255)
                                                  tgagtgaTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTAc                                                                                                 >  2:433406/1‑150 (MQ=255)
                                                  tgagtgaTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTAc                                                                                                 >  2:1661078/1‑150 (MQ=255)
                                                    agtgaTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTAc                                                                                                 >  2:597993/1‑148 (MQ=255)
                                                      tgaTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGAtt                                                                                            >  2:1831341/1‑151 (MQ=255)
                                                       gaTTCCCTGCGCGTACGGGAACAGGTCGCCGCGCGTCCCGGACCCG‑GGGTGTAT‑CTCGACCCTTTAAC‑CCGCTATCAGG‑ATCATATGATCAC‑CCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTa                                                                                           <  1:425188/151‑1 (MQ=255)
                                                        atccccTGCGCGTACTGAAACAGGTCGCCGCGCGTCCCGGAACGG‑TGGTGTAT‑CTCAAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGAtt                                                                                            <  1:647727/146‑1 (MQ=255)
                                                         ttCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATc                                                                                         >  1:273331/1‑151 (MQ=255)
                                                         tccccTGCGCGTCCGGAAACAGGTCCCCGCGCGTCCCGAACCCG‑TGGTGTAT‑CTCGACCCTTAAAC‑CCGCTATAAGG‑ATCATATAATAAA‑CCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTAt                                                                                          <  1:1508121/148‑1 (MQ=255)
                                                           cccTGCGGTTACTGAACCGGGCCCCCGCGCGTACCGGACCCG‑TGTTTTAT‑CTCGACCCGTAAAC‑CCGCTATAAGG‑ATCAAATGAACAA‑CCCCCCCGCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTAt                                                                                          <  2:1789480/148‑1 (MQ=255)
                                                           cccTGCGCTTCTTGGAAGGGGTCCCCGCGCGT‑CCGGGACCG‑GGGTTTTTCCGCGACCTGTAAAC‑CCGCTATCAGA‑ATCAAATAATCAC‑CCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTAt                                                                                          <  2:1368148/148‑1 (MQ=255)
                                                           cccTGCGCGTACGGAAACAGGTCGCCGCGCGTACCGGAACGG‑TGGTTTAT‑CTCAACCCGTTAAC‑CCGCTATCAGG‑ATCATATGATCAA‑CCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGa                                                                                              <  1:649160/144‑1 (MQ=255)
                                                            ccggcgcGTACGGGAACGGGCCGCCGCGCGTCCCGGACCCG‑TGGTTTTC‑CCCGACCCTTTAAC‑CCGCTATAGGA‑ACCATATAATCAC‑CCCCCTCCCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGc                                                                                      <  2:1783994/148‑1 (MQ=255)
                                                            ccTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGc                                                                                      >  2:761526/1‑151 (MQ=255)
                                                              tGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCg                                                                                     >  2:405046/1‑150 (MQ=255)
                                                                     aCTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCTGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTa                                                                              >  1:1848668/1‑150 (MQ=255)
                                                                     aCTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTAt                                                                             >  2:1855678/1‑151 (MQ=255)
                                                                        ggAACAGGCCGCGGCGCGTCCGGAAACCG‑GGGTTTAT‑C‑CCAACCCTTAAACCCTCTATAAGA‑ATAATATAATAAC‑CCCCCCCCCCGAGGGCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATcac                                                                          <  2:1864185/151‑1 (MQ=255)
                                                                          aaCAGGCCGCCGCGCGTCCCGGAACCG‑TGGTTTAT‑CTCAACCCTTTAAC‑CCGCTATCGGT‑ATAATATTATCAA‑CCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACAt                                                                        <  2:1841175/151‑1 (MQ=255)
                                                                           acgggTCGCCGCGCGTCCCGAACCCG‑TGTTGTAT‑CTCAAACCGTTAAC‑CCGCTATCAGG‑ATCATATAATCAA‑CCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATg                                                                       <  1:142002/148‑1 (MQ=255)
                                                                              ggccgccgcGCGTACCGAACCCG‑GGTGGTCT‑CCCGACCCGTAAAC‑CCTCTATCAGG‑ATAATATGACCAC‑CCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGa                                                                      <  2:1659567/146‑1 (MQ=255)
                                                                              ggTCGCCGCGCGTACCGGACCCG‑TGGTGTAT‑CTCGAACCGTTAAC‑CCGCTATCAGG‑ATCATATGATCAA‑CCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATg                                                                       <  1:124521/148‑1 (MQ=255)
                                                                               gccgccgcGCGTCCCGGACCCG‑GGGGGTAT‑CTCAACCCTTTAAC‑CCTCTATAAGG‑ATCATATGATCAC‑CCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAat                                                                    <  1:1874922/148‑1 (MQ=255)
                                                                                  gccgcGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCg                                                                >  2:1883357/1‑151 (MQ=255)
                                                                                    cgcgcgTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCga                                                               >  1:1543926/1‑150 (MQ=255)
                                                                                     gcgcgTCCCGGACCCG‑TGGTTTAT‑CTCGAACCTTTAAC‑CCGCTATCAGG‑ATCATATGATCAA‑CCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCga                                                               <  1:777186/149‑1 (MQ=255)
                                                                                      gggcgtcccgAACCGTTGGTTTTT‑CTCGACCCTTTAAC‑CCGCTATCAGG‑ATCATATAACCAC‑CCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaa                                                              <  2:1796807/141‑1 (MQ=255)
                                                                                       gcgTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATTTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga                                                            >  1:2012013/1‑150 (MQ=255)
                                                                                       gcgTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga                                                            >  1:464521/1‑150 (MQ=255)
                                                                                       gcgTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga                                                            >  2:1864109/1‑150 (MQ=255)
                                                                                        cgTACCGGAACCG‑GGGTGTAT‑CTCGACCCGTTAAC‑CCTCTATCAGG‑ATCATATGATCAC‑CCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga                                                            <  2:640091/149‑1 (MQ=255)
                                                                                          tACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga                                                            >  1:1348288/1‑147 (MQ=255)
                                                                                            ccGGAACCG‑TGGTGTAT‑CTCGAACCGTAAAC‑CCGCTATCAGG‑ATCATATGATCAA‑CCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATaa                                                      <  1:288121/151‑1 (MQ=255)
                                                                                             cGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAAc                                                     >  2:529443/1‑151 (MQ=255)
                                                                                                aaCCG‑TGGTTTAT‑CCCGACCCGTAAAC‑CCGCTATCGGG‑ATCATATGACCAC‑CCCCCTCCCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCt                                                   <  1:744690/150‑1 (MQ=255)
                                                                                                aaCCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTg                                                  >  2:181201/1‑151 (MQ=255)
                                                                                                aaCCG‑GGGTGTAG‑CTCAACCCGTTAAC‑CCGCTAGCAGG‑ATCATAGGACCAC‑CCCCCCCGCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATaa                                                      <  2:1447657/147‑1 (MQ=255)
                                                                                                  ccG‑TGTTGTAT‑CGCGACCCTTTAAC‑CCGCTATCAGG‑TTCATATGATAAA‑CCCCCGCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAAc                                                     <  1:1756212/146‑1 (MQ=255)
                                                                                                    g‑tggtgTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGgcgc                                              >  1:1072404/1‑151 (MQ=255)
                                                                                                    g‑tggtgTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGgcgc                                              >  1:2160411/1‑151 (MQ=255)
                                                                                                    g‑tggtgTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGgcgc                                              >  1:782881/1‑151 (MQ=255)
                                                                                                    g‑tggtgTAT‑CTCGAACCGTAAAC‑CCGCTATCAGG‑ATCATATGATCAA‑CCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTgg                                                 <  1:818536/148‑1 (MQ=255)
                                                                                                     ggggtgTAT‑CGCAAACCGTAAAC‑CCCCTACGAGG‑ATAATATGACCAC‑CCCCCGCGCCGACGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGgc                                                <  2:432360/147‑1 (MQ=255)
                                                                                                       ggttaaT‑CCCGACCCTTTAAC‑CCGCTATCAGG‑ATAATA‑GAACAACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCa                                             <  2:1783346/145‑1 (MQ=255)
                                                                                                       ggtgTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGgcgc                                              >  1:1941605/1‑149 (MQ=255)
                                                                                                         tgtAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgc                                          >  1:489780/1‑151 (MQ=255)
                                                                                                          gtAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgcg                                         >  1:1154199/1‑151 (MQ=255)
                                                                                                           tAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCACAGgcgc                                        >  2:1815486/1‑151 (MQ=255)
                                                                                                                 cGACCCTTTAAC‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTg                                      <  1:448027/148‑1 (MQ=255)
                                                                                                                   atccGTTAAA‑CCGCTATAAGA‑TTCATATGATAAA‑CCCCCCCCAGGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCAt                                   <  1:400657/147‑1 (MQ=255)
                                                                                                                   aaCCGTTAAC‑CCGCTATAAGG‑ATAATAGGACAAA‑CCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGa                                 <  1:1168688/151‑1 (MQ=255)
                                                                                                                   aaCCGTTAAA‑CCGCTATCAGG‑ATCATATGATAAC‑CCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCt                                       <  1:368592/145‑1 (MQ=255)
                                                                                                                    aCCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGAc                                >  2:1362001/1‑151 (MQ=255)
                                                                                                                     ccGTAAAC‑CCGCTA‑CAGGAATCAAATAACCAC‑CCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGAc                                <  2:558945/150‑1 (MQ=255)
                                                                                                                     ccGTAAAC‑CCCCTATCAGA‑AACATCAGACAAA‑CCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCAt                                   <  1:52529/147‑1 (MQ=255)
                                                                                                                        ttAAC‑CCGCTATCAGG‑ATCATATGATCAA‑CCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACaa                              <  1:1248502/149‑1 (MQ=255)
                                                                                                                         tAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcg                           >  2:286301/1‑151 (MQ=255)
                                                                                                                          aaa‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcgc                          >  2:1822805/1‑151 (MQ=255)
                                                                                                                           aacccGCTATCAGG‑ATCATATGATCAA‑CCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcgc                          <  1:575586/148‑1 (MQ=255)
                                                                                                                           aacccGCTACCGGG‑ATCATATGACCAA‑CCCCCCCGCCGGGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAAc                             <  1:407065/145‑1 (MQ=255)
                                                                                                                              ccGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGAc                       >  2:1295285/1‑151 (MQ=255)
                                                                                                                              ccGCTATAAGA‑ATTATATAATCAC‑CCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcgc                          <  2:1148913/148‑1 (MQ=255)
                                                                                                                               cGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGAcc                      >  2:51984/1‑151 (MQ=255)
                                                                                                                                  tATCAGG‑ATCATATAACCAA‑CCCCCCCCCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCt                     <  1:715355/149‑1 (MQ=255)
                                                                                                                                       gg‑ATCATATGATCAA‑CCCCCTCGCCGTTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCg                <  1:554733/149‑1 (MQ=255)
                                                                                                                                       gg‑ATCATATGACCAC‑CCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCt                     <  1:1535420/144‑1 (MQ=255)
                                                                                                                                       agtataatatGATCAA‑CCCCCTCGCCTATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTc                 <  2:1085857/143‑1 (MQ=255)
                                                                                                                                        g‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCCCTACCTGCTCGGTc             >  1:46568/1‑151 (MQ=255)
                                                                                                                                          aTCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTc             >  1:1806686/1‑150 (MQ=255)
                                                                                                                                           tCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAt           >  2:1744117/1‑151 (MQ=255)
                                                                                                                                            cATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtg          >  1:945439/1‑151 (MQ=255)
                                                                                                                                              tatGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtgtg        >  1:1081125/1‑151 (MQ=255)
                                                                                                                                               atGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGc       >  1:184780/1‑151 (MQ=255)
                                                                                                                                                tgctCAA‑CCCCCCCGCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTc             <  1:1364535/141‑1 (MQ=255)
                                                                                                                                                  aTCAA‑CCCCCCCCCCGTGCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtgt         <  2:1259281/146‑1 (MQ=255)
                                                                                                                                                  aTCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCat     >  2:416145/1‑150 (MQ=255)
                                                                                                                                                    cAA‑CCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCa      <  2:102237/147‑1 (MQ=255)
                                                                                                                                                    cAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATAtt  >  2:328994/1‑151 (MQ=255)
                                                                                                                                                         |                                                                                                                                                  
CTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCG‑TGGTGTAT‑CTCGAACCGTTAAA‑CCGCTATCAGG‑ATCATATGATCAA‑CACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATT  >  NC_000913/1376357‑1376651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑