Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,578,1430AC45.7% 160.9 / 39.7 105L79V (TTA→GTA) maeBmalic enzyme: putative oxidoreductase/phosphotransacetylase
Reads supporting (aligned to +/- strand):  ref base A (53/4);  new base C (0/48);  total (53/52)
Fisher's exact test for biased strand distribution p-value = 1.26e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

GGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAA‑CTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCC  >  NC_000913/2577995‑2578291
                                                                                                                                                           |                                                                                                                                                      
 gttCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAA‑CTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑ccc                                                                                                                                                    >  1:996823/1‑151 (MQ=255)
    cGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAA‑CTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCa                                                                                                                                                  <  1:386092/150‑1 (MQ=255)
      aGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAA‑CTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑c                                                                                                                                               >  1:315117/1‑150 (MQ=255)
         gcggcgACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAA‑CTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CAccg                                                                                                                                           >  1:1840728/1‑151 (MQ=255)
              gACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAA‑CTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGt                                                                                                                                       >  1:1691117/1‑150 (MQ=255)
               aCAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAA‑CTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTAc                                                                                                                                     >  2:2004261/1‑151 (MQ=255)
               aCAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAA‑CTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTAc                                                                                                                                     >  1:827911/1‑151 (MQ=255)
                  aCTTCAATAAATTTGTCCGGGTCGAGTTCGTCAA‑CTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGt                                                                                                                                  >  1:1753136/1‑151 (MQ=255)
                     tCAATAAATTTGTCCGGGTCAAGTTCGTCAA‑CTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGtt                                                                                                                                 >  1:338892/1‑149 (MQ=255)
                       aaTAAATTTGTCCGGGTCGAGTTCGTCAA‑CTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTag                                                                                                                               >  1:1271772/1‑149 (MQ=255)
                        aTAAATTTGCCCGGTTCGA‑TTCCTCAACCTTCAATGACAAATACATCAACCCCGGCAAATTTCTAAAACAGACCCCCCTTC‑CCTCCCA‑TCCCCGG‑TTT‑G‑CCGGCCA‑GCGC‑CCAATTTTTCCCAACCCCCAG‑CACCGCCGTACCGTTAgaga                                                                                                                            <  2:2097016/151‑1 (MQ=255)
                              ttGTCCGGGTCGAGTTCGTCAA‑CTTAAATGTCAAATACATCAACCCCGGCAAATTTCTAAAACAGACCGCCCTTG‑CCTCCCA‑CCCCGGG‑TTT‑G‑CCTGCCA‑GCGCGCCATTGTTGCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCAcc                                                                                                                       <  1:1747140/150‑1 (MQ=255)
                               tGTCGGGGTCGAGTTCGTCAA‑CTTCAATGTAAAATACATCAACCCCGGCGATTTTCTAAAACAGACCGCCCTTC‑CCTTCCA‑CCCCCGT‑TTT‑G‑CCTGCCA‑GCGCGCAAATGTTGCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCg                                                                                                                      <  1:2081829/150‑1 (MQ=255)
                               tGTCCGGGTCGAGTTCGTCAA‑CTTCAATGTCAAATACATCAACCCCGGCGAATTTCTTAAACAGACCGCCCTTG‑CCTTCCA‑CCCCCGG‑TTT‑G‑CCGGCCA‑GCGCGCCATTGTTGCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCg                                                                                                                      <  1:166908/150‑1 (MQ=255)
                                gcccGGGTCGAGTTCGCCAT‑CTTCAATGTAAAATACATCAACCCCGGCGAATTTCTTAAGCAAACCCCCCTTC‑CCTCCCA‑CCCCCGT‑TTT‑G‑CCGGCCA‑GCGCGCCATTGTTGCCAAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGc                                                                                                                     <  2:1357366/148‑1 (MQ=255)
                                    gggTCGAGTTCGTCAA‑CTAAAATGTAAAATACATCAACCCCGGCGAATTTCTAAAACAGACCCCCCTTC‑CCTCCCA‑CCCCCGG‑TTT‑G‑CCTGCCG‑GCGCGCAATTGTTCCCAAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGccacca                                                                                                                <  1:1606976/151‑1 (MQ=255)
                                      gTCGAGTTCGTCAA‑CTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAgg                                                                                                              >  1:1927015/1‑151 (MQ=255)
                                       tcgattccttCAA‑CTTCAATAAAAAATCCACAAACCCCGGCGATTTTTTTAAACAGACCGCCCTCG‑CCTCCCA‑TCCCCGT‑TTT‑C‑CCCGCCG‑GCGCGCAAATTTTCCTTAC‑CCCAAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAgg                                                                                                              <  1:22414/141‑1 (MQ=255)
                                        cGAGTTCGTCAA‑CTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGtt                                                                                                            >  1:1195407/1‑151 (MQ=255)
                                         gatttCGTCAA‑CTTAAATGTAAAATACATCAACCCCGGCGATTTTCTTAAACAGAACGCCCTTG‑CCTCCCA‑CCCCCGT‑TTT‑G‑CCTGCCA‑GCGCCCCAATGTTGCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGtt                                                                                                            <  2:293851/147‑1 (MQ=255)
                                         gAGTTCTTAAA‑CTTCAATGTCAAATACACCAACCCCGGCAATTTTCTAAAACAAACCCCCCTTC‑CTTCCCA‑CCCCCGT‑TTT‑G‑CCGGCCG‑GCGCCCCATTGTTCCCCAC‑CCCCAG‑CCCCGCCGTACCGTTAGAGATCACCGCCACCAgg                                                                                                              <  1:1865581/148‑1 (MQ=255)
                                           gTTCGTCAA‑CTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTAcc                                                                                                         >  1:368303/1‑151 (MQ=255)
                                            ttCGTCAA‑CTTCAATGTAAAATACACCAACCCCGGCGATTTTCTAAAACAGACCGCCCTTG‑CCTTCCA‑TCCCCGT‑TTT‑G‑CCGGCCA‑GCGCGCCATTGTTGCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTAc                                                                                                          <  1:921978/149‑1 (MQ=255)
                                            tccttCAA‑CTTAAATGCAAAATACACCACCCCCGGCAATTTCCTAAAACAAAACCCCCTTC‑CCTTCCA‑‑CCCCCG‑TTT‑G‑CCCGGCAGGCGCCCCAATGTTCCCAAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGtt                                                                                                            <  2:423602/143‑1 (MQ=255)
                                              cGTCAA‑CTTCAATGTAAAATACATCAACCCCGGCAAATTTTTAAAAAAGACCGCCCTTC‑CCTCCCA‑CCCCCGT‑TTT‑G‑CCTGCCA‑GCGCGCAAATTTTGCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCg                                                                                                      <  1:918747/151‑1 (MQ=255)
                                                tCAA‑CTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCggg                                                                                                    >  1:1341152/1‑151 (MQ=255)
                                                  aa‑CTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCg                                                                                                  >  1:908436/1‑151 (MQ=255)
                                                   accTTAAATGTAAAATACATCAACCCCGGCGATTTTCTAAAACAGACCGCCCTTG‑CCTCCCA‑CCCCCGT‑TTT‑G‑CCTGCCA‑GCCCGCCAATTTTGCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCggg                                                                                                    <  1:1401213/147‑1 (MQ=255)
                                                   a‑CTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCgg                                                                                                 >  2:2141072/1‑151 (MQ=255)
                                                   a‑CTTAAATGTAAAATACATCAATCCCGGCAAATTTCTAAAACAGACCCCCCTTG‑CCTTCCA‑TCCCCGT‑TTT‑G‑CCTCCCA‑GCGCCCAAATGTGGCCAAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGc                                                                                                   <  1:693716/149‑1 (MQ=255)
                                                      ttaaatttaaaaTACATAACCCCGGGCAATTTTCTAAAACAGACCCCCTTTC‑CCTCCCA‑CCCCCGT‑TTT‑C‑CCGGCCA‑GCGCGCAAATTTTCCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCg                                                                                                      <  1:627631/135‑1 (MQ=255)
                                                         aaTGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTatat                                                                                            >  2:2078378/1‑151 (MQ=255)
                                                         aaTGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTatat                                                                                            >  1:1001542/1‑151 (MQ=255)
                                                         aaTGTCAAATACACCAACCCCGGCAATTTTCTAAAAAAGACCGCCTTTC‑CCTCCCA‑TCCCCGG‑TTT‑G‑CCTGCCG‑GCGCGCCAATTTTCCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTatat                                                                                            <  1:240533/151‑1 (MQ=255)
                                                          aTGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATAtt                                                                                           >  2:1468056/1‑151 (MQ=255)
                                                          aTGTCAAATACATCAACCCCGGCAATTTTCTAAAACAGACCGCCCTTG‑CCTCCCA‑TCCCCGT‑TTT‑G‑CCTGCCA‑GCGCGCCATTTTTCCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGtat                                                                                              <  1:1590468/148‑1 (MQ=255)
                                                           tGTCAAATACATCAACCCCGGCGATTTTCTAAAACAGACCGCCCTTC‑CCTCCCA‑TCCCCGT‑TTT‑C‑CCTGCCA‑GCGCGCCAATGTTGCCTAC‑CCCCAG‑CCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATAtt                                                                                           <  1:1054843/150‑1 (MQ=255)
                                                               aaaTACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGt                                                                                        >  1:971516/1‑149 (MQ=255)
                                                                 acaCATCAACCCCGGCAAATTTCTAAAACAGACCCCCTTTC‑CCTCCCC‑ACCCCGT‑TTT‑G‑CCGCCCA‑GGCCGCCAATGTTGCCTAC‑CCCCAG‑CGCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAgg                                                                                     <  2:1521093/148‑1 (MQ=255)
                                                                  tACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCt                                                                                   >  1:626244/1‑151 (MQ=255)
                                                                  tACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCt                                                                                   >  2:682924/1‑151 (MQ=255)
                                                                   aCATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCtt                                                                                  >  2:2073245/1‑151 (MQ=255)
                                                                    cATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCttt                                                                                 >  2:252895/1‑151 (MQ=255)
                                                                     atcacccccGGCAATTTTTTAAAACAAACCGCCCTTG‑CCTCCCA‑TCCCCGG‑TTT‑G‑CCTGCCA‑GCGCGCAAATGTTCCCTAC‑CCCCAG‑CCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCt                                                                                   <  1:939594/142‑1 (MQ=255)
                                                                     atcacccccGGCAATTTTCTAAAACAGACCCCCTTTC‑CCTCCCA‑TCCCCGT‑TTG‑C‑CCTGCCG‑CCGCCCCATTGTGCCCTAC‑CCCCAG‑CGCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCttt                                                                                 <  2:1942507/144‑1 (MQ=255)
                                                                     accacccccGGCAAATTTTTAAAACA‑AACCCCCTTG‑CCCTCCC‑ACCCCGGTTTT‑C‑CCCGCCA‑GCCCCCCAATGTTCCCTAC‑CCCCGG‑CCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCttt                                                                                 <  2:698941/144‑1 (MQ=255)
                                                                     aTCAACCCCGGCGATTTTCTAAAACAGACCCCCTTTG‑CCTCCCA‑CCCCCGT‑TTT‑C‑CCTCCCA‑GCGCCCAAATGTTCCCAAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCt                                                                                   <  1:807614/148‑1 (MQ=255)
                                                                        acccccGGCAAATTCCTAAAACAGACCCCCCTCC‑CCTCCCA‑CCCCCGG‑TTG‑G‑CCTGCCA‑GCGCGCCAATTTTGCCAAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCt                                                                                   <  2:797338/142‑1 (MQ=255)
                                                                        aacccgggcaattttCTAAAACAGACCGCCTTTC‑CCTCCCA‑CCCCCGG‑TTT‑G‑CCTGCCG‑GCGCGCAATTTTTCCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTa                                                                               <  1:1217899/137‑1 (MQ=255)
                                                                        aaccccGGCGATTTTTTTAAACAGACCCCCCTTG‑CCTTCCA‑TCCCCGT‑TTT‑G‑CCTGCCG‑GCGCGCAAATGTTCCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTaa                                                                              <  1:1818632/147‑1 (MQ=255)
                                                                         aTCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑C‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACg                                                                            >  1:1152636/1‑151 (MQ=255)
                                                                          ccccGGCAATTTTCTAAAACAGCCCCCCCTGG‑CCTCCCA‑CCCCGGG‑TTT‑C‑CCGGCCC‑GCGCGCCAATTTTGCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTa                                                                               <  1:11994/146‑1 (MQ=255)
                                                                           ccgggCAAATTCCTAAAACAGACCCCCGTTC‑CCTCCCT‑CCCCCGG‑TTT‑C‑CCTGCCA‑GCGCACC‑ATTTTGCCCTA‑CCCCAGCCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCtttt                                                                                <  1:588214/142‑1 (MQ=255)
                                                                           cccgggtatttattaaaaCAGACCGCCTTTG‑CCTTCCA‑ACTCCTG‑TTT‑G‑CCTGCCG‑GGGCACCATGTTTGCCTAC‑CCCCAG‑CGCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACgg                                                                           <  2:1781167/135‑1 (MQ=255)
                                                                           cccGGC‑AATTTTTTAAAACAAACCCCCCTC‑CCCTCCACTCCCGGT‑TTT‑C‑CCTGCCG‑CCGCGCAATTTTTCCCAAC‑CCCCAC‑CCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCtttt                                                                                <  2:2043954/145‑1 (MQ=255)
                                                                               gCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCtt                                                                      >  1:298097/1‑151 (MQ=255)
                                                                                    tttCTTAAAAAG‑ACCCCCTTT‑CTCTCCC‑CCCCCCG‑TTT‑GCCCTGCCG‑CCGCCCAAATTTTCCCTAC‑CCCCGC‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCttt                                                                     <  1:1215883/147‑1 (MQ=255)
                                                                                     ttCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCg                                                                 >  2:1123651/1‑150 (MQ=255)
                                                                                          aaaCAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTc                                                            >  1:634692/1‑150 (MQ=255)
                                                                                           aaacaaaCCCCCTTGCCCTCCCA‑CCCCCGT‑TTT‑G‑CCCCCCG‑GCGCGCCAAGGTCCCCAAC‑CCCCAC‑CGCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGAttt                                                             <  2:1196447/144‑1 (MQ=255)
                                                                                           aaaaaaccccccTTC‑CCTCCCA‑CCCCCGG‑TTT‑C‑CCTGCCA‑GCGCGCAATTGTTCCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCaa                                                          <  1:505837/142‑1 (MQ=255)
                                                                                            aCAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCa                                                           >  2:1831378/1‑149 (MQ=255)
                                                                                             cAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCa                                                           >  1:674630/1‑148 (MQ=255)
                                                                                              aGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGAc                                                       >  2:706220/1‑151 (MQ=255)
                                                                                               gAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGAc                                                       >  2:687924/1‑150 (MQ=255)
                                                                                                aaCGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACa                                                      >  2:59009/1‑150 (MQ=255)
                                                                                                   gCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAgg                                                   >  1:494257/1‑150 (MQ=255)
                                                                                                     ccctgcccTCCCA‑CCCCCGG‑TTT‑G‑CCTCCCA‑GCGCGCAAAGGTTCCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTg                                                 <  2:343961/145‑1 (MQ=255)
                                                                                                         g‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGggcggc                                             >  2:396168/1‑150 (MQ=255)
                                                                                                          cccTCCCA‑CCCCCGT‑TTT‑C‑CCTCCCA‑GCGCGCAATTGTTCCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCa                                            <  2:160895/150‑1 (MQ=255)
                                                                                                           ccTCCCA‑TCCCCGG‑TTT‑G‑CCTCCCG‑GCGCGCCAATGTTCCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCa                                            <  1:334309/150‑1 (MQ=255)
                                                                                                            cttcccacccccGG‑TTT‑G‑CCCCCCA‑GCGCGCCATTGTTGCCTAC‑CCCCAG‑CCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCg                                               <  2:68027/137‑1 (MQ=255)
                                                                                                             ttCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACg                                         >  1:448044/1‑151 (MQ=255)
                                                                                                             ttCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACg                                         >  1:479983/1‑151 (MQ=255)
                                                                                                             ttCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACg                                         >  2:1957272/1‑151 (MQ=255)
                                                                                                             ttCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACg                                         >  1:1364524/1‑151 (MQ=255)
                                                                                                             ttCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACg                                         >  1:642056/1‑151 (MQ=255)
                                                                                                                ccacccccGT‑TTT‑C‑CCTCCCG‑GCGCCCAATTGTTCCCAAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAAc                                          <  1:2000648/142‑1 (MQ=255)
                                                                                                                ccacccccGG‑TTT‑C‑CCGGCCA‑GCGCCCCATTGTTCCCAAC‑CCCCAG‑CGCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCaa                                           <  2:380433/141‑1 (MQ=255)
                                                                                                                     cccggtttt‑C‑CCGGCCG‑GCGCGCAATTTTTGCCAAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCt                                      <  1:1130180/142‑1 (MQ=255)
                                                                                                                      ccGG‑TTT‑G‑CCTGCCG‑GCGCCCAAATGTTGCCTAC‑CCCCAC‑CCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGa                                 <  2:459720/151‑1 (MQ=255)
                                                                                                                       ccg‑TTT‑GCCCTGCGA‑GCGCGCAAATTTTCCCTAC‑CCCCAC‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTg                                  <  1:1773723/148‑1 (MQ=255)
                                                                                                                       cGG‑TTTCG‑CCCGCCG‑GCGCCCCAGTTTTCCCAAC‑CCCCAG‑CCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTg                                     <  2:1099767/147‑1 (MQ=255)
                                                                                                                        gg‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGt                               >  2:1953845/1‑151 (MQ=255)
                                                                                                                                 cctgccA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGcc                          >  1:124039/1‑150 (MQ=255)
                                                                                                                                 cctgccA‑GCGCCCCATTTTGGCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAgg                            <  2:104726/148‑1 (MQ=255)
                                                                                                                                      cA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGcc                    >  1:1616135/1‑151 (MQ=255)
                                                                                                                                      cA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGcc                    >  1:471047/1‑151 (MQ=255)
                                                                                                                                             gccaataccaccTAA‑CCCCGC‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGTAACGCCTGGTGAGTAGGCCAGCGCCAg                  <  2:2194003/137‑1 (MQ=255)
                                                                                                                                              ccAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATcgc             >  1:1165179/1‑151 (MQ=255)
                                                                                                                                              caaaTGTGCCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGa                 <  1:494031/145‑1 (MQ=255)
                                                                                                                                                aaTTTTGCCTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATcgcg            <  1:718500/150‑1 (MQ=255)
                                                                                                                                                 aTGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCt          >  2:1915994/1‑151 (MQ=255)
                                                                                                                                                  ttttGCCAAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATcgcg            <  1:413338/146‑1 (MQ=255)
                                                                                                                                                  tGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCtg         >  2:437331/1‑151 (MQ=255)
                                                                                                                                                     tGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTgtgt      >  1:606868/1‑151 (MQ=255)
                                                                                                                                                       ccTAC‑CCCCAG‑CCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCa                         <  2:1276900/130‑1 (MQ=255)
                                                                                                                                                       ccTAC‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATc               <  2:1352598/140‑1 (MQ=255)
                                                                                                                                                         tAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGcc  >  1:816215/1‑151 (MQ=255)
                                                                                                                                                         tAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGcc  >  1:1395166/1‑151 (MQ=255)
                                                                                                                                                           |                                                                                                                                                      
GGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAA‑CTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTG‑CCTTCCA‑TCACCGG‑TTT‑G‑CCTGCCA‑GCGCGCCAATGTTGCCTAA‑CCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCC  >  NC_000913/2577995‑2578291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑